(Linear) (Six-base) MAPSORT of: KIBB6343.seq  Check: 424  from: 1  to: 792



KIBB6343.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412     539     792 
   Size:        412     127     253

  Fragments arranged by size: 

                 412     253     127
 
ApoI r'AATT_y

Cuts at:      0     213     398     412     607     792 
   Size:        213     185      14     195     185

  Fragments arranged by size: 

                 213     195     185     185      14
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     755     788     792 
   Size:        755      33       4

  Fragments arranged by size: 

                 755      33       4
 
BanI G'GyrC_C

Cuts at:      0     160     792 
   Size:        160     632
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     791     792 
   Size:        791       1
 
BglII A'GATC_T

Cuts at:      0     190     792 
   Size:        190     602
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     356     792 
   Size:        356     436
 
BsaBI GATnn'nnATC

Cuts at:      0     717     792 
   Size:        717      75
 
BsaWI w'CCGG_w

Cuts at:      0     477     792 
   Size:        477     315
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     311     792 
   Size:        311     481
 
BsiHKAI G_wGCw'C

Cuts at:      0     237     792 
   Size:        237     555
 
Bsp1286I G_dGCh'C

Cuts at:      0     237     792 
   Size:        237     555
 
BspEI T'CCGG_A

Cuts at:      0     477     792 
   Size:        477     315
 
BspHI T'CATG_A

Cuts at:      0      53     792 
   Size:         53     739
 
BsrDI GCAATG_nn'

Cuts at:      0     365     477     792 
   Size:        365     112     315

  Fragments arranged by size: 

                 365     315     112
 
BstYI r'GATC_y

Cuts at:      0     190     336     645     792 
   Size:        190     146     309     147

  Fragments arranged by size: 

                 309     190     147     146
 
Bsu36I CC'TnA_GG

Cuts at:      0     707     792 
   Size:        707      85
 
BtgI C'CryG_G

Cuts at:      0      66     156     792 
   Size:         66      90     636

  Fragments arranged by size: 

                 636      90      66
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     555     590     792 
   Size:        555      35     202

  Fragments arranged by size: 

                 555     202      35
 
DraI TTT'AAA

Cuts at:      0     612     792 
   Size:        612     180
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     356     412     539     792 
   Size:        356      56     127     253

  Fragments arranged by size: 

                 356     253     127      56
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     696     792 
   Size:        696      96
 
EcoRV GAT'ATC

Cuts at:      0     719     792 
   Size:        719      73
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     792 
   Size:        110     682
 
MslI CAynn'nnrTG

Cuts at:      0      71     792 
   Size:         71     721
 
MspA1I CmG'CkG

Cuts at:      0     427     792 
   Size:        427     365
 
NcoI C'CATG_G

Cuts at:      0      66     792 
   Size:         66     726
 
PasI CC'CwG_GG

Cuts at:      0     452     792 
   Size:        452     340
 
PfoI T'CCnGG_A

Cuts at:      0     372     792 
   Size:        372     420
 
PvuII CAG'CTG

Cuts at:      0     427     792 
   Size:        427     365
 
ScaI AGT'ACT

Cuts at:      0     439     792 
   Size:        439     353
 
SfcI C'TryA_G

Cuts at:      0     734     755     792 
   Size:        734      21      37

  Fragments arranged by size: 

                 734      37      21
 
SspI AAT'ATT

Cuts at:      0     729     792 
   Size:        729      63
 
StyI C'CwwG_G

Cuts at:      0      66     792 
   Size:         66     726
 
TatI w'GTAC_w

Cuts at:      0     437     792 
   Size:        437     355
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     321     353     792 
   Size:        321      32     439

  Fragments arranged by size: 

                 439     321      32
 
XbaI T'CTAG_A

Cuts at:      0     229     792 
   Size:        229     563
 

 Enzymes that do cut:

     AcuI     ApoI     BaeI     BanI     BbsI    BglII     BpmI    BsaBI
    BsaWI    BseRI  BsiHKAI Bsp1286I    BspEI    BspHI    BsrDI    BstYI
   Bsu36I     BtgI    CspCI     DraI  Eco57MI    EcoNI    EcoRV     MmeI
     MslI   MspA1I     NcoI     PasI     PfoI    PvuII     ScaI     SfcI
     SspI     StyI     TatI     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII    BamHI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I    BtgZI     BtsI     ClaI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI EcoO109I    EcoRI     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
    TaqII  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI