Restriction map for
pF1KB6274
(Linear) (Six-base) MAPSORT of: KIBB6274.seq Check: 7254 from: 1 to: 2091
KIBB6274.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 712 1035 1462 2091
Size: 613 99 323 427 629
Fragments arranged by size:
629 613 427 323 99
AfeI AGC'GCT
Cuts at: 0 1006 1958 2091
Size: 1006 952 133
Fragments arranged by size:
1006 952 133
AflIII A'CryG_T
Cuts at: 0 1200 2091
Size: 1200 891
AleI CACnn'nnGTG
Cuts at: 0 1189 2023 2091
Size: 1189 834 68
Fragments arranged by size:
1189 834 68
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2066 2091
Size: 2066 25
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1805 1837 2091
Size: 1805 32 254
Fragments arranged by size:
1805 254 32
AlwNI CAG_nnn'CTG
Cuts at: 0 152 189 262 1804 2077 2091
Size: 152 37 73 1542 273 14
Fragments arranged by size:
1542 273 152 73 37 14
ApaI G_GGCC'C
Cuts at: 0 16 274 955 2091
Size: 16 258 681 1136
Fragments arranged by size:
1136 681 258 16
ApaLI G'TGCA_C
Cuts at: 0 373 412 1826 2041 2091
Size: 373 39 1414 215 50
Fragments arranged by size:
1414 373 215 50 39
ApoI r'AATT_y
Cuts at: 0 235 2091
Size: 235 1856
AvaI C'yCGr_G
Cuts at: 0 1522 1736 2091
Size: 1522 214 355
Fragments arranged by size:
1522 355 214
BanI G'GyrC_C
Cuts at: 0 476 804 1950 2091
Size: 476 328 1146 141
Fragments arranged by size:
1146 476 328 141
BanII G_rGCy'C
Cuts at: 0 16 72 274 501 955 1117 1127 1364
Size: 16 56 202 227 454 162 10 237
Cuts at: 1364 1798 2091
Size: 434 293
Fragments arranged by size:
454 434 293 237 227 202 162 56
16 10
BbeI G_GCGC'C
Cuts at: 0 480 808 1954 2091
Size: 480 328 1146 137
Fragments arranged by size:
1146 480 328 137
BbsI GAAGACnn'nnnn_
Cuts at: 0 637 1509 2091
Size: 637 872 582
Fragments arranged by size:
872 637 582
BbvCI CC'TCA_GC
Cuts at: 0 1469 2091
Size: 1469 622
BglI GCCn_nnn'nGGC
Cuts at: 0 1907 1916 2091
Size: 1907 9 175
Fragments arranged by size:
1907 175 9
BglII A'GATC_T
Cuts at: 0 1139 2091
Size: 1139 952
BlpI GC'TnA_GC
Cuts at: 0 718 2091
Size: 718 1373
Bme1580I G_kGCm'C
Cuts at: 0 16 274 377 416 440 955 1830 2045
Size: 16 258 103 39 24 515 875 215
Cuts at: 2045 2084 2091
Size: 39 7
Fragments arranged by size:
875 515 258 215 103 39 39 24
16 7
BmgBI CAC'GTC
Cuts at: 0 1155 2091
Size: 1155 936
BmrI ACTGGGnnnn_n'
Cuts at: 0 747 1863 2065 2091
Size: 747 1116 202 26
Fragments arranged by size:
1116 747 202 26
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 880 1952 2091
Size: 508 372 1072 139
Fragments arranged by size:
1072 508 372 139
Bpu10I CC'TnA_GC
Cuts at: 0 1469 1797 2091
Size: 1469 328 294
Fragments arranged by size:
1469 328 294
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 2091
Size: 784 1307
BsaI GGTCTCn'nnnn_
Cuts at: 0 574 667 2091
Size: 574 93 1424
Fragments arranged by size:
1424 574 93
BsaAI yAC'GTr
Cuts at: 0 522 2025 2091
Size: 522 1503 66
Fragments arranged by size:
1503 522 66
BsaHI Gr'CG_yC
Cuts at: 0 477 785 805 1032 1951 2091
Size: 477 308 20 227 919 140
Fragments arranged by size:
919 477 308 227 140 20
BsaWI w'CCGG_w
Cuts at: 0 743 2091
Size: 743 1348
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 337 367 733 763 835 865 1672 1702
Size: 337 30 366 30 72 30 807 30
Cuts at: 1702 2091
Size: 389
Fragments arranged by size:
807 389 366 337 72 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 349 856 883 1693 1696 2091
Size: 349 507 27 810 3 395
Fragments arranged by size:
810 507 395 349 27 3
BseYI C'CCAG_C
Cuts at: 0 262 1212 1253 1344 2091
Size: 262 950 41 91 747
Fragments arranged by size:
950 747 262 91 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 1021 1167 2091
Size: 138 883 146 924
Fragments arranged by size:
924 883 146 138
BsiEI CG_ry'CG
Cuts at: 0 1236 2091
Size: 1236 855
BsiHKAI G_wGCw'C
Cuts at: 0 377 416 1127 1830 2045 2091
Size: 377 39 711 703 215 46
Fragments arranged by size:
711 703 377 215 46 39
BsmBI CGTCTCn'nnnn_
Cuts at: 0 792 2091
Size: 792 1299
Bsp1286I G_dGCh'C
Cuts at: 0 16 72 274 377 416 440 501 955
Size: 16 56 202 103 39 24 61 454
Cuts at: 955 1117 1127 1364 1798 1830 2045 2084 2091
Size: 162 10 237 434 32 215 39 7
Fragments arranged by size:
454 434 237 215 202 162 103 61
56 39 39 32 24 16 10 7
BsrBI CCG'CTC
Cuts at: 0 118 939 1860 2091
Size: 118 821 921 231
Fragments arranged by size:
921 821 231 118
BsrDI GCAATG_nn'
Cuts at: 0 199 1577 2091
Size: 199 1378 514
Fragments arranged by size:
1378 514 199
BsrFI r'CCGG_y
Cuts at: 0 416 713 864 1235 2091
Size: 416 297 151 371 856
Fragments arranged by size:
856 416 371 297 151
BstAPI GCAn_nnn'nTGC
Cuts at: 0 152 189 389 1544 2091
Size: 152 37 200 1155 547
Fragments arranged by size:
1155 547 200 152 37
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1594 2091
Size: 1594 497
BstYI r'GATC_y
Cuts at: 0 491 515 1139 1877 2091
Size: 491 24 624 738 214
Fragments arranged by size:
738 624 491 214 24
BtgI C'CryG_G
Cuts at: 0 471 809 1745 2091
Size: 471 338 936 346
Fragments arranged by size:
936 471 346 338
BtsI GCAGTG_nn'
Cuts at: 0 150 2091
Size: 150 1941
DrdI GACnn_nn'nnGTC
Cuts at: 0 1159 1978 2091
Size: 1159 819 113
Fragments arranged by size:
1159 819 113
EaeI y'GGCC_r
Cuts at: 0 869 1100 1233 1748 1908 1985 2091
Size: 869 231 133 515 160 77 106
Fragments arranged by size:
869 515 231 160 133 106 77
EagI C'GGCC_G
Cuts at: 0 1233 2091
Size: 1233 858
EarI CTCTTCn'nnn_
Cuts at: 0 963 1408 2091
Size: 963 445 683
Fragments arranged by size:
963 683 445
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 298 2091
Size: 298 1793
EcoICRI GAG'CTC
Cuts at: 0 1125 2091
Size: 1125 966
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 613 712 880 1035 1462 1952 2091
Size: 508 105 99 168 155 427 490 139
Fragments arranged by size:
508 490 427 168 155 139 105 99
EcoO109I rG'GnC_Cy
Cuts at: 0 13 40 186 270 271 952 1225 1437
Size: 13 27 146 84 1 681 273 212
Cuts at: 1437 1550 1801 2091
Size: 113 251 290
Fragments arranged by size:
681 290 273 251 212 146 113 84
27 13 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1026 2091
Size: 1026 1065
FseI GG_CCGG'CC
Cuts at: 0 1239 2091
Size: 1239 852
HaeII r_GCGC'y
Cuts at: 0 263 423 471 480 714 808 1008 1213
Size: 263 160 48 9 234 94 200 205
Cuts at: 1213 1791 1954 1960 2091
Size: 578 163 6 131
Fragments arranged by size:
578 263 234 205 200 163 160 131
94 48 9 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 483 515 733 765 1600 1632 1862 1869
Size: 483 32 218 32 835 32 230 7
Cuts at: 1869 1894 1901 2091
Size: 25 7 190
Fragments arranged by size:
835 483 230 218 190 32 32 32
25 7 7
KasI G'GCGC_C
Cuts at: 0 476 804 1950 2091
Size: 476 328 1146 141
Fragments arranged by size:
1146 476 328 141
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1070 2091
Size: 1070 1021
MscI TGG'CCA
Cuts at: 0 1750 1987 2091
Size: 1750 237 104
Fragments arranged by size:
1750 237 104
MslI CAynn'nnrTG
Cuts at: 0 1189 2023 2048 2091
Size: 1189 834 25 43
Fragments arranged by size:
1189 834 43 25
MspA1I CmG'CkG
Cuts at: 0 473 486 1344 1844 1916 2091
Size: 473 13 858 500 72 175
Fragments arranged by size:
858 500 473 175 72 13
NaeI GCC'GGC
Cuts at: 0 715 1237 2091
Size: 715 522 854
Fragments arranged by size:
854 715 522
NarI GG'CG_CC
Cuts at: 0 477 805 1951 2091
Size: 477 328 1146 140
Fragments arranged by size:
1146 477 328 140
NgoMIV G'CCGG_C
Cuts at: 0 713 1235 2091
Size: 713 522 856
Fragments arranged by size:
856 713 522
NspI r_CATG'y
Cuts at: 0 1503 2091
Size: 1503 588
PasI CC'CwG_GG
Cuts at: 0 1780 2091
Size: 1780 311
PflMI CCAn_nnn'nTGG
Cuts at: 0 262 2091
Size: 262 1829
PfoI T'CCnGG_A
Cuts at: 0 1026 1163 2091
Size: 1026 137 928
Fragments arranged by size:
1026 928 137
PpuMI rG'GwC_Cy
Cuts at: 0 40 1225 1437 1550 2091
Size: 40 1185 212 113 541
Fragments arranged by size:
1185 541 212 113 40
PspOMI G'GGCC_C
Cuts at: 0 12 270 951 2091
Size: 12 258 681 1140
Fragments arranged by size:
1140 681 258 12
PstI C_TGCA'G
Cuts at: 0 148 208 485 889 2091
Size: 148 60 277 404 1202
Fragments arranged by size:
1202 404 277 148 60
PvuII CAG'CTG
Cuts at: 0 486 1344 2091
Size: 486 858 747
Fragments arranged by size:
858 747 486
RsrII CG'GwC_CG
Cuts at: 0 1281 2091
Size: 1281 810
SacI G_AGCT'C
Cuts at: 0 1127 2091
Size: 1127 964
SacII CC_GC'GG
Cuts at: 0 474 2091
Size: 474 1617
SanDI GG'GwC_CC
Cuts at: 0 40 1550 2091
Size: 40 1510 541
Fragments arranged by size:
1510 541 40
SfcI C'TryA_G
Cuts at: 0 144 204 321 481 885 1431 2091
Size: 144 60 117 160 404 546 660
Fragments arranged by size:
660 546 404 160 144 117 60
SfoI GGC'GCC
Cuts at: 0 478 806 1952 2091
Size: 478 328 1146 139
Fragments arranged by size:
1146 478 328 139
SgrAI Cr'CCGG_yG
Cuts at: 0 416 2091
Size: 416 1675
SmaI CCC'GGG
Cuts at: 0 1524 1738 2091
Size: 1524 214 353
Fragments arranged by size:
1524 353 214
SmlI C'TyrA_G
Cuts at: 0 799 2091
Size: 799 1292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1267 1793 2091
Size: 1267 526 298
Fragments arranged by size:
1267 526 298
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 337 369 2091
Size: 337 32 1722
Fragments arranged by size:
1722 337 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 526 2091
Size: 526 1565
XmaI C'CCGG_G
Cuts at: 0 1522 1736 2091
Size: 1522 214 355
Fragments arranged by size:
1522 355 214
XmnI GAAnn'nnTTC
Cuts at: 0 1516 2091
Size: 1516 575
Enzymes that do cut:
AcuI AfeI AflIII AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI
BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BsrFI
BstAPI BstXI BstYI BtgI BtsI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I FalI FseI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PstI
PvuII RsrII SacI SacII SanDI SfcI SfoI SgrAI
SmaI SmlI TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI
BclI BfrBI BmtI BplI BsaBI BsiWI BsmI BspEI
BspHI BspMI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII EcoNI EcoRI
EcoRV FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI SalI SapI SbfI ScaI SexAI SfiI SgfI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TatI Tth111I XbaI XhoI ZraI