Restriction map for
pF1KB6281
(Linear) (Six-base) MAPSORT of: KIBB6281.seq Check: 5236 from: 1 to: 2394
KIBB6281.orf
With 182 enzymes: SgfI *
June 25, 2015 11:41 ..
AatII G_ACGT'C
Cuts at: 0 615 2394
Size: 615 1779
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 967 1954 2394
Size: 967 987 440
Fragments arranged by size:
987 967 440
AflII C'TTAA_G
Cuts at: 0 430 849 2394
Size: 430 419 1545
Fragments arranged by size:
1545 430 419
AflIII A'CryG_T
Cuts at: 0 11 2394
Size: 11 2383
AgeI A'CCGG_T
Cuts at: 0 1441 2394
Size: 1441 953
AhdI GACnn_n'nnGTC
Cuts at: 0 30 2394
Size: 30 2364
ApaLI G'TGCA_C
Cuts at: 0 2115 2394
Size: 2115 279
ApoI r'AATT_y
Cuts at: 0 558 1503 1842 2394
Size: 558 945 339 552
Fragments arranged by size:
945 558 552 339
AseI AT'TA_AT
Cuts at: 0 1169 1193 2394
Size: 1169 24 1201
Fragments arranged by size:
1201 1169 24
BanI G'GyrC_C
Cuts at: 0 391 447 969 2394
Size: 391 56 522 1425
Fragments arranged by size:
1425 522 391 56
BanII G_rGCy'C
Cuts at: 0 1211 2394
Size: 1211 1183
BbsI GAAGACnn'nnnn_
Cuts at: 0 80 314 998 1120 2394
Size: 80 234 684 122 1274
Fragments arranged by size:
1274 684 234 122 80
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 40 74 1045 1079 2394
Size: 40 34 971 34 1315
Fragments arranged by size:
1315 971 40 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 132 2197 2394
Size: 132 2065 197
Fragments arranged by size:
2065 197 132
BglII A'GATC_T
Cuts at: 0 93 2394
Size: 93 2301
Bme1580I G_kGCm'C
Cuts at: 0 2119 2394
Size: 2119 275
BmgBI CAC'GTC
Cuts at: 0 348 2394
Size: 348 2046
BmrI ACTGGGnnnn_n'
Cuts at: 0 1121 1400 2394
Size: 1121 279 994
Fragments arranged by size:
1121 994 279
BmtI G_CTAG'C
Cuts at: 0 268 2394
Size: 268 2126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1754 2394
Size: 1754 640
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 2394
Size: 148 2246
BsaI GGTCTCn'nnnn_
Cuts at: 0 493 1335 1772 2394
Size: 493 842 437 622
Fragments arranged by size:
842 622 493 437
BsaHI Gr'CG_yC
Cuts at: 0 612 2394
Size: 612 1782
BsaWI w'CCGG_w
Cuts at: 0 1441 2394
Size: 1441 953
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 882 912 1193 1223 2394
Size: 882 30 281 30 1171
Fragments arranged by size:
1171 882 281 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 650 887 890 1236 2125 2394
Size: 650 237 3 346 889 269
Fragments arranged by size:
889 650 346 269 237 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 2394
Size: 192 2202
BsiHKAI G_wGCw'C
Cuts at: 0 1211 2119 2394
Size: 1211 908 275
Fragments arranged by size:
1211 908 275
BsmI GAATG_Cn'
Cuts at: 0 484 2394
Size: 484 1910
BsmBI CGTCTCn'nnnn_
Cuts at: 0 338 428 2394
Size: 338 90 1966
Fragments arranged by size:
1966 338 90
Bsp1286I G_dGCh'C
Cuts at: 0 1211 2119 2394
Size: 1211 908 275
Fragments arranged by size:
1211 908 275
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1317 2207 2394
Size: 1317 890 187
Fragments arranged by size:
1317 890 187
BsrFI r'CCGG_y
Cuts at: 0 1441 2394
Size: 1441 953
BssSI C'ACGA_G
Cuts at: 0 1102 1924 2394
Size: 1102 822 470
Fragments arranged by size:
1102 822 470
BstEII G'GTnAC_C
Cuts at: 0 1144 2394
Size: 1144 1250
BstYI r'GATC_y
Cuts at: 0 93 1780 1792 2394
Size: 93 1687 12 602
Fragments arranged by size:
1687 602 93 12
BtgI C'CryG_G
Cuts at: 0 1517 2394
Size: 1517 877
BtsI GCAGTG_nn'
Cuts at: 0 180 489 2394
Size: 180 309 1905
Fragments arranged by size:
1905 309 180
DrdI GACnn_nn'nnGTC
Cuts at: 0 792 2394
Size: 792 1602
EarI CTCTTCn'nnn_
Cuts at: 0 239 410 574 782 1791 1930 2015 2394
Size: 239 171 164 208 1009 139 85 379
Fragments arranged by size:
1009 379 239 208 171 164 139 85
EcoICRI GAG'CTC
Cuts at: 0 1209 2394
Size: 1209 1185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 967 1754 1954 2394
Size: 967 787 200 440
Fragments arranged by size:
967 787 440 200
EcoO109I rG'GnC_Cy
Cuts at: 0 1709 2394
Size: 1709 685
EcoRI G'AATT_C
Cuts at: 0 558 2394
Size: 558 1836
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 456 2394
Size: 456 1938
FspI TGC'GCA
Cuts at: 0 2215 2389 2394
Size: 2215 174 5
Fragments arranged by size:
2215 174 5
HaeII r_GCGC'y
Cuts at: 0 2028 2394
Size: 2028 366
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 573 605 1231 1263 1636 1668
Size: 16 32 525 32 626 32 373 32
Cuts at: 1668 1683 1715 2284 2316 2394
Size: 15 32 569 32 78
Fragments arranged by size:
626 569 525 373 78 32 32 32
32 32 32 16 15
HincII GTy'rAC
Cuts at: 0 592 2394
Size: 592 1802
HindIII A'AGCT_T
Cuts at: 0 2382 2394
Size: 2382 12
MfeI C'AATT_G
Cuts at: 0 542 2015 2394
Size: 542 1473 379
Fragments arranged by size:
1473 542 379
NcoI C'CATG_G
Cuts at: 0 1517 2394
Size: 1517 877
NheI G'CTAG_C
Cuts at: 0 264 2394
Size: 264 2130
NspI r_CATG'y
Cuts at: 0 15 2394
Size: 15 2379
PciI A'CATG_T
Cuts at: 0 11 2394
Size: 11 2383
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1851 1883 2394
Size: 1851 32 511
Fragments arranged by size:
1851 511 32
PpuMI rG'GwC_Cy
Cuts at: 0 1709 2394
Size: 1709 685
PshAI GACnn'nnGTC
Cuts at: 0 1725 2394
Size: 1725 669
PsiI TTA'TAA
Cuts at: 0 473 1501 2394
Size: 473 1028 893
Fragments arranged by size:
1028 893 473
PstI C_TGCA'G
Cuts at: 0 876 2394
Size: 876 1518
SacI G_AGCT'C
Cuts at: 0 1211 2394
Size: 1211 1183
SapI GCTCTTCn'nnn_
Cuts at: 0 410 2015 2394
Size: 410 1605 379
Fragments arranged by size:
1605 410 379
SfcI C'TryA_G
Cuts at: 0 872 1821 1881 2394
Size: 872 949 60 513
Fragments arranged by size:
949 872 513 60
SmlI C'TyrA_G
Cuts at: 0 163 430 849 2394
Size: 163 267 419 1545
Fragments arranged by size:
1545 419 267 163
StuI AGG'CCT
Cuts at: 0 878 2394
Size: 878 1516
StyI C'CwwG_G
Cuts at: 0 809 1517 1570 2394
Size: 809 708 53 824
Fragments arranged by size:
824 809 708 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 145 903 1387 1674 2394
Size: 145 758 484 287 720
Fragments arranged by size:
758 720 484 287 145
TatI w'GTAC_w
Cuts at: 0 203 2243 2394
Size: 203 2040 151
Fragments arranged by size:
2040 203 151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 882 914 1193 1225 2394
Size: 882 32 279 32 1169
Fragments arranged by size:
1169 882 279 32 32
XbaI T'CTAG_A
Cuts at: 0 1219 2394
Size: 1219 1175
ZraI GAC'GTC
Cuts at: 0 613 2394
Size: 613 1781
Enzymes that do cut:
AatII AcuI AflII AflIII AgeI AhdI ApaLI ApoI
AseI BanI BanII BbsI BcgI BciVI BglII Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrFI BssSI BstEII BstYI BtgI BtsI DrdI EarI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII MfeI NcoI NheI NspI PciI PpiI
PpuMI PshAI PsiI PstI SacI SapI SfcI SmlI
StuI StyI TaqII TatI TstI XbaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AleI AlfI AloI
AlwNI ApaI AscI AsiSI AvaI AvrII BaeI BamHI
BbeI BbvCI BclI BfrBI BglI BlpI BplI Bpu10I
BsaAI BsaBI BseYI BsiEI BsiWI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EaeI EagI EciI
EcoNI EcoRV FseI FspAI HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XcmI XhoI XmaI XmnI