(Linear) (Six-base) MAPSORT of: KIBB6336.seq  Check: 3910  from: 1  to: 870



KIBB6336.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     625     870 
   Size:        625     245
 
AclI AA'CG_TT

Cuts at:      0     543     870 
   Size:        543     327
 
AlwNI CAG_nnn'CTG

Cuts at:      0     640     870 
   Size:        640     230
 
ApoI r'AATT_y

Cuts at:      0     276     379     463     870 
   Size:        276     103      84     407

  Fragments arranged by size: 

                 407     276     103      84
 
BsaBI GATnn'nnATC

Cuts at:      0     428     870 
   Size:        428     442
 
BsaWI w'CCGG_w

Cuts at:      0      69     870 
   Size:         69     801
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      88     205     775     862     870 
   Size:         88     117     570      87       8

  Fragments arranged by size: 

                 570     117      88      87       8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     302     870 
   Size:        302     568
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     625     870 
   Size:        625     245
 
BstBI TT'CG_AA

Cuts at:      0     496     870 
   Size:        496     374
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     281     870 
   Size:        281     589
 
BtsI GCAGTG_nn'

Cuts at:      0     319     346     870 
   Size:        319      27     524

  Fragments arranged by size: 

                 524     319      27
 
EaeI y'GGCC_r

Cuts at:      0     676     870 
   Size:        676     194
 
EarI CTCTTCn'nnn_

Cuts at:      0     241     335     571     752     870 
   Size:        241      94     236     181     118

  Fragments arranged by size: 

                 241     236     181     118      94
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     217     870 
   Size:        217     653
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      64      96     379     411     471     503     644     676 
   Size:         64      32     283      32      60      32     141      32

Cuts at:    676     870 
   Size:        194

  Fragments arranged by size: 

                 283     194     141      64      60      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     300     870 
   Size:        300     570
 
MscI TGG'CCA

Cuts at:      0     678     870 
   Size:        678     192
 
MslI CAynn'nnrTG

Cuts at:      0     143     473     629     870 
   Size:        143     330     156     241

  Fragments arranged by size: 

                 330     241     156     143
 
PstI C_TGCA'G

Cuts at:      0      93     870 
   Size:         93     777
 
ScaI AGT'ACT

Cuts at:      0     694     870 
   Size:        694     176
 
SexAI A'CCwGG_T

Cuts at:      0     610     870 
   Size:        610     260
 
SfcI C'TryA_G

Cuts at:      0      89     870 
   Size:         89     781
 
TatI w'GTAC_w

Cuts at:      0     150     692     870 
   Size:        150     542     178

  Fragments arranged by size: 

                 542     178     150
 

 Enzymes that do cut:

     AarI     AclI    AlwNI     ApoI    BsaBI    BsaWI    BseRI     BsgI
    BspMI    BstBI    BstXI     BtsI     EaeI     EarI     EciI    Hin4I
   HincII     MscI     MslI     PstI     ScaI    SexAI     SfcI     TatI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI