Restriction map for
pF1KB6320
(Linear) (Six-base) MAPSORT of: KIBB6320.seq Check: 9097 from: 1 to: 846
KIBB6320.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
Acc65I G'GTAC_C
Cuts at: 0 717 846
Size: 717 129
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 846
Size: 166 680
AleI CACnn'nnGTG
Cuts at: 0 301 394 846
Size: 301 93 452
Fragments arranged by size:
452 301 93
AlwNI CAG_nnn'CTG
Cuts at: 0 120 846
Size: 120 726
AvaI C'yCGr_G
Cuts at: 0 10 423 721 846
Size: 10 413 298 125
Fragments arranged by size:
413 298 125 10
BanI G'GyrC_C
Cuts at: 0 295 447 717 846
Size: 295 152 270 129
Fragments arranged by size:
295 270 152 129
BanII G_rGCy'C
Cuts at: 0 665 846
Size: 665 181
BbeI G_GCGC'C
Cuts at: 0 451 846
Size: 451 395
BciVI GTATCCnnnnn_n'
Cuts at: 0 259 846
Size: 259 587
BglII A'GATC_T
Cuts at: 0 827 846
Size: 827 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 99 846
Size: 99 747
BsaI GGTCTCn'nnnn_
Cuts at: 0 7 343 646 846
Size: 7 336 303 200
Fragments arranged by size:
336 303 200 7
BsaHI Gr'CG_yC
Cuts at: 0 448 846
Size: 448 398
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 664 846
Size: 664 182
BseYI C'CCAG_C
Cuts at: 0 593 846
Size: 593 253
BsiHKAI G_wGCw'C
Cuts at: 0 665 846
Size: 665 181
Bsp1286I G_dGCh'C
Cuts at: 0 665 846
Size: 665 181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 650 846
Size: 650 196
BsrBI CCG'CTC
Cuts at: 0 309 846
Size: 309 537
BsrDI GCAATG_nn'
Cuts at: 0 278 846
Size: 278 568
BstAPI GCAn_nnn'nTGC
Cuts at: 0 468 570 846
Size: 468 102 276
Fragments arranged by size:
468 276 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 396 846
Size: 396 450
BstYI r'GATC_y
Cuts at: 0 116 536 827 846
Size: 116 420 291 19
Fragments arranged by size:
420 291 116 19
BtgI C'CryG_G
Cuts at: 0 395 846
Size: 395 451
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 720 797 846
Size: 720 77 49
Fragments arranged by size:
720 77 49
EaeI y'GGCC_r
Cuts at: 0 111 356 531 846
Size: 111 245 175 315
Fragments arranged by size:
315 245 175 111
EarI CTCTTCn'nnn_
Cuts at: 0 322 846
Size: 322 524
EcoICRI GAG'CTC
Cuts at: 0 663 846
Size: 663 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 846
Size: 166 680
EcoNI CCTnn'n_nnAGG
Cuts at: 0 646 846
Size: 646 200
EcoO109I rG'GnC_Cy
Cuts at: 0 415 846
Size: 415 431
EcoRV GAT'ATC
Cuts at: 0 126 276 846
Size: 126 150 570
Fragments arranged by size:
570 150 126
HaeII r_GCGC'y
Cuts at: 0 194 451 846
Size: 194 257 395
Fragments arranged by size:
395 257 194
KasI G'GCGC_C
Cuts at: 0 447 846
Size: 447 399
KpnI G_GTAC'C
Cuts at: 0 721 846
Size: 721 125
MscI TGG'CCA
Cuts at: 0 113 533 846
Size: 113 420 313
Fragments arranged by size:
420 313 113
MslI CAynn'nnrTG
Cuts at: 0 301 394 846
Size: 301 93 452
Fragments arranged by size:
452 301 93
NarI GG'CG_CC
Cuts at: 0 448 846
Size: 448 398
NruI TCG'CGA
Cuts at: 0 782 846
Size: 782 64
NspI r_CATG'y
Cuts at: 0 432 471 492 846
Size: 432 39 21 354
Fragments arranged by size:
432 354 39 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 120 846
Size: 120 726
PfoI T'CCnGG_A
Cuts at: 0 119 846
Size: 119 727
PstI C_TGCA'G
Cuts at: 0 794 846
Size: 794 52
SacI G_AGCT'C
Cuts at: 0 665 846
Size: 665 181
SbfI CC_TGCA'GG
Cuts at: 0 794 846
Size: 794 52
SfcI C'TryA_G
Cuts at: 0 790 846
Size: 790 56
SfoI GGC'GCC
Cuts at: 0 449 846
Size: 449 397
SmaI CCC'GGG
Cuts at: 0 425 723 846
Size: 425 298 123
Fragments arranged by size:
425 298 123
SphI G_CATG'C
Cuts at: 0 432 846
Size: 432 414
StuI AGG'CCT
Cuts at: 0 163 521 846
Size: 163 358 325
Fragments arranged by size:
358 325 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 280 846
Size: 280 566
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 396 846
Size: 396 450
XmaI C'CCGG_G
Cuts at: 0 423 721 846
Size: 423 298 125
Fragments arranged by size:
423 298 125
Enzymes that do cut:
Acc65I AcuI AleI AlwNI AvaI BanI BanII BbeI
BciVI BglII BmrI BsaI BsaHI BseRI BseYI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BstAPI BstXI BstYI BtgI
BtgZI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
HaeII KasI KpnI MscI MslI NarI NruI NspI
PflMI PfoI PstI SacI SbfI SfcI SfoI SmaI
SphI StuI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoRI FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI MfeI MluI
MmeI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SapI ScaI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI StyI SwaI
TatI TstI Tth111I XbaI XhoI XmnI ZraI