Restriction map for
pF1KB6344
(Linear) (Six-base) MAPSORT of: KIBB6344.seq Check: 180 from: 1 to: 873
KIBB6344.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 0 620 873
Size: 620 253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 873
Size: 154 719
AfeI AGC'GCT
Cuts at: 0 376 873
Size: 376 497
AlwNI CAG_nnn'CTG
Cuts at: 0 164 837 873
Size: 164 673 36
Fragments arranged by size:
673 164 36
ApaLI G'TGCA_C
Cuts at: 0 249 873
Size: 249 624
ApoI r'AATT_y
Cuts at: 0 547 873
Size: 547 326
AvaI C'yCGr_G
Cuts at: 0 461 873
Size: 461 412
BanI G'GyrC_C
Cuts at: 0 141 541 873
Size: 141 400 332
Fragments arranged by size:
400 332 141
BanII G_rGCy'C
Cuts at: 0 8 873
Size: 8 865
BbeI G_GCGC'C
Cuts at: 0 145 873
Size: 145 728
BbsI GAAGACnn'nnnn_
Cuts at: 0 160 873
Size: 160 713
BglI GCCn_nnn'nGGC
Cuts at: 0 25 873
Size: 25 848
Bme1580I G_kGCm'C
Cuts at: 0 253 873
Size: 253 620
BmrI ACTGGGnnnn_n'
Cuts at: 0 859 873
Size: 859 14
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 673 873
Size: 673 200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 589 814 873
Size: 70 519 225 59
Fragments arranged by size:
519 225 70 59
BsaHI Gr'CG_yC
Cuts at: 0 142 873
Size: 142 731
BsaWI w'CCGG_w
Cuts at: 0 305 873
Size: 305 568
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 873
Size: 124 749
BseYI C'CCAG_C
Cuts at: 0 92 430 486 873
Size: 92 338 56 387
Fragments arranged by size:
387 338 92 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 873
Size: 802 71
BsiHKAI G_wGCw'C
Cuts at: 0 253 873
Size: 253 620
BsiWI C'GTAC_G
Cuts at: 0 452 873
Size: 452 421
BsmI GAATG_Cn'
Cuts at: 0 513 873
Size: 513 360
BsmBI CGTCTCn'nnnn_
Cuts at: 0 853 873
Size: 853 20
Bsp1286I G_dGCh'C
Cuts at: 0 8 253 873
Size: 8 245 620
Fragments arranged by size:
620 245 8
BspEI T'CCGG_A
Cuts at: 0 305 873
Size: 305 568
BspHI T'CATG_A
Cuts at: 0 737 873
Size: 737 136
BsrBI CCG'CTC
Cuts at: 0 710 873
Size: 710 163
BsrFI r'CCGG_y
Cuts at: 0 220 873
Size: 220 653
BstAPI GCAn_nnn'nTGC
Cuts at: 0 233 378 800 873
Size: 233 145 422 73
Fragments arranged by size:
422 233 145 73
BstYI r'GATC_y
Cuts at: 0 317 873
Size: 317 556
BtgI C'CryG_G
Cuts at: 0 321 555 695 873
Size: 321 234 140 178
Fragments arranged by size:
321 234 178 140
BtsI GCAGTG_nn'
Cuts at: 0 286 873
Size: 286 587
ClaI AT'CG_AT
Cuts at: 0 416 873
Size: 416 457
DraIII CAC_nnn'GTG
Cuts at: 0 504 873
Size: 504 369
DrdI GACnn_nn'nnGTC
Cuts at: 0 616 873
Size: 616 257
EarI CTCTTCn'nnn_
Cuts at: 0 442 873
Size: 442 431
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 82 604 873
Size: 82 522 269
Fragments arranged by size:
522 269 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 154 589 814 873
Size: 70 84 435 225 59
Fragments arranged by size:
435 225 84 70 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 809 873
Size: 809 64
EcoO109I rG'GnC_Cy
Cuts at: 0 300 873
Size: 300 573
HaeII r_GCGC'y
Cuts at: 0 145 378 873
Size: 145 233 495
Fragments arranged by size:
495 233 145
KasI G'GCGC_C
Cuts at: 0 141 873
Size: 141 732
MslI CAynn'nnrTG
Cuts at: 0 416 873
Size: 416 457
MspA1I CmG'CkG
Cuts at: 0 486 514 873
Size: 486 28 359
Fragments arranged by size:
486 359 28
NaeI GCC'GGC
Cuts at: 0 222 873
Size: 222 651
NarI GG'CG_CC
Cuts at: 0 142 873
Size: 142 731
NcoI C'CATG_G
Cuts at: 0 321 695 873
Size: 321 374 178
Fragments arranged by size:
374 321 178
NgoMIV G'CCGG_C
Cuts at: 0 220 873
Size: 220 653
PflMI CCAn_nnn'nTGG
Cuts at: 0 279 837 873
Size: 279 558 36
Fragments arranged by size:
558 279 36
PpuMI rG'GwC_Cy
Cuts at: 0 300 873
Size: 300 573
PstI C_TGCA'G
Cuts at: 0 293 374 519 873
Size: 293 81 145 354
Fragments arranged by size:
354 293 145 81
PvuII CAG'CTG
Cuts at: 0 486 873
Size: 486 387
ScaI AGT'ACT
Cuts at: 0 574 873
Size: 574 299
SfcI C'TryA_G
Cuts at: 0 289 370 515 873
Size: 289 81 145 358
Fragments arranged by size:
358 289 145 81
SfoI GGC'GCC
Cuts at: 0 143 873
Size: 143 730
SmaI CCC'GGG
Cuts at: 0 463 873
Size: 463 410
StyI C'CwwG_G
Cuts at: 0 321 669 695 873
Size: 321 348 26 178
Fragments arranged by size:
348 321 178 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 511 873
Size: 511 362
TatI w'GTAC_w
Cuts at: 0 572 873
Size: 572 301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 438 723 873
Size: 438 285 150
Fragments arranged by size:
438 285 150
XmaI C'CCGG_G
Cuts at: 0 461 873
Size: 461 412
Enzymes that do cut:
AccI AcuI AfeI AlwNI ApaLI ApoI AvaI BanI
BanII BbeI BbsI BglI Bme1580I BmrI BplI BpmI
BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI BstAPI BstYI
BtgI BtsI ClaI DraIII DrdI EarI EciI Eco57MI
EcoNI EcoO109I HaeII KasI MslI MspA1I NaeI NarI
NcoI NgoMIV PflMI PpuMI PstI PvuII ScaI SfcI
SfoI SmaI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaXI BsiEI BspMI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI CspCI DraI EaeI
EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XhoI XmnI ZraI