Restriction map for
pF1KB6329
(Linear) (Six-base) MAPSORT of: KIBB6329.seq Check: 4391 from: 1 to: 783
KIBB6329.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 633 783
Size: 633 150
AccI GT'mk_AC
Cuts at: 0 380 783
Size: 380 403
AflIII A'CryG_T
Cuts at: 0 383 746 783
Size: 383 363 37
Fragments arranged by size:
383 363 37
AgeI A'CCGG_T
Cuts at: 0 660 783
Size: 660 123
AhdI GACnn_n'nnGTC
Cuts at: 0 75 783
Size: 75 708
AlwNI CAG_nnn'CTG
Cuts at: 0 487 783
Size: 487 296
ApaI G_GGCC'C
Cuts at: 0 52 522 783
Size: 52 470 261
Fragments arranged by size:
470 261 52
ApaLI G'TGCA_C
Cuts at: 0 304 783
Size: 304 479
AvaI C'yCGr_G
Cuts at: 0 44 783
Size: 44 739
BanI G'GyrC_C
Cuts at: 0 177 202 783
Size: 177 25 581
Fragments arranged by size:
581 177 25
BanII G_rGCy'C
Cuts at: 0 52 434 522 701 783
Size: 52 382 88 179 82
Fragments arranged by size:
382 179 88 82 52
BbeI G_GCGC'C
Cuts at: 0 181 206 783
Size: 181 25 577
Fragments arranged by size:
577 181 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 537 630 783
Size: 537 93 153
Fragments arranged by size:
537 153 93
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 521 555 783
Size: 521 34 228
Fragments arranged by size:
521 228 34
BglI GCCn_nnn'nGGC
Cuts at: 0 517 783
Size: 517 266
BglII A'GATC_T
Cuts at: 0 269 783
Size: 269 514
Bme1580I G_kGCm'C
Cuts at: 0 52 308 522 783
Size: 52 256 214 261
Fragments arranged by size:
261 256 214 52
BmgBI CAC'GTC
Cuts at: 0 747 783
Size: 747 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 217 475 783
Size: 217 258 308
Fragments arranged by size:
308 258 217
BmtI G_CTAG'C
Cuts at: 0 461 783
Size: 461 322
Bpu10I CC'TnA_GC
Cuts at: 0 212 574 760 783
Size: 212 362 186 23
Fragments arranged by size:
362 212 186 23
BsaI GGTCTCn'nnnn_
Cuts at: 0 289 584 783
Size: 289 295 199
Fragments arranged by size:
295 289 199
BsaAI yAC'GTr
Cuts at: 0 384 564 783
Size: 384 180 219
Fragments arranged by size:
384 219 180
BsaHI Gr'CG_yC
Cuts at: 0 178 203 783
Size: 178 25 580
Fragments arranged by size:
580 178 25
BsaWI w'CCGG_w
Cuts at: 0 660 783
Size: 660 123
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 208 700 783
Size: 52 156 492 83
Fragments arranged by size:
492 156 83 52
BsiEI CG_ry'CG
Cuts at: 0 14 783
Size: 14 769
BsiHKAI G_wGCw'C
Cuts at: 0 308 434 608 701 761 783
Size: 308 126 174 93 60 22
Fragments arranged by size:
308 174 126 93 60 22
Bsp1286I G_dGCh'C
Cuts at: 0 52 308 434 522 608 701 761 783
Size: 52 256 126 88 86 93 60 22
Fragments arranged by size:
256 126 93 88 86 60 52 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 242 500 602 633 783
Size: 242 258 102 31 150
Fragments arranged by size:
258 242 150 102 31
BsrFI r'CCGG_y
Cuts at: 0 578 660 783
Size: 578 82 123
Fragments arranged by size:
578 123 82
BssSI C'ACGA_G
Cuts at: 0 669 783
Size: 669 114
BstEII G'GTnAC_C
Cuts at: 0 67 783
Size: 67 716
BstYI r'GATC_y
Cuts at: 0 269 783
Size: 269 514
Bsu36I CC'TnA_GG
Cuts at: 0 242 783
Size: 242 541
BtgI C'CryG_G
Cuts at: 0 769 783
Size: 769 14
BtsI GCAGTG_nn'
Cuts at: 0 452 783
Size: 452 331
EaeI y'GGCC_r
Cuts at: 0 11 772 783
Size: 11 761 11
Fragments arranged by size:
761 11 11
EagI C'GGCC_G
Cuts at: 0 11 783
Size: 11 772
EarI CTCTTCn'nnn_
Cuts at: 0 130 783
Size: 130 653
EcoICRI GAG'CTC
Cuts at: 0 432 699 783
Size: 432 267 84
Fragments arranged by size:
432 267 84
EcoO109I rG'GnC_Cy
Cuts at: 0 48 156 509 570 783
Size: 48 108 353 61 213
Fragments arranged by size:
353 213 108 61 48
HaeII r_GCGC'y
Cuts at: 0 181 206 783
Size: 181 25 577
Fragments arranged by size:
577 181 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 61 93 736 768 783
Size: 61 32 643 32 15
Fragments arranged by size:
643 61 32 32 15
KasI G'GCGC_C
Cuts at: 0 177 202 783
Size: 177 25 581
Fragments arranged by size:
581 177 25
MslI CAynn'nnrTG
Cuts at: 0 349 404 783
Size: 349 55 379
Fragments arranged by size:
379 349 55
MspA1I CmG'CkG
Cuts at: 0 54 173 783
Size: 54 119 610
Fragments arranged by size:
610 119 54
NarI GG'CG_CC
Cuts at: 0 178 203 783
Size: 178 25 580
Fragments arranged by size:
580 178 25
NheI G'CTAG_C
Cuts at: 0 457 783
Size: 457 326
PfoI T'CCnGG_A
Cuts at: 0 611 783
Size: 611 172
PpuMI rG'GwC_Cy
Cuts at: 0 570 783
Size: 570 213
PshAI GACnn'nnGTC
Cuts at: 0 223 783
Size: 223 560
PspOMI G'GGCC_C
Cuts at: 0 48 518 783
Size: 48 470 265
Fragments arranged by size:
470 265 48
PstI C_TGCA'G
Cuts at: 0 419 783
Size: 419 364
SacI G_AGCT'C
Cuts at: 0 434 701 783
Size: 434 267 82
Fragments arranged by size:
434 267 82
SexAI A'CCwGG_T
Cuts at: 0 374 783
Size: 374 409
SfcI C'TryA_G
Cuts at: 0 415 783
Size: 415 368
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 517 783
Size: 517 266
SfoI GGC'GCC
Cuts at: 0 179 204 783
Size: 179 25 579
Fragments arranged by size:
579 179 25
StyI C'CwwG_G
Cuts at: 0 248 783
Size: 248 535
TatI w'GTAC_w
Cuts at: 0 749 783
Size: 749 34
Enzymes that do cut:
AarI AccI AflIII AgeI AhdI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BbsI BcgI BglI BglII
Bme1580I BmgBI BmrI BmtI Bpu10I BsaI BsaAI BsaHI
BsaWI BseRI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BssSI
BstEII BstYI Bsu36I BtgI BtsI EaeI EagI EarI
EcoICRI EcoO109I HaeII Hin4I KasI MslI MspA1I NarI
NheI PfoI PpuMI PshAI PspOMI PstI SacI SexAI
SfcI SfiI SfoI StyI TatI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AleI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BciVI BclI BfrBI BlpI BplI BpmI BpuEI
BsaBI BsaXI BseYI BsgI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstXI
BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EciI
Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
NaeI NcoI NdeI NgoMIV NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI