Restriction map for
pF1KB6309
(Linear) (Six-base) MAPSORT of: KIBB6309.seq Check: 8977 from: 1 to: 774
KIBB6309.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 472 774
Size: 472 302
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 774
Size: 655 119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 111 774
Size: 111 663
AlwNI CAG_nnn'CTG
Cuts at: 0 51 774
Size: 51 723
ApaI G_GGCC'C
Cuts at: 0 111 451 774
Size: 111 340 323
Fragments arranged by size:
340 323 111
AvaI C'yCGr_G
Cuts at: 0 15 36 343 774
Size: 15 21 307 431
Fragments arranged by size:
431 307 21 15
BanII G_rGCy'C
Cuts at: 0 111 451 774
Size: 111 340 323
Fragments arranged by size:
340 323 111
BbvCI CC'TCA_GC
Cuts at: 0 368 774
Size: 368 406
BclI T'GATC_A
Cuts at: 0 329 774
Size: 329 445
BglI GCCn_nnn'nGGC
Cuts at: 0 10 137 774
Size: 10 127 637
Fragments arranged by size:
637 127 10
BlpI GC'TnA_GC
Cuts at: 0 207 774
Size: 207 567
Bme1580I G_kGCm'C
Cuts at: 0 111 251 451 752 774
Size: 111 140 200 301 22
Fragments arranged by size:
301 200 140 111 22
BmtI G_CTAG'C
Cuts at: 0 67 163 206 774
Size: 67 96 43 568
Fragments arranged by size:
568 96 67 43
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 632 774
Size: 285 347 142
Fragments arranged by size:
347 285 142
Bpu10I CC'TnA_GC
Cuts at: 0 368 774
Size: 368 406
BsaAI yAC'GTr
Cuts at: 0 272 774
Size: 272 502
BsaWI w'CCGG_w
Cuts at: 0 284 774
Size: 284 490
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 373 774
Size: 373 401
BseYI C'CCAG_C
Cuts at: 0 51 80 222 774
Size: 51 29 142 552
Fragments arranged by size:
552 142 51 29
BsiEI CG_ry'CG
Cuts at: 0 731 774
Size: 731 43
BsiHKAI G_wGCw'C
Cuts at: 0 271 774
Size: 271 503
BsiWI C'GTAC_G
Cuts at: 0 272 774
Size: 272 502
BsmI GAATG_Cn'
Cuts at: 0 397 585 762 774
Size: 397 188 177 12
Fragments arranged by size:
397 188 177 12
BsmBI CGTCTCn'nnnn_
Cuts at: 0 156 774
Size: 156 618
Bsp1286I G_dGCh'C
Cuts at: 0 111 251 271 451 752 774
Size: 111 140 20 180 301 22
Fragments arranged by size:
301 180 140 111 22 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 437 472 774
Size: 437 35 302
Fragments arranged by size:
437 302 35
BsrBI CCG'CTC
Cuts at: 0 501 774
Size: 501 273
BsrFI r'CCGG_y
Cuts at: 0 9 774
Size: 9 765
BssHII G'CGCG_C
Cuts at: 0 84 211 774
Size: 84 127 563
Fragments arranged by size:
563 127 84
BssSI C'ACGA_G
Cuts at: 0 244 774
Size: 244 530
DraIII CAC_nnn'GTG
Cuts at: 0 553 774
Size: 553 221
EaeI y'GGCC_r
Cuts at: 0 2 728 774
Size: 2 726 46
Fragments arranged by size:
726 46 2
EagI C'GGCC_G
Cuts at: 0 728 774
Size: 728 46
EarI CTCTTCn'nnn_
Cuts at: 0 478 774
Size: 478 296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 632 655 774
Size: 285 347 23 119
Fragments arranged by size:
347 285 119 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 493 774
Size: 493 281
EcoO109I rG'GnC_Cy
Cuts at: 0 107 448 774
Size: 107 341 326
Fragments arranged by size:
341 326 107
HaeII r_GCGC'y
Cuts at: 0 64 243 774
Size: 64 179 531
Fragments arranged by size:
531 179 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 296 328 501 533 774
Size: 296 32 173 32 241
Fragments arranged by size:
296 241 173 32 32
MslI CAynn'nnrTG
Cuts at: 0 28 601 774
Size: 28 573 173
Fragments arranged by size:
573 173 28
MspA1I CmG'CkG
Cuts at: 0 72 628 669 774
Size: 72 556 41 105
Fragments arranged by size:
556 105 72 41
NaeI GCC'GGC
Cuts at: 0 11 774
Size: 11 763
NgoMIV G'CCGG_C
Cuts at: 0 9 774
Size: 9 765
NheI G'CTAG_C
Cuts at: 0 63 159 202 774
Size: 63 96 43 572
Fragments arranged by size:
572 96 63 43
NotI GC'GGCC_GC
Cuts at: 0 728 774
Size: 728 46
NspI r_CATG'y
Cuts at: 0 95 774
Size: 95 679
PflMI CCAn_nnn'nTGG
Cuts at: 0 51 774
Size: 51 723
PspOMI G'GGCC_C
Cuts at: 0 107 447 774
Size: 107 340 327
Fragments arranged by size:
340 327 107
PvuII CAG'CTG
Cuts at: 0 72 628 774
Size: 72 556 146
Fragments arranged by size:
556 146 72
SfcI C'TryA_G
Cuts at: 0 493 774
Size: 493 281
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 10 774
Size: 10 764
SmaI CCC'GGG
Cuts at: 0 17 774
Size: 17 757
SmlI C'TyrA_G
Cuts at: 0 36 774
Size: 36 738
SphI G_CATG'C
Cuts at: 0 95 774
Size: 95 679
StyI C'CwwG_G
Cuts at: 0 402 774
Size: 402 372
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 43 774
Size: 43 731
XhoI C'TCGA_G
Cuts at: 0 36 774
Size: 36 738
XmaI C'CCGG_G
Cuts at: 0 15 774
Size: 15 759
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaI AvaI BanII BbvCI
BclI BglI BlpI Bme1580I BmtI BpmI Bpu10I BsaAI
BsaWI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrFI BssHII BssSI DraIII EaeI
EagI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I MslI
MspA1I NaeI NgoMIV NheI NotI NspI PflMI PspOMI
PvuII SfcI SfiI SmaI SmlI SphI StyI TaqII
XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI
BfrBI BglII BmgBI BmrI BplI BpuEI BsaI BsaBI
BsaHI BsaXI BsgI BspEI BspHI BsrDI BsrGI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NarI NcoI
NdeI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XcmI
XmnI ZraI