Restriction map for
pF1KB6284
(Linear) (Six-base) MAPSORT of: KIBB6284.seq Check: 2044 from: 1 to: 2349
KIBB6284.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
Acc65I G'GTAC_C
Cuts at: 0 740 1367 2349
Size: 740 627 982
Fragments arranged by size:
982 740 627
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 679 2349
Size: 583 96 1670
Fragments arranged by size:
1670 583 96
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 44 1250 1301 2349
Size: 44 1206 51 1048
Fragments arranged by size:
1206 1048 51 44
AlwNI CAG_nnn'CTG
Cuts at: 0 462 808 975 1212 1870 1895 2202 2292
Size: 462 346 167 237 658 25 307 90
Cuts at: 2292 2349
Size: 57
Fragments arranged by size:
658 462 346 307 237 167 90 57
25
ApaI G_GGCC'C
Cuts at: 0 345 1081 1153 1409 1854 2349
Size: 345 736 72 256 445 495
Fragments arranged by size:
736 495 445 345 256 72
ApaLI G'TGCA_C
Cuts at: 0 2020 2349
Size: 2020 329
ApoI r'AATT_y
Cuts at: 0 2160 2349
Size: 2160 189
AseI AT'TA_AT
Cuts at: 0 244 2349
Size: 244 2105
AvaI C'yCGr_G
Cuts at: 0 764 1187 2323 2349
Size: 764 423 1136 26
Fragments arranged by size:
1136 764 423 26
BamHI G'GATC_C
Cuts at: 0 599 687 1545 2349
Size: 599 88 858 804
Fragments arranged by size:
858 804 599 88
BanI G'GyrC_C
Cuts at: 0 619 662 740 1367 1608 1661 2349
Size: 619 43 78 627 241 53 688
Fragments arranged by size:
688 627 619 241 78 53 43
BanII G_rGCy'C
Cuts at: 0 345 572 1081 1153 1194 1224 1409 1854
Size: 345 227 509 72 41 30 185 445
Cuts at: 1854 1988 2349
Size: 134 361
Fragments arranged by size:
509 445 361 345 227 185 134 72
41 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 1017 2349
Size: 1017 1332
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1799 1833 2349
Size: 1799 34 516
Fragments arranged by size:
1799 516 34
BclI T'GATC_A
Cuts at: 0 632 1782 2349
Size: 632 1150 567
Fragments arranged by size:
1150 632 567
BglI GCCn_nnn'nGGC
Cuts at: 0 465 2349
Size: 465 1884
BlpI GC'TnA_GC
Cuts at: 0 443 801 2349
Size: 443 358 1548
Fragments arranged by size:
1548 443 358
Bme1580I G_kGCm'C
Cuts at: 0 345 622 1081 1153 1301 1409 1613 1846
Size: 345 277 459 72 148 108 204 233
Cuts at: 1846 1854 2024 2349
Size: 8 170 325
Fragments arranged by size:
459 345 325 277 233 204 170 148
108 72 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 963 1288 1722 1870 1895 2051 2349
Size: 963 325 434 148 25 156 298
Fragments arranged by size:
963 434 325 298 156 148 25
BmtI G_CTAG'C
Cuts at: 0 371 2349
Size: 371 1978
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 852 2349
Size: 852 1497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 316 847 1178 1358 1380 1872 2093
Size: 212 104 531 331 180 22 492 221
Cuts at: 2093 2349
Size: 256
Fragments arranged by size:
531 492 331 256 221 212 180 104
22
Bpu10I CC'TnA_GC
Cuts at: 0 1401 1501 1859 2349
Size: 1401 100 358 490
Fragments arranged by size:
1401 490 358 100
BsaHI Gr'CG_yC
Cuts at: 0 1883 2349
Size: 1883 466
BsaWI w'CCGG_w
Cuts at: 0 659 1717 2349
Size: 659 1058 632
Fragments arranged by size:
1058 659 632
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 511 541 2349
Size: 511 30 1808
Fragments arranged by size:
1808 511 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 410 844 2180 2329 2341 2349
Size: 410 434 1336 149 12 8
Fragments arranged by size:
1336 434 410 149 12 8
BseYI C'CCAG_C
Cuts at: 0 462 1223 1244 1550 1760 2025 2300 2349
Size: 462 761 21 306 210 265 275 49
Fragments arranged by size:
761 462 306 275 265 210 49 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1112 1163 1428 2349
Size: 1112 51 265 921
Fragments arranged by size:
1112 921 265 51
BsiHKAI G_wGCw'C
Cuts at: 0 2024 2349
Size: 2024 325
Bsp1286I G_dGCh'C
Cuts at: 0 345 572 622 1081 1153 1194 1224 1301
Size: 345 227 50 459 72 41 30 77
Cuts at: 1301 1409 1613 1846 1854 1988 2024 2349
Size: 108 204 233 8 134 36 325
Fragments arranged by size:
459 345 325 233 227 204 134 108
77 72 50 41 36 30 8
BsrDI GCAATG_nn'
Cuts at: 0 279 2349
Size: 279 2070
BsrFI r'CCGG_y
Cuts at: 0 1658 2349
Size: 1658 691
BsrGI T'GTAC_A
Cuts at: 0 527 2349
Size: 527 1822
BstAPI GCAn_nnn'nTGC
Cuts at: 0 662 2267 2349
Size: 662 1605 82
Fragments arranged by size:
1605 662 82
BstXI CCAn_nnnn'nTGG
Cuts at: 0 825 1693 2349
Size: 825 868 656
Fragments arranged by size:
868 825 656
BstYI r'GATC_y
Cuts at: 0 599 687 1333 1545 1798 2349
Size: 599 88 646 212 253 551
Fragments arranged by size:
646 599 551 253 212 88
BtgI C'CryG_G
Cuts at: 0 1470 2349
Size: 1470 879
BtsI GCAGTG_nn'
Cuts at: 0 1100 1335 1368 1512 2280 2349
Size: 1100 235 33 144 768 69
Fragments arranged by size:
1100 768 235 144 69 33
DrdI GACnn_nn'nnGTC
Cuts at: 0 1435 2349
Size: 1435 914
EaeI y'GGCC_r
Cuts at: 0 9 475 580 728 775 1038 2349
Size: 9 466 105 148 47 263 1311
Fragments arranged by size:
1311 466 263 148 105 47 9
EarI CTCTTCn'nnn_
Cuts at: 0 1425 2349
Size: 1425 924
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 316 583 679 847 1178 1358 1380
Size: 212 104 267 96 168 331 180 22
Cuts at: 1380 1872 2093 2349
Size: 492 221 256
Fragments arranged by size:
492 331 267 256 221 212 180 168
104 96 22
EcoO109I rG'GnC_Cy
Cuts at: 0 342 979 1078 1150 1316 1405 1406 1850
Size: 342 637 99 72 166 89 1 444
Cuts at: 1850 1851 1975 2146 2227 2349
Size: 1 124 171 81 122
Fragments arranged by size:
637 444 342 171 166 124 122 99
89 81 72 1 1
FspI TGC'GCA
Cuts at: 0 655 2349
Size: 655 1694
HaeII r_GCGC'y
Cuts at: 0 1212 2349
Size: 1212 1137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1004 1036 1384 1416 2349
Size: 1004 32 348 32 933
Fragments arranged by size:
1004 933 348 32 32
HincII GTy'rAC
Cuts at: 0 1440 1881 2349
Size: 1440 441 468
Fragments arranged by size:
1440 468 441
KpnI G_GTAC'C
Cuts at: 0 744 1371 2349
Size: 744 627 978
Fragments arranged by size:
978 744 627
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 1826 2349
Size: 305 1521 523
Fragments arranged by size:
1521 523 305
MscI TGG'CCA
Cuts at: 0 11 477 582 730 777 2349
Size: 11 466 105 148 47 1572
Fragments arranged by size:
1572 466 148 105 47 11
MslI CAynn'nnrTG
Cuts at: 0 628 1232 2349
Size: 628 604 1117
Fragments arranged by size:
1117 628 604
MspA1I CmG'CkG
Cuts at: 0 18 24 103 261 450 462 726 752
Size: 18 6 79 158 189 12 264 26
Cuts at: 752 794 2349
Size: 42 1555
Fragments arranged by size:
1555 264 189 158 79 42 26 18
12 6
NcoI C'CATG_G
Cuts at: 0 1470 2349
Size: 1470 879
NheI G'CTAG_C
Cuts at: 0 367 2349
Size: 367 1982
NspI r_CATG'y
Cuts at: 0 939 1566 2349
Size: 939 627 783
Fragments arranged by size:
939 783 627
PasI CC'CwG_GG
Cuts at: 0 975 1847 2349
Size: 975 872 502
Fragments arranged by size:
975 872 502
PflMI CCAn_nnn'nTGG
Cuts at: 0 975 1114 1557 1870 2202 2349
Size: 975 139 443 313 332 147
Fragments arranged by size:
975 443 332 313 147 139
PfoI T'CCnGG_A
Cuts at: 0 1084 1194 2349
Size: 1084 110 1155
Fragments arranged by size:
1155 1084 110
PpuMI rG'GwC_Cy
Cuts at: 0 979 1975 2349
Size: 979 996 374
Fragments arranged by size:
996 979 374
PspOMI G'GGCC_C
Cuts at: 0 341 1077 1149 1405 1850 2349
Size: 341 736 72 256 445 499
Fragments arranged by size:
736 499 445 341 256 72
PspXI vC'TCGA_Gb
Cuts at: 0 2323 2349
Size: 2323 26
PstI C_TGCA'G
Cuts at: 0 23 65 455 757 799 1495 1534 2349
Size: 23 42 390 302 42 696 39 815
Fragments arranged by size:
815 696 390 302 42 42 39 23
PvuII CAG'CTG
Cuts at: 0 18 24 450 726 752 794 2349
Size: 18 6 426 276 26 42 1555
Fragments arranged by size:
1555 426 276 42 26 18 6
SanDI GG'GwC_CC
Cuts at: 0 979 1975 2349
Size: 979 996 374
Fragments arranged by size:
996 979 374
SapI GCTCTTCn'nnn_
Cuts at: 0 1425 2349
Size: 1425 924
SbfI CC_TGCA'GG
Cuts at: 0 65 2349
Size: 65 2284
SexAI A'CCwGG_T
Cuts at: 0 1002 1113 2349
Size: 1002 111 1236
Fragments arranged by size:
1236 1002 111
SfcI C'TryA_G
Cuts at: 0 19 61 451 753 795 1459 1491 1530
Size: 19 42 390 302 42 664 32 39
Cuts at: 1530 2349
Size: 819
Fragments arranged by size:
819 664 390 302 42 42 39 32
19
SmaI CCC'GGG
Cuts at: 0 766 1189 2349
Size: 766 423 1160
Fragments arranged by size:
1160 766 423
SmlI C'TyrA_G
Cuts at: 0 2323 2349
Size: 2323 26
StuI AGG'CCT
Cuts at: 0 1737 2114 2349
Size: 1737 377 235
Fragments arranged by size:
1737 377 235
StyI C'CwwG_G
Cuts at: 0 1470 1595 2071 2349
Size: 1470 125 476 278
Fragments arranged by size:
1470 476 278 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1967 2039 2349
Size: 1967 72 310
Fragments arranged by size:
1967 310 72
TatI w'GTAC_w
Cuts at: 0 527 1526 2349
Size: 527 999 823
Fragments arranged by size:
999 823 527
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 372 404 1249 1281 2349
Size: 372 32 845 32 1068
Fragments arranged by size:
1068 845 372 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1115 2349
Size: 1115 1234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 723 1431 2349
Size: 723 708 918
Fragments arranged by size:
918 723 708
XhoI C'TCGA_G
Cuts at: 0 2323 2349
Size: 2323 26
XmaI C'CCGG_G
Cuts at: 0 764 1187 2349
Size: 764 423 1162
Fragments arranged by size:
1162 764 423
Enzymes that do cut:
Acc65I AcuI AlfI AlwNI ApaI ApaLI ApoI AseI
AvaI BamHI BanI BanII BbsI BcgI BclI BglI
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI
BsrFI BsrGI BstAPI BstXI BstYI BtgI BtsI DrdI
EaeI EarI Eco57MI EcoO109I FspI HaeII Hin4I HincII
KpnI MmeI MscI MslI MspA1I NcoI NheI NspI
PasI PflMI PfoI PpuMI PspOMI PspXI PstI PvuII
SanDI SapI SbfI SexAI SfcI SmaI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AscI AsiSI AvrII BaeI BbeI
BbvCI BciVI BfrBI BglII BmgBI BpuEI BsaI BsaAI
BsaBI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspAI HindIII HpaI KasI
MfeI MluI NaeI NarI NdeI NgoMIV NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PsrI PvuI RsrII SacI SacII SalI ScaI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XmnI ZraI