(Linear) (Six-base) MAPSORT of: KIBB6305.seq Check: 5639 from: 1 to: 774 KIBB6305.orf With 182 enzymes: SgfI * June 26, 2008 10:46 .. AgeI A'CCGG_T Cuts at: 0 113 774 Size: 113 661 AleI CACnn'nnGTG Cuts at: 0 140 568 774 Size: 140 428 206 Fragments arranged by size: 428 206 140 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 575 607 774 Size: 575 32 167 Fragments arranged by size: 575 167 32 AlwNI CAG_nnn'CTG Cuts at: 0 431 486 774 Size: 431 55 288 Fragments arranged by size: 431 288 55 ApaI G_GGCC'C Cuts at: 0 625 774 Size: 625 149 ApoI r'AATT_y Cuts at: 0 81 774 Size: 81 693 BanII G_rGCy'C Cuts at: 0 550 625 774 Size: 550 75 149 Fragments arranged by size: 550 149 75 BbsI GAAGACnn'nnnn_ Cuts at: 0 211 711 774 Size: 211 500 63 Fragments arranged by size: 500 211 63 BglI GCCn_nnn'nGGC Cuts at: 0 415 774 Size: 415 359 Bme1580I G_kGCm'C Cuts at: 0 625 774 Size: 625 149 BmrI ACTGGGnnnn_n' Cuts at: 0 177 552 774 Size: 177 375 222 Fragments arranged by size: 375 222 177 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 91 140 245 513 687 774 Size: 91 49 105 268 174 87 Fragments arranged by size: 268 174 105 91 87 49 Bpu10I CC'TnA_GC Cuts at: 0 238 774 Size: 238 536 BsaI GGTCTCn'nnnn_ Cuts at: 0 221 735 774 Size: 221 514 39 Fragments arranged by size: 514 221 39 BsaAI yAC'GTr Cuts at: 0 267 774 Size: 267 507 BsaWI w'CCGG_w Cuts at: 0 36 113 774 Size: 36 77 661 Fragments arranged by size: 661 77 36 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 532 562 575 605 774 Size: 532 30 13 30 169 Fragments arranged by size: 532 169 30 30 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 525 774 Size: 525 249 BseYI C'CCAG_C Cuts at: 0 384 446 774 Size: 384 62 328 Fragments arranged by size: 384 328 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 774 Size: 295 479 Bsp1286I G_dGCh'C Cuts at: 0 550 625 774 Size: 550 75 149 Fragments arranged by size: 550 149 75 BspEI T'CCGG_A Cuts at: 0 36 774 Size: 36 738 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 509 774 Size: 509 265 BsrDI GCAATG_nn' Cuts at: 0 630 774 Size: 630 144 BsrFI r'CCGG_y Cuts at: 0 113 322 774 Size: 113 209 452 Fragments arranged by size: 452 209 113 BstXI CCAn_nnnn'nTGG Cuts at: 0 250 774 Size: 250 524 BtgI C'CryG_G Cuts at: 0 566 660 774 Size: 566 94 114 Fragments arranged by size: 566 114 94 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 318 747 774 Size: 318 429 27 Fragments arranged by size: 429 318 27 BtsI GCAGTG_nn' Cuts at: 0 186 774 Size: 186 588 DraIII CAC_nnn'GTG Cuts at: 0 192 774 Size: 192 582 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 91 140 245 513 687 774 Size: 91 49 105 268 174 87 Fragments arranged by size: 268 174 105 91 87 49 EcoNI CCTnn'n_nnAGG Cuts at: 0 490 774 Size: 490 284 EcoO109I rG'GnC_Cy Cuts at: 0 30 149 621 622 774 Size: 30 119 472 1 152 Fragments arranged by size: 472 152 119 30 1 EcoRI G'AATT_C Cuts at: 0 81 774 Size: 81 693 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 168 200 774 Size: 168 32 574 Fragments arranged by size: 574 168 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 39 774 Size: 39 735 MslI CAynn'nnrTG Cuts at: 0 140 568 774 Size: 140 428 206 Fragments arranged by size: 428 206 140 MspA1I CmG'CkG Cuts at: 0 181 384 474 774 Size: 181 203 90 300 Fragments arranged by size: 300 203 181 90 NaeI GCC'GGC Cuts at: 0 324 774 Size: 324 450 NcoI C'CATG_G Cuts at: 0 566 660 774 Size: 566 94 114 Fragments arranged by size: 566 114 94 NgoMIV G'CCGG_C Cuts at: 0 322 774 Size: 322 452 PflMI CCAn_nnn'nTGG Cuts at: 0 636 774 Size: 636 138 PfoI T'CCnGG_A Cuts at: 0 703 745 774 Size: 703 42 29 Fragments arranged by size: 703 42 29 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 575 607 774 Size: 575 32 167 Fragments arranged by size: 575 167 32 PpuMI rG'GwC_Cy Cuts at: 0 30 149 774 Size: 30 119 625 Fragments arranged by size: 625 119 30 PspOMI G'GGCC_C Cuts at: 0 621 774 Size: 621 153 PstI C_TGCA'G Cuts at: 0 491 774 Size: 491 283 PvuII CAG'CTG Cuts at: 0 181 384 474 774 Size: 181 203 90 300 Fragments arranged by size: 300 203 181 90 SanDI GG'GwC_CC Cuts at: 0 149 774 Size: 149 625 SfcI C'TryA_G Cuts at: 0 487 713 774 Size: 487 226 61 Fragments arranged by size: 487 226 61 StyI C'CwwG_G Cuts at: 0 566 660 774 Size: 566 94 114 Fragments arranged by size: 566 114 94 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 40 159 376 739 774 Size: 40 119 217 363 35 Fragments arranged by size: 363 217 119 40 35 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 115 471 613 774 Size: 115 356 142 161 Fragments arranged by size: 356 161 142 115 XmnI GAAnn'nnTTC Cuts at: 0 208 774 Size: 208 566 Enzymes that do cut: AgeI AleI AloI AlwNI ApaI ApoI BanII BbsI BglI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI Bsp1286I BspEI BspMI BsrDI BsrFI BstXI BtgI BtgZI BtsI DraIII Eco57MI EcoNI EcoO109I EcoRI Hin4I MmeI MslI MspA1I NaeI NcoI NgoMIV PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SanDI SfcI StyI TaqII XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AhdI AlfI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI NarI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI ZraI