Restriction map for
pF1KB6279
(Linear) (Six-base) MAPSORT of: KIBB6279.seq Check: 8105 from: 1 to: 2382
KIBB6279.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 0 106 413 2382
Size: 106 307 1969
Fragments arranged by size:
1969 307 106
Acc65I G'GTAC_C
Cuts at: 0 1474 2382
Size: 1474 908
AclI AA'CG_TT
Cuts at: 0 444 2382
Size: 444 1938
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 1662 1709 1715 2382
Size: 625 1037 47 6 667
Fragments arranged by size:
1037 667 625 47 6
AflIII A'CryG_T
Cuts at: 0 564 1799 2382
Size: 564 1235 583
Fragments arranged by size:
1235 583 564
AleI CACnn'nnGTG
Cuts at: 0 515 2382
Size: 515 1867
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 545 1967 2382
Size: 545 1422 415
Fragments arranged by size:
1422 545 415
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 227 259 429 461 2180 2212 2382
Size: 227 32 170 32 1719 32 170
Fragments arranged by size:
1719 227 170 170 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1212 1566 1629 1693 2382
Size: 1212 354 63 64 689
Fragments arranged by size:
1212 689 354 64 63
ApaI G_GGCC'C
Cuts at: 0 1361 2382
Size: 1361 1021
ApoI r'AATT_y
Cuts at: 0 1719 1989 2293 2302 2382
Size: 1719 270 304 9 80
Fragments arranged by size:
1719 304 270 80 9
AseI AT'TA_AT
Cuts at: 0 1184 2382
Size: 1184 1198
AvaI C'yCGr_G
Cuts at: 0 1875 2382
Size: 1875 507
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 350 383 616 649 1682 1715 2382
Size: 350 33 233 33 1033 33 667
Fragments arranged by size:
1033 667 350 233 33 33 33
BanI G'GyrC_C
Cuts at: 0 1474 2382
Size: 1474 908
BanII G_rGCy'C
Cuts at: 0 1126 1361 2182 2382
Size: 1126 235 821 200
Fragments arranged by size:
1126 821 235 200
BbsI GAAGACnn'nnnn_
Cuts at: 0 56 561 2382
Size: 56 505 1821
Fragments arranged by size:
1821 505 56
BbvCI CC'TCA_GC
Cuts at: 0 2144 2382
Size: 2144 238
BclI T'GATC_A
Cuts at: 0 1332 2382
Size: 1332 1050
Bme1580I G_kGCm'C
Cuts at: 0 1361 2382
Size: 1361 1021
BmrI ACTGGGnnnn_n'
Cuts at: 0 1355 2382
Size: 1355 1027
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 1234 1994 2382
Size: 137 1097 760 388
Fragments arranged by size:
1097 760 388 137
Bpu10I CC'TnA_GC
Cuts at: 0 2144 2382
Size: 2144 238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 2382
Size: 287 2095
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 2382
Size: 265 2117
BseYI C'CCAG_C
Cuts at: 0 2150 2335 2382
Size: 2150 185 47
Fragments arranged by size:
2150 185 47
BsiHKAI G_wGCw'C
Cuts at: 0 524 2182 2205 2382
Size: 524 1658 23 177
Fragments arranged by size:
1658 524 177 23
BsmI GAATG_Cn'
Cuts at: 0 1924 2382
Size: 1924 458
Bsp1286I G_dGCh'C
Cuts at: 0 524 1126 1361 2182 2205 2382
Size: 524 602 235 821 23 177
Fragments arranged by size:
821 602 524 235 177 23
BspHI T'CATG_A
Cuts at: 0 840 1590 2382
Size: 840 750 792
Fragments arranged by size:
840 792 750
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 644 1935 2382
Size: 644 1291 447
Fragments arranged by size:
1291 644 447
BsrDI GCAATG_nn'
Cuts at: 0 534 1326 2382
Size: 534 792 1056
Fragments arranged by size:
1056 792 534
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1090 2382
Size: 1090 1292
BstEII G'GTnAC_C
Cuts at: 0 1740 2382
Size: 1740 642
BstXI CCAn_nnnn'nTGG
Cuts at: 0 75 2377 2382
Size: 75 2302 5
Fragments arranged by size:
2302 75 5
BstYI r'GATC_y
Cuts at: 0 167 2382
Size: 167 2215
BtgI C'CryG_G
Cuts at: 0 1538 2382
Size: 1538 844
BtsI GCAGTG_nn'
Cuts at: 0 522 931 2246 2382
Size: 522 409 1315 136
Fragments arranged by size:
1315 522 409 136
DrdI GACnn_nn'nnGTC
Cuts at: 0 1945 2382
Size: 1945 437
EaeI y'GGCC_r
Cuts at: 0 99 2367 2382
Size: 99 2268 15
Fragments arranged by size:
2268 99 15
EarI CTCTTCn'nnn_
Cuts at: 0 266 1247 1935 2382
Size: 266 981 688 447
Fragments arranged by size:
981 688 447 266
EcoICRI GAG'CTC
Cuts at: 0 2180 2382
Size: 2180 202
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 625 1234 1662 1709 1715 1994 2382
Size: 137 488 609 428 47 6 279 388
Fragments arranged by size:
609 488 428 388 279 137 47 6
EcoO109I rG'GnC_Cy
Cuts at: 0 1357 2382
Size: 1357 1025
EcoRI G'AATT_C
Cuts at: 0 1989 2382
Size: 1989 393
EcoRV GAT'ATC
Cuts at: 0 224 1673 2382
Size: 224 1449 709
Fragments arranged by size:
1449 709 224
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 895 2040 2382
Size: 895 1145 342
Fragments arranged by size:
1145 895 342
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 208 240 466 498 851 883 952 984
Size: 208 32 226 32 353 32 69 32
Cuts at: 984 2382
Size: 1398
Fragments arranged by size:
1398 353 226 208 69 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 2165 2382
Size: 2165 217
KpnI G_GTAC'C
Cuts at: 0 1478 2382
Size: 1478 904
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1613 1740 2382
Size: 1613 127 642
Fragments arranged by size:
1613 642 127
MscI TGG'CCA
Cuts at: 0 101 2369 2382
Size: 101 2268 13
Fragments arranged by size:
2268 101 13
MslI CAynn'nnrTG
Cuts at: 0 515 527 581 597 665 2382
Size: 515 12 54 16 68 1717
Fragments arranged by size:
1717 515 68 54 16 12
MspA1I CmG'CkG
Cuts at: 0 1510 2382
Size: 1510 872
NdeI CA'TA_TG
Cuts at: 0 722 1248 2382
Size: 722 526 1134
Fragments arranged by size:
1134 722 526
NspI r_CATG'y
Cuts at: 0 568 618 1803 2382
Size: 568 50 1185 579
Fragments arranged by size:
1185 579 568 50
PciI A'CATG_T
Cuts at: 0 564 1799 2382
Size: 564 1235 583
Fragments arranged by size:
1235 583 564
PfoI T'CCnGG_A
Cuts at: 0 67 2382
Size: 67 2315
PspOMI G'GGCC_C
Cuts at: 0 1357 2382
Size: 1357 1025
PstI C_TGCA'G
Cuts at: 0 180 1625 1829 2382
Size: 180 1445 204 553
Fragments arranged by size:
1445 553 204 180
PvuII CAG'CTG
Cuts at: 0 1510 2382
Size: 1510 872
SacI G_AGCT'C
Cuts at: 0 2182 2382
Size: 2182 200
SapI GCTCTTCn'nnn_
Cuts at: 0 266 2382
Size: 266 2116
ScaI AGT'ACT
Cuts at: 0 137 1056 1383 2382
Size: 137 919 327 999
Fragments arranged by size:
999 919 327 137
SfcI C'TryA_G
Cuts at: 0 124 176 310 1621 1825 2382
Size: 124 52 134 1311 204 557
Fragments arranged by size:
1311 557 204 134 124 52
SmlI C'TyrA_G
Cuts at: 0 302 2382
Size: 302 2080
StyI C'CwwG_G
Cuts at: 0 2309 2382
Size: 2309 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 76 1172 2382
Size: 76 1096 1210
Fragments arranged by size:
1210 1096 76
TatI w'GTAC_w
Cuts at: 0 135 1054 1381 2382
Size: 135 919 327 1001
Fragments arranged by size:
1001 919 327 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 311 343 2382
Size: 311 32 2039
Fragments arranged by size:
2039 311 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1654 2382
Size: 1654 728
XmnI GAAnn'nnTTC
Cuts at: 0 296 1989 2382
Size: 296 1693 393
Fragments arranged by size:
1693 393 296
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AloI
AlwNI ApaI ApoI AseI AvaI BaeI BanI BanII
BbsI BbvCI BclI Bme1580I BmrI BpmI Bpu10I BpuEI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BstAPI BstEII BstXI BstYI BtgI BtsI DrdI EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I
HindIII KpnI MmeI MscI MslI MspA1I NdeI NspI
PciI PfoI PspOMI PstI PvuII SacI SapI ScaI
SfcI SmlI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI ApaLI AscI
AsiSI AvrII BamHI BbeI BcgI BciVI BfrBI BglI
BglII BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EagI EciI EcoNI
FseI FspI FspAI HaeII HincII HpaI KasI MfeI
MluI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XhoI XmaI ZraI