(Linear) (Six-base) MAPSORT of: KIBB6315.seq  Check: 7179  from: 1  to: 777



KIBB6315.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     639     777 
   Size:        639     138
 
AflIII A'CryG_T

Cuts at:      0     677     777 
   Size:        677     100
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     357     777 
   Size:        357     420
 
ApoI r'AATT_y

Cuts at:      0     571     592     733     777 
   Size:        571      21     141      44

  Fragments arranged by size: 

                 571     141      44      21
 
BglII A'GATC_T

Cuts at:      0     519     777 
   Size:        519     258
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     777 
   Size:        209     568
 
BseYI C'CCAG_C

Cuts at:      0     754     777 
   Size:        754      23
 
BsrDI GCAATG_nn'

Cuts at:      0     533     777 
   Size:        533     244
 
BsrGI T'GTAC_A

Cuts at:      0     203     777 
   Size:        203     574
 
BstYI r'GATC_y

Cuts at:      0     519     777 
   Size:        519     258
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0       2     777 
   Size:          2     775
 
DraI TTT'AAA

Cuts at:      0     570     777 
   Size:        570     207
 
EaeI y'GGCC_r

Cuts at:      0     530     777 
   Size:        530     247
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     639     777 
   Size:        639     138
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     664     696     777 
   Size:        664      32      81

  Fragments arranged by size: 

                 664      81      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     581     777 
   Size:        581     196
 
MscI TGG'CCA

Cuts at:      0     532     777 
   Size:        532     245
 
MslI CAynn'nnrTG

Cuts at:      0     301     777 
   Size:        301     476
 
MspA1I CmG'CkG

Cuts at:      0     201     777 
   Size:        201     576
 
NspI r_CATG'y

Cuts at:      0     681     777 
   Size:        681      96
 
PciI A'CATG_T

Cuts at:      0     677     777 
   Size:        677     100
 
PfoI T'CCnGG_A

Cuts at:      0     501     777 
   Size:        501     276
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     137     169     777 
   Size:        137      32     608

  Fragments arranged by size: 

                 608     137      32
 
PvuII CAG'CTG

Cuts at:      0     201     777 
   Size:        201     576
 
SmlI C'TyrA_G

Cuts at:      0     224     777 
   Size:        224     553
 
SwaI ATTT'AAAT

Cuts at:      0     570     777 
   Size:        570     207
 
TatI w'GTAC_w

Cuts at:      0     203     766     777 
   Size:        203     563      11

  Fragments arranged by size: 

                 563     203      11
 
XbaI T'CTAG_A

Cuts at:      0     522     777 
   Size:        522     255
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     632     777 
   Size:        632     145
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI     ApoI    BglII    BpuEI    BseYI    BsrDI
    BsrGI    BstYI    BtgZI     DraI     EaeI  Eco57MI    Hin4I     MmeI
     MscI     MslI   MspA1I     NspI     PciI     PfoI     PsrI    PvuII
     SmlI     SwaI     TatI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
    TaqII     TstI  Tth111I     XhoI     XmaI     XmnI     ZraI