Restriction map for pF1KB6305
 (Linear) (Six-base) MAPSORT of: KIBB6305.seq  Check: 5639  from: 1  to: 774



KIBB6305.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AgeI A'CCGG_T

Cuts at:      0     113     774 
   Size:        113     661
 
AleI CACnn'nnGTG

Cuts at:      0     140     568     774 
   Size:        140     428     206

  Fragments arranged by size: 

                 428     206     140
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     575     607     774 
   Size:        575      32     167

  Fragments arranged by size: 

                 575     167      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     431     486     774 
   Size:        431      55     288

  Fragments arranged by size: 

                 431     288      55
 
ApaI G_GGCC'C

Cuts at:      0     625     774 
   Size:        625     149
 
ApoI r'AATT_y

Cuts at:      0      81     774 
   Size:         81     693
 
BanII G_rGCy'C

Cuts at:      0     550     625     774 
   Size:        550      75     149

  Fragments arranged by size: 

                 550     149      75
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     211     711     774 
   Size:        211     500      63

  Fragments arranged by size: 

                 500     211      63
 
BglI GCCn_nnn'nGGC

Cuts at:      0     415     774 
   Size:        415     359
 
Bme1580I G_kGCm'C

Cuts at:      0     625     774 
   Size:        625     149
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     177     552     774 
   Size:        177     375     222

  Fragments arranged by size: 

                 375     222     177
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     140     245     513     687     774 
   Size:         91      49     105     268     174      87

  Fragments arranged by size: 

                 268     174     105      91      87      49
 
Bpu10I CC'TnA_GC

Cuts at:      0     238     774 
   Size:        238     536
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     221     735     774 
   Size:        221     514      39

  Fragments arranged by size: 

                 514     221      39
 
BsaAI yAC'GTr

Cuts at:      0     267     774 
   Size:        267     507
 
BsaWI w'CCGG_w

Cuts at:      0      36     113     774 
   Size:         36      77     661

  Fragments arranged by size: 

                 661      77      36
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     532     562     575     605     774 
   Size:        532      30      13      30     169

  Fragments arranged by size: 

                 532     169      30      30      13
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     525     774 
   Size:        525     249
 
BseYI C'CCAG_C

Cuts at:      0     384     446     774 
   Size:        384      62     328

  Fragments arranged by size: 

                 384     328      62
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     774 
   Size:        295     479
 
Bsp1286I G_dGCh'C

Cuts at:      0     550     625     774 
   Size:        550      75     149

  Fragments arranged by size: 

                 550     149      75
 
BspEI T'CCGG_A

Cuts at:      0      36     774 
   Size:         36     738
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     509     774 
   Size:        509     265
 
BsrDI GCAATG_nn'

Cuts at:      0     630     774 
   Size:        630     144
 
BsrFI r'CCGG_y

Cuts at:      0     113     322     774 
   Size:        113     209     452

  Fragments arranged by size: 

                 452     209     113
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     250     774 
   Size:        250     524
 
BtgI C'CryG_G

Cuts at:      0     566     660     774 
   Size:        566      94     114

  Fragments arranged by size: 

                 566     114      94
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     318     747     774 
   Size:        318     429      27

  Fragments arranged by size: 

                 429     318      27
 
BtsI GCAGTG_nn'

Cuts at:      0     186     774 
   Size:        186     588
 
DraIII CAC_nnn'GTG

Cuts at:      0     192     774 
   Size:        192     582
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     140     245     513     687     774 
   Size:         91      49     105     268     174      87

  Fragments arranged by size: 

                 268     174     105      91      87      49
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     490     774 
   Size:        490     284
 
EcoO109I rG'GnC_Cy

Cuts at:      0      30     149     621     622     774 
   Size:         30     119     472       1     152

  Fragments arranged by size: 

                 472     152     119      30       1
 
EcoRI G'AATT_C

Cuts at:      0      81     774 
   Size:         81     693
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     168     200     774 
   Size:        168      32     574

  Fragments arranged by size: 

                 574     168      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      39     774 
   Size:         39     735
 
MslI CAynn'nnrTG

Cuts at:      0     140     568     774 
   Size:        140     428     206

  Fragments arranged by size: 

                 428     206     140
 
MspA1I CmG'CkG

Cuts at:      0     181     384     474     774 
   Size:        181     203      90     300

  Fragments arranged by size: 

                 300     203     181      90
 
NaeI GCC'GGC

Cuts at:      0     324     774 
   Size:        324     450
 
NcoI C'CATG_G

Cuts at:      0     566     660     774 
   Size:        566      94     114

  Fragments arranged by size: 

                 566     114      94
 
NgoMIV G'CCGG_C

Cuts at:      0     322     774 
   Size:        322     452
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     636     774 
   Size:        636     138
 
PfoI T'CCnGG_A

Cuts at:      0     703     745     774 
   Size:        703      42      29

  Fragments arranged by size: 

                 703      42      29
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     575     607     774 
   Size:        575      32     167

  Fragments arranged by size: 

                 575     167      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      30     149     774 
   Size:         30     119     625

  Fragments arranged by size: 

                 625     119      30
 
PspOMI G'GGCC_C

Cuts at:      0     621     774 
   Size:        621     153
 
PstI C_TGCA'G

Cuts at:      0     491     774 
   Size:        491     283
 
PvuII CAG'CTG

Cuts at:      0     181     384     474     774 
   Size:        181     203      90     300

  Fragments arranged by size: 

                 300     203     181      90
 
SanDI GG'GwC_CC

Cuts at:      0     149     774 
   Size:        149     625
 
SfcI C'TryA_G

Cuts at:      0     487     713     774 
   Size:        487     226      61

  Fragments arranged by size: 

                 487     226      61
 
StyI C'CwwG_G

Cuts at:      0     566     660     774 
   Size:        566      94     114

  Fragments arranged by size: 

                 566     114      94
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      40     159     376     739     774 
   Size:         40     119     217     363      35

  Fragments arranged by size: 

                 363     217     119      40      35
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     115     471     613     774 
   Size:        115     356     142     161

  Fragments arranged by size: 

                 356     161     142     115
 
XmnI GAAnn'nnTTC

Cuts at:      0     208     774 
   Size:        208     566
 

 Enzymes that do cut:

     AgeI     AleI     AloI    AlwNI     ApaI     ApoI    BanII     BbsI
     BglI Bme1580I     BmrI     BpmI   Bpu10I     BsaI    BsaAI    BsaWI
    BsaXI    BseRI    BseYI     BsgI Bsp1286I    BspEI    BspMI    BsrDI
    BsrFI    BstXI     BtgI    BtgZI     BtsI   DraIII  Eco57MI    EcoNI
 EcoO109I    EcoRI    Hin4I     MmeI     MslI   MspA1I     NaeI     NcoI
   NgoMIV    PflMI     PfoI     PpiI    PpuMI   PspOMI     PstI    PvuII
    SanDI     SfcI     StyI    TaqII     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AhdI     AlfI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI    BpuEI
    BsaBI    BsaHI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI
    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     NarI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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