(Linear) (Six-base) MAPSORT of: KIBB6328.seq  Check: 1118  from: 1  to: 693



KIBB6328.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      39     114     693 
   Size:         39      75     579

  Fragments arranged by size: 

                 579      75      39
 
AflII C'TTAA_G

Cuts at:      0     244     693 
   Size:        244     449
 
AlwNI CAG_nnn'CTG

Cuts at:      0     184     693 
   Size:        184     509
 
AvrII C'CTAG_G

Cuts at:      0     601     693 
   Size:        601      92
 
BanII G_rGCy'C

Cuts at:      0     340     693 
   Size:        340     353
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      65     260     693 
   Size:         65     195     433

  Fragments arranged by size: 

                 433     195      65
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     342     693 
   Size:        342     351
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     614     693 
   Size:        614      79
 
BsaWI w'CCGG_w

Cuts at:      0     683     693 
   Size:        683      10
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     322     352     693 
   Size:        322      30     341

  Fragments arranged by size: 

                 341     322      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      80     541     693 
   Size:         80     461     152

  Fragments arranged by size: 

                 461     152      80
 
BseYI C'CCAG_C

Cuts at:      0     461     693 
   Size:        461     232
 
Bsp1286I G_dGCh'C

Cuts at:      0     340     693 
   Size:        340     353
 
BstYI r'GATC_y

Cuts at:      0     272     693 
   Size:        272     421
 
DraIII CAC_nnn'GTG

Cuts at:      0     202     693 
   Size:        202     491
 
EarI CTCTTCn'nnn_

Cuts at:      0      22     693 
   Size:         22     671
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      39     114     614     693 
   Size:         39      75     500      79

  Fragments arranged by size: 

                 500      79      75      39
 
EcoO109I rG'GnC_Cy

Cuts at:      0     143     181     693 
   Size:        143      38     512

  Fragments arranged by size: 

                 512     143      38
 
HaeII r_GCGC'y

Cuts at:      0     534     693 
   Size:        534     159
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     322     354     601     633     693 
   Size:        322      32     247      32      60

  Fragments arranged by size: 

                 322     247      60      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     490     693 
   Size:        490     203
 
PfoI T'CCnGG_A

Cuts at:      0      39     656     693 
   Size:         39     617      37

  Fragments arranged by size: 

                 617      39      37
 
PpuMI rG'GwC_Cy

Cuts at:      0     181     693 
   Size:        181     512
 
SexAI A'CCwGG_T

Cuts at:      0     198     693 
   Size:        198     495
 
SfcI C'TryA_G

Cuts at:      0     454     569     693 
   Size:        454     115     124

  Fragments arranged by size: 

                 454     124     115
 
SmlI C'TyrA_G

Cuts at:      0     244     693 
   Size:        244     449
 
StyI C'CwwG_G

Cuts at:      0     601     693 
   Size:        601      92
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     329     439     693 
   Size:        329     110     254

  Fragments arranged by size: 

                 329     254     110
 

 Enzymes that do cut:

     AcuI    AflII    AlwNI    AvrII    BanII    BciVI     BmrI     BpmI
    BsaWI    BsaXI    BseRI    BseYI Bsp1286I    BstYI   DraIII     EarI
  Eco57MI EcoO109I    HaeII    Hin4I     MslI     PfoI    PpuMI    SexAI
     SfcI     SmlI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI