(Linear) (Six-base) MAPSORT of: KIBB6307.seq  Check: 4057  from: 1  to: 774



KIBB6307.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     366     572     774 
   Size:        366     206     202

  Fragments arranged by size: 

                 366     206     202
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      10      42     774 
   Size:         10      32     732

  Fragments arranged by size: 

                 732      32      10
 
AlwNI CAG_nnn'CTG

Cuts at:      0     550     774 
   Size:        550     224
 
ApoI r'AATT_y

Cuts at:      0     325     774 
   Size:        325     449
 
BanI G'GyrC_C

Cuts at:      0      45      54     130     429     774 
   Size:         45       9      76     299     345

  Fragments arranged by size: 

                 345     299      76      45       9
 
BanII G_rGCy'C

Cuts at:      0     397     774 
   Size:        397     377
 
BbeI G_GCGC'C

Cuts at:      0      49     774 
   Size:         49     725
 
BbvCI CC'TCA_GC

Cuts at:      0     337     774 
   Size:        337     437
 
BlpI GC'TnA_GC

Cuts at:      0     563     774 
   Size:        563     211
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     701     774 
   Size:        701      73
 
Bpu10I CC'TnA_GC

Cuts at:      0     337     774 
   Size:        337     437
 
BsaHI Gr'CG_yC

Cuts at:      0      46     146     774 
   Size:         46     100     628

  Fragments arranged by size: 

                 628     100      46
 
BsaWI w'CCGG_w

Cuts at:      0      51     774 
   Size:         51     723
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     241     271     774 
   Size:        241      30     503

  Fragments arranged by size: 

                 503     241      30
 
BsiEI CG_ry'CG

Cuts at:      0      36     774 
   Size:         36     738
 
BsmI GAATG_Cn'

Cuts at:      0     665     774 
   Size:        665     109
 
Bsp1286I G_dGCh'C

Cuts at:      0     397     774 
   Size:        397     377
 
BstYI r'GATC_y

Cuts at:      0     489     774 
   Size:        489     285
 
BtgI C'CryG_G

Cuts at:      0     260     774 
   Size:        260     514
 
BtsI GCAGTG_nn'

Cuts at:      0     308     774 
   Size:        308     466
 
EarI CTCTTCn'nnn_

Cuts at:      0     548     774 
   Size:        548     226
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     366     572     701     774 
   Size:        366     206     129      73

  Fragments arranged by size: 

                 366     206     129      73
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     634     774 
   Size:        634     140
 
EcoO109I rG'GnC_Cy

Cuts at:      0      18     774 
   Size:         18     756
 
HaeII r_GCGC'y

Cuts at:      0      49     774 
   Size:         49     725
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     241     273     284     316     488     520     774 
   Size:        241      32      11      32     172      32     254

  Fragments arranged by size: 

                 254     241     172      32      32      32      11
 
KasI G'GCGC_C

Cuts at:      0      45     774 
   Size:         45     729
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     321     456     467     503     774 
   Size:        321     135      11      36     271

  Fragments arranged by size: 

                 321     271     135      36      11
 
MspA1I CmG'CkG

Cuts at:      0      38     262     774 
   Size:         38     224     512

  Fragments arranged by size: 

                 512     224      38
 
NarI GG'CG_CC

Cuts at:      0      46     774 
   Size:         46     728
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     746     774 
   Size:        746      28
 
PpuMI rG'GwC_Cy

Cuts at:      0      18     774 
   Size:         18     756
 
SacII CC_GC'GG

Cuts at:      0     263     774 
   Size:        263     511
 
SanDI GG'GwC_CC

Cuts at:      0      18     774 
   Size:         18     756
 
ScaI AGT'ACT

Cuts at:      0     760     774 
   Size:        760      14
 
SfoI GGC'GCC

Cuts at:      0      47     774 
   Size:         47     727
 
StuI AGG'CCT

Cuts at:      0     342     774 
   Size:        342     432
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     417     774 
   Size:        417     357
 
TatI w'GTAC_w

Cuts at:      0     230     758     774 
   Size:        230     528      16

  Fragments arranged by size: 

                 528     230      16
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI     ApoI     BanI    BanII     BbeI    BbvCI
     BlpI     BpmI   Bpu10I    BsaHI    BsaWI    BsaXI    BsiEI     BsmI
 Bsp1286I    BstYI     BtgI     BtsI     EarI  Eco57MI    EcoNI EcoO109I
    HaeII    Hin4I     KasI     MmeI   MspA1I     NarI    PflMI    PpuMI
    SacII    SanDI     ScaI     SfoI     StuI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BseRI    BseYI     BsgI
  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MscI     MslI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI    SexAI
     SfcI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StyI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI