Restriction map for
pF1KB6340
(Linear) (Six-base) MAPSORT of: KIBB6340.seq Check: 4183 from: 1 to: 870
KIBB6340.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 870
Size: 244 626
AlwNI CAG_nnn'CTG
Cuts at: 0 567 613 753 870
Size: 567 46 140 117
Fragments arranged by size:
567 140 117 46
ApaI G_GGCC'C
Cuts at: 0 745 870
Size: 745 125
AseI AT'TA_AT
Cuts at: 0 835 870
Size: 835 35
AvaI C'yCGr_G
Cuts at: 0 592 870
Size: 592 278
BamHI G'GATC_C
Cuts at: 0 557 870
Size: 557 313
BanI G'GyrC_C
Cuts at: 0 861 870
Size: 861 9
BanII G_rGCy'C
Cuts at: 0 104 422 517 696 745 870
Size: 104 318 95 179 49 125
Fragments arranged by size:
318 179 125 104 95 49
BbvCI CC'TCA_GC
Cuts at: 0 149 870
Size: 149 721
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 535 569 870
Size: 535 34 301
Fragments arranged by size:
535 301 34
BclI T'GATC_A
Cuts at: 0 425 870
Size: 425 445
BlpI GC'TnA_GC
Cuts at: 0 31 870
Size: 31 839
Bme1580I G_kGCm'C
Cuts at: 0 745 870
Size: 745 125
BmgBI CAC'GTC
Cuts at: 0 240 870
Size: 240 630
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 88 730 870
Size: 46 42 642 140
Fragments arranged by size:
642 140 46 42
Bpu10I CC'TnA_GC
Cuts at: 0 149 870
Size: 149 721
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 870
Size: 469 401
BsaBI GATnn'nnATC
Cuts at: 0 430 870
Size: 430 440
BsaWI w'CCGG_w
Cuts at: 0 386 477 818 870
Size: 386 91 341 52
Fragments arranged by size:
386 341 91 52
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 168 218 262 265 286 646 870
Size: 88 80 50 44 3 21 360 224
Fragments arranged by size:
360 224 88 80 50 44 21 3
BseYI C'CCAG_C
Cuts at: 0 18 283 567 746 870
Size: 18 265 284 179 124
Fragments arranged by size:
284 265 179 124 18
BsiEI CG_ry'CG
Cuts at: 0 50 870
Size: 50 820
BsiHKAI G_wGCw'C
Cuts at: 0 104 422 870
Size: 104 318 448
Fragments arranged by size:
448 318 104
Bsp1286I G_dGCh'C
Cuts at: 0 104 422 517 696 745 870
Size: 104 318 95 179 49 125
Fragments arranged by size:
318 179 125 104 95 49
BspEI T'CCGG_A
Cuts at: 0 477 870
Size: 477 393
BspHI T'CATG_A
Cuts at: 0 795 870
Size: 795 75
BsrBI CCG'CTC
Cuts at: 0 539 870
Size: 539 331
BsrFI r'CCGG_y
Cuts at: 0 500 858 870
Size: 500 358 12
Fragments arranged by size:
500 358 12
BsrGI T'GTAC_A
Cuts at: 0 578 870
Size: 578 292
BssSI C'ACGA_G
Cuts at: 0 421 870
Size: 421 449
BstXI CCAn_nnnn'nTGG
Cuts at: 0 306 870
Size: 306 564
BstYI r'GATC_y
Cuts at: 0 557 870
Size: 557 313
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 417 870
Size: 417 453
DrdI GACnn_nn'nnGTC
Cuts at: 0 367 870
Size: 367 503
EaeI y'GGCC_r
Cuts at: 0 502 662 842 870
Size: 502 160 180 28
Fragments arranged by size:
502 180 160 28
EarI CTCTTCn'nnn_
Cuts at: 0 817 870
Size: 817 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 55 870
Size: 55 815
EcoICRI GAG'CTC
Cuts at: 0 102 420 870
Size: 102 318 450
Fragments arranged by size:
450 318 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 88 244 730 870
Size: 46 42 156 486 140
Fragments arranged by size:
486 156 140 46 42
EcoO109I rG'GnC_Cy
Cuts at: 0 444 573 610 741 742 870
Size: 444 129 37 131 1 128
Fragments arranged by size:
444 131 129 128 37 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 828 870
Size: 828 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 777 809 870
Size: 777 32 61
Fragments arranged by size:
777 61 32
HincII GTy'rAC
Cuts at: 0 372 870
Size: 372 498
MscI TGG'CCA
Cuts at: 0 844 870
Size: 844 26
MslI CAynn'nnrTG
Cuts at: 0 298 870
Size: 298 572
MspA1I CmG'CkG
Cuts at: 0 471 753 780 870
Size: 471 282 27 90
Fragments arranged by size:
471 282 90 27
PasI CC'CwG_GG
Cuts at: 0 696 870
Size: 696 174
PflMI CCAn_nnn'nTGG
Cuts at: 0 567 870
Size: 567 303
PfoI T'CCnGG_A
Cuts at: 0 560 870
Size: 560 310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 523 555 870
Size: 523 32 315
Fragments arranged by size:
523 315 32
PpuMI rG'GwC_Cy
Cuts at: 0 573 870
Size: 573 297
PspOMI G'GGCC_C
Cuts at: 0 741 870
Size: 741 129
PstI C_TGCA'G
Cuts at: 0 89 785 870
Size: 89 696 85
Fragments arranged by size:
696 89 85
PvuI CG_AT'CG
Cuts at: 0 50 870
Size: 50 820
PvuII CAG'CTG
Cuts at: 0 471 753 870
Size: 471 282 117
Fragments arranged by size:
471 282 117
SacI G_AGCT'C
Cuts at: 0 104 422 870
Size: 104 318 448
Fragments arranged by size:
448 318 104
SfcI C'TryA_G
Cuts at: 0 85 781 870
Size: 85 696 89
Fragments arranged by size:
696 89 85
SmaI CCC'GGG
Cuts at: 0 594 870
Size: 594 276
SmlI C'TyrA_G
Cuts at: 0 448 870
Size: 448 422
StuI AGG'CCT
Cuts at: 0 91 870
Size: 91 779
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 259 870
Size: 259 611
TatI w'GTAC_w
Cuts at: 0 578 870
Size: 578 292
XbaI T'CTAG_A
Cuts at: 0 762 870
Size: 762 108
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 151 870
Size: 151 719
XmaI C'CCGG_G
Cuts at: 0 592 870
Size: 592 278
XmnI GAAnn'nnTTC
Cuts at: 0 193 870
Size: 193 677
Enzymes that do cut:
AcuI AlwNI ApaI AseI AvaI BamHI BanI BanII
BbvCI BcgI BclI BlpI Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaBI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BsrBI BsrFI BsrGI BssSI BstXI BstYI
BtgZI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
FalI Hin4I HincII MscI MslI MspA1I PasI PflMI
PfoI PpiI PpuMI PspOMI PstI PvuI PvuII SacI
SfcI SmaI SmlI StuI TaqII TatI XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AsiSI AvrII BaeI BbeI BbsI BciVI BfrBI BglI
BglII BmrI BmtI BplI BsaI BsaAI BsaHI BsaXI
BsgI BsiWI BsmI BsmBI BspMI BsrDI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtsI ClaI CspCI
DraI DraIII EagI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PshAI
PsiI PspXI PsrI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StyI SwaI TstI Tth111I
XhoI ZraI