Restriction map for
pF1KB6278
(Linear) (Six-base) MAPSORT of: KIBB6278.seq Check: 8356 from: 1 to: 2343
KIBB6278.orf
With 182 enzymes: SgfI *
December 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1495 2343
Size: 1495 848
AccI GT'mk_AC
Cuts at: 0 1599 2343
Size: 1599 744
Acc65I G'GTAC_C
Cuts at: 0 1834 2343
Size: 1834 509
AclI AA'CG_TT
Cuts at: 0 1393 2343
Size: 1393 950
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 557 1079 1087 1154 1300 1691 2343
Size: 412 145 522 8 67 146 391 652
Fragments arranged by size:
652 522 412 391 146 145 67 8
AflII C'TTAA_G
Cuts at: 0 1725 2343
Size: 1725 618
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2158 2343
Size: 2158 185
AlwNI CAG_nnn'CTG
Cuts at: 0 2323 2343
Size: 2323 20
ApaI G_GGCC'C
Cuts at: 0 261 2343
Size: 261 2082
ApoI r'AATT_y
Cuts at: 0 432 608 1425 2038 2343
Size: 432 176 817 613 305
Fragments arranged by size:
817 613 432 305 176
AseI AT'TA_AT
Cuts at: 0 869 959 2343
Size: 869 90 1384
Fragments arranged by size:
1384 869 90
AvaI C'yCGr_G
Cuts at: 0 837 2343
Size: 837 1506
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 782 815 2343
Size: 782 33 1528
Fragments arranged by size:
1528 782 33
BanI G'GyrC_C
Cuts at: 0 1834 2343
Size: 1834 509
BanII G_rGCy'C
Cuts at: 0 261 1920 2343
Size: 261 1659 423
Fragments arranged by size:
1659 423 261
BbsI GAAGACnn'nnnn_
Cuts at: 0 1906 2343
Size: 1906 437
BglII A'GATC_T
Cuts at: 0 1098 2343
Size: 1098 1245
Bme1580I G_kGCm'C
Cuts at: 0 261 2343
Size: 261 2082
BmgBI CAC'GTC
Cuts at: 0 51 2343
Size: 51 2292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1511 1958 2343
Size: 1511 447 385
Fragments arranged by size:
1511 447 385
BsaHI Gr'CG_yC
Cuts at: 0 1468 2343
Size: 1468 875
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 319 349 1509 1539 2343
Size: 319 30 1160 30 804
Fragments arranged by size:
1160 804 319 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 247 250 323 2343
Size: 247 3 73 2020
Fragments arranged by size:
2020 247 73 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 2343
Size: 281 2062
BsiEI CG_ry'CG
Cuts at: 0 245 2343
Size: 245 2098
BsiHKAI G_wGCw'C
Cuts at: 0 315 1920 2343
Size: 315 1605 423
Fragments arranged by size:
1605 423 315
BsmI GAATG_Cn'
Cuts at: 0 1626 1932 2343
Size: 1626 306 411
Fragments arranged by size:
1626 411 306
Bsp1286I G_dGCh'C
Cuts at: 0 261 315 1920 2343
Size: 261 54 1605 423
Fragments arranged by size:
1605 423 261 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 202 537 1074 1495 2343
Size: 202 335 537 421 848
Fragments arranged by size:
848 537 421 335 202
BsrBI CCG'CTC
Cuts at: 0 7 174 942 2343
Size: 7 167 768 1401
Fragments arranged by size:
1401 768 167 7
BsrGI T'GTAC_A
Cuts at: 0 1182 2343
Size: 1182 1161
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1428 2343
Size: 1428 915
BstXI CCAn_nnnn'nTGG
Cuts at: 0 323 2343
Size: 323 2020
BstYI r'GATC_y
Cuts at: 0 524 1098 2343
Size: 524 574 1245
Fragments arranged by size:
1245 574 524
BstZ17I GTA'TAC
Cuts at: 0 1600 2343
Size: 1600 743
BtgI C'CryG_G
Cuts at: 0 87 1263 2343
Size: 87 1176 1080
Fragments arranged by size:
1176 1080 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 200 2343
Size: 200 2143
EaeI y'GGCC_r
Cuts at: 0 84 185 242 2343
Size: 84 101 57 2101
Fragments arranged by size:
2101 101 84 57
EagI C'GGCC_G
Cuts at: 0 242 2343
Size: 242 2101
EarI CTCTTCn'nnn_
Cuts at: 0 576 1008 1130 1667 1824 2343
Size: 576 432 122 537 157 519
Fragments arranged by size:
576 537 519 432 157 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2250 2343
Size: 2250 93
EcoICRI GAG'CTC
Cuts at: 0 1918 2343
Size: 1918 425
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 557 1079 1087 1154 1300 1511 1691
Size: 412 145 522 8 67 146 211 180
Cuts at: 1691 1958 2343
Size: 267 385
Fragments arranged by size:
522 412 385 267 211 180 146 145
67 8
EcoO109I rG'GnC_Cy
Cuts at: 0 257 258 2343
Size: 257 1 2085
Fragments arranged by size:
2085 257 1
EcoRV GAT'ATC
Cuts at: 0 2280 2343
Size: 2280 63
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2299 2343
Size: 2299 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 319 351 1893 1905 1925 1937 2343
Size: 319 32 1542 12 20 12 406
Fragments arranged by size:
1542 406 319 32 20 12 12
KpnI G_GTAC'C
Cuts at: 0 1838 2343
Size: 1838 505
MfeI C'AATT_G
Cuts at: 0 719 2343
Size: 719 1624
MslI CAynn'nnrTG
Cuts at: 0 2027 2343
Size: 2027 316
MspA1I CmG'CkG
Cuts at: 0 145 1326 2343
Size: 145 1181 1017
Fragments arranged by size:
1181 1017 145
NcoI C'CATG_G
Cuts at: 0 1263 2343
Size: 1263 1080
NotI GC'GGCC_GC
Cuts at: 0 242 2343
Size: 242 2101
PflMI CCAn_nnn'nTGG
Cuts at: 0 691 1882 2343
Size: 691 1191 461
Fragments arranged by size:
1191 691 461
PfoI T'CCnGG_A
Cuts at: 0 1332 1611 2343
Size: 1332 279 732
Fragments arranged by size:
1332 732 279
PspOMI G'GGCC_C
Cuts at: 0 257 2343
Size: 257 2086
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2238 2270 2343
Size: 2238 32 73
Fragments arranged by size:
2238 73 32
PstI C_TGCA'G
Cuts at: 0 104 199 864 1638 2190 2343
Size: 104 95 665 774 552 153
Fragments arranged by size:
774 665 552 153 104 95
PvuII CAG'CTG
Cuts at: 0 1326 2343
Size: 1326 1017
SacI G_AGCT'C
Cuts at: 0 1920 2343
Size: 1920 423
SapI GCTCTTCn'nnn_
Cuts at: 0 576 2343
Size: 576 1767
SfcI C'TryA_G
Cuts at: 0 100 195 860 1166 1583 1634 2186 2343
Size: 100 95 665 306 417 51 552 157
Fragments arranged by size:
665 552 417 306 157 100 95 51
SmlI C'TyrA_G
Cuts at: 0 1725 2343
Size: 1725 618
SspI AAT'ATT
Cuts at: 0 452 789 1155 2217 2343
Size: 452 337 366 1062 126
Fragments arranged by size:
1062 452 366 337 126
StyI C'CwwG_G
Cuts at: 0 44 1263 2343
Size: 44 1219 1080
Fragments arranged by size:
1219 1080 44
TatI w'GTAC_w
Cuts at: 0 1033 1182 1401 1731 1967 2137 2343
Size: 1033 149 219 330 236 170 206
Fragments arranged by size:
1033 330 236 219 206 170 149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1244 1276 1509 1541 1956 1988 2343
Size: 1244 32 233 32 415 32 355
Fragments arranged by size:
1244 415 355 233 32 32 32
XbaI T'CTAG_A
Cuts at: 0 819 2343
Size: 819 1524
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 535 2343
Size: 535 1808
XmnI GAAnn'nnTTC
Cuts at: 0 657 2343
Size: 657 1686
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AlfI AlwNI
ApaI ApoI AseI AvaI BaeI BanI BanII BbsI
BglII Bme1580I BmgBI BpmI BsaHI BsaXI BseRI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrGI BstAPI
BstXI BstYI BstZ17I BtgI BtgZI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRV FalI Hin4I KpnI
MfeI MslI MspA1I NcoI NotI PflMI PfoI PspOMI
PsrI PstI PvuII SacI SapI SfcI SmlI SspI
StyI TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII AfeI AflIII AgeI AhdI AleI AloI ApaLI
AscI AsiSI AvrII BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BseYI BsiWI BsmBI
BspEI BspHI BsrDI BsrFI BssHII BssSI BstBI BstEII
Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EcoNI
EcoRI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI MluI MmeI MscI NaeI NarI NdeI NgoMIV
NheI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI TaqII Tth111I XhoI XmaI ZraI