(Linear) (Six-base) MAPSORT of: KIBB6334.seq  Check: 7620  from: 1  to: 867



KIBB6334.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     304     867 
   Size:         43     261     563

  Fragments arranged by size: 

                 563     261      43
 
AflIII A'CryG_T

Cuts at:      0     153     638     867 
   Size:        153     485     229

  Fragments arranged by size: 

                 485     229     153
 
BglII A'GATC_T

Cuts at:      0     143     867 
   Size:        143     724
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     514     631     867 
   Size:         34     480     117     236

  Fragments arranged by size: 

                 480     236     117      34
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     395     867 
   Size:        395     472
 
BsaAI yAC'GTr

Cuts at:      0     726     867 
   Size:        726     141
 
BsaBI GATnn'nnATC

Cuts at:      0      73     796     867 
   Size:         73     723      71

  Fragments arranged by size: 

                 723      73      71
 
BsaWI w'CCGG_w

Cuts at:      0     272     867 
   Size:        272     595
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     487     517     867 
   Size:        487      30     350

  Fragments arranged by size: 

                 487     350      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     175     502     511     867 
   Size:        175     327       9     356

  Fragments arranged by size: 

                 356     327     175       9
 
BseYI C'CCAG_C

Cuts at:      0     353     822     867 
   Size:        353     469      45

  Fragments arranged by size: 

                 469     353      45
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     179     867 
   Size:        179     688
 
BspEI T'CCGG_A

Cuts at:      0     272     867 
   Size:        272     595
 
BssSI C'ACGA_G

Cuts at:      0     737     867 
   Size:        737     130
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     663     867 
   Size:        663     204
 
BstYI r'GATC_y

Cuts at:      0     143     867 
   Size:        143     724
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     636     867 
   Size:        636     231
 
DraIII CAC_nnn'GTG

Cuts at:      0     717     867 
   Size:        717     150
 
EaeI y'GGCC_r

Cuts at:      0     521     867 
   Size:        521     346
 
EarI CTCTTCn'nnn_

Cuts at:      0     280     867 
   Size:        280     587
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34      43     304     514     631     867 
   Size:         34       9     261     210     117     236

  Fragments arranged by size: 

                 261     236     210     117      34       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     130     162     307     339     867 
   Size:        130      32     145      32     528

  Fragments arranged by size: 

                 528     145     130      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     485     867 
   Size:        485     382
 
MslI CAynn'nnrTG

Cuts at:      0     643     655     661     867 
   Size:        643      12       6     206

  Fragments arranged by size: 

                 643     206      12       6
 
MspA1I CmG'CkG

Cuts at:      0      21      36     450     867 
   Size:         21      15     414     417

  Fragments arranged by size: 

                 417     414      21      15
 
NspI r_CATG'y

Cuts at:      0     157     642     867 
   Size:        157     485     225

  Fragments arranged by size: 

                 485     225     157
 
PciI A'CATG_T

Cuts at:      0     153     638     867 
   Size:        153     485     229

  Fragments arranged by size: 

                 485     229     153
 
PmlI CAC'GTG

Cuts at:      0     726     867 
   Size:        726     141
 
PvuII CAG'CTG

Cuts at:      0      21      36     867 
   Size:         21      15     831

  Fragments arranged by size: 

                 831      21      15
 
SapI GCTCTTCn'nnn_

Cuts at:      0     280     867 
   Size:        280     587
 
SfcI C'TryA_G

Cuts at:      0     177     867 
   Size:        177     690
 
SmlI C'TyrA_G

Cuts at:      0     410     867 
   Size:        410     457
 
StyI C'CwwG_G

Cuts at:      0     218     867 
   Size:        218     649
 

 Enzymes that do cut:

     AcuI   AflIII    BglII     BpmI    BpuEI    BsaAI    BsaBI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BspEI    BssSI    BstXI    BstYI
    BtgZI   DraIII     EaeI     EarI  Eco57MI    Hin4I     MmeI     MslI
   MspA1I     NspI     PciI     PmlI    PvuII     SapI     SfcI     SmlI
     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
     BsaI    BsaHI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI
     DraI     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI