(Linear) (Six-base) MAPSORT of: KIBB6321.seq  Check: 3183  from: 1  to: 783



KIBB6321.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AfeI AGC'GCT

Cuts at:      0     338     504     783 
   Size:        338     166     279

  Fragments arranged by size: 

                 338     279     166
 
ApoI r'AATT_y

Cuts at:      0     107     191     783 
   Size:        107      84     592

  Fragments arranged by size: 

                 592     107      84
 
BanI G'GyrC_C

Cuts at:      0      94     783 
   Size:         94     689
 
BclI T'GATC_A

Cuts at:      0     707     783 
   Size:        707      76
 
BfrBI ATG'CAT

Cuts at:      0     488     783 
   Size:        488     295
 
BlpI GC'TnA_GC

Cuts at:      0     740     783 
   Size:        740      43
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       3      23     783 
   Size:          3      20     760

  Fragments arranged by size: 

                 760      20       3
 
BseYI C'CCAG_C

Cuts at:      0     413     783 
   Size:        413     370
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     138     783 
   Size:        138     645
 
BsrDI GCAATG_nn'

Cuts at:      0     275     397     783 
   Size:        275     122     386

  Fragments arranged by size: 

                 386     275     122
 
BsrGI T'GTAC_A

Cuts at:      0     404     783 
   Size:        404     379
 
BssSI C'ACGA_G

Cuts at:      0     718     783 
   Size:        718      65
 
BtgI C'CryG_G

Cuts at:      0      71     599     783 
   Size:         71     528     184

  Fragments arranged by size: 

                 528     184      71
 
BtsI GCAGTG_nn'

Cuts at:      0     228     783 
   Size:        228     555
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     471     506     783 
   Size:        471      35     277

  Fragments arranged by size: 

                 471     277      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     558     783 
   Size:        558     225
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       3      23     783 
   Size:          3      20     760

  Fragments arranged by size: 

                 760      20       3
 
EcoRV GAT'ATC

Cuts at:      0     348     783 
   Size:        348     435
 
HaeII r_GCGC'y

Cuts at:      0     340     506     783 
   Size:        340     166     277

  Fragments arranged by size: 

                 340     277     166
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     137     169     783 
   Size:        137      32     614

  Fragments arranged by size: 

                 614     137      32
 
HindIII A'AGCT_T

Cuts at:      0     160     356     783 
   Size:        160     196     427

  Fragments arranged by size: 

                 427     196     160
 
MspA1I CmG'CkG

Cuts at:      0     511     783 
   Size:        511     272
 
NcoI C'CATG_G

Cuts at:      0      71     599     783 
   Size:         71     528     184

  Fragments arranged by size: 

                 528     184      71
 
NsiI A_TGCA'T

Cuts at:      0     490     783 
   Size:        490     293
 
NspI r_CATG'y

Cuts at:      0      91     488     783 
   Size:         91     397     295

  Fragments arranged by size: 

                 397     295      91
 
PfoI T'CCnGG_A

Cuts at:      0      19     783 
   Size:         19     764
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     257     289     783 
   Size:        257      32     494

  Fragments arranged by size: 

                 494     257      32
 
PsiI TTA'TAA

Cuts at:      0     197     783 
   Size:        197     586
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     662     694     783 
   Size:        662      32      89

  Fragments arranged by size: 

                 662      89      32
 
PstI C_TGCA'G

Cuts at:      0     510     783 
   Size:        510     273
 
PvuII CAG'CTG

Cuts at:      0     511     783 
   Size:        511     272
 
ScaI AGT'ACT

Cuts at:      0     587     783 
   Size:        587     196
 
SfcI C'TryA_G

Cuts at:      0     506     783 
   Size:        506     277
 
StyI C'CwwG_G

Cuts at:      0      71     599     783 
   Size:         71     528     184

  Fragments arranged by size: 

                 528     184      71
 
TatI w'GTAC_w

Cuts at:      0     404     585     783 
   Size:        404     181     198

  Fragments arranged by size: 

                 404     198     181
 

 Enzymes that do cut:

     AfeI     ApoI     BanI     BclI    BfrBI     BlpI     BpmI    BseYI
    BsmBI    BsrDI    BsrGI    BssSI     BtgI     BtsI    CspCI     DrdI
  Eco57MI    EcoRV    HaeII    Hin4I  HindIII   MspA1I     NcoI     NsiI
     NspI     PfoI     PpiI     PsiI     PsrI     PstI    PvuII     ScaI
     SfcI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BglI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     ClaI     DraI   DraIII     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    FspAI
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PasI     PciI    PflMI     PmeI     PmlI    PpuMI    PshAI
   PspOMI    PspXI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI