Restriction map for
pF1KB6345
(Linear) (Six-base) MAPSORT of: KIBB6345.seq Check: 3179 from: 1 to: 795
KIBB6345.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 177 795
Size: 177 618
ApaI G_GGCC'C
Cuts at: 0 629 795
Size: 629 166
AvaI C'yCGr_G
Cuts at: 0 338 795
Size: 338 457
BanI G'GyrC_C
Cuts at: 0 289 324 795
Size: 289 35 471
Fragments arranged by size:
471 289 35
BanII G_rGCy'C
Cuts at: 0 91 478 629 795
Size: 91 387 151 166
Fragments arranged by size:
387 166 151 91
BbeI G_GCGC'C
Cuts at: 0 293 795
Size: 293 502
BbsI GAAGACnn'nnnn_
Cuts at: 0 66 778 795
Size: 66 712 17
Fragments arranged by size:
712 66 17
BglI GCCn_nnn'nGGC
Cuts at: 0 473 795
Size: 473 322
BglII A'GATC_T
Cuts at: 0 242 795
Size: 242 553
Bme1580I G_kGCm'C
Cuts at: 0 629 795
Size: 629 166
BmrI ACTGGGnnnn_n'
Cuts at: 0 618 753 795
Size: 618 135 42
Fragments arranged by size:
618 135 42
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 33 795
Size: 33 762
Bpu10I CC'TnA_GC
Cuts at: 0 688 795
Size: 688 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 82 795
Size: 16 66 713
Fragments arranged by size:
713 66 16
BsaAI yAC'GTr
Cuts at: 0 270 725 795
Size: 270 455 70
Fragments arranged by size:
455 270 70
BsaHI Gr'CG_yC
Cuts at: 0 290 795
Size: 290 505
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 763 795
Size: 763 32
BseYI C'CCAG_C
Cuts at: 0 278 480 555 795
Size: 278 202 75 240
Fragments arranged by size:
278 240 202 75
BsiHKAI G_wGCw'C
Cuts at: 0 19 795
Size: 19 776
Bsp1286I G_dGCh'C
Cuts at: 0 19 91 478 629 795
Size: 19 72 387 151 166
Fragments arranged by size:
387 166 151 72 19
BspHI T'CATG_A
Cuts at: 0 584 795
Size: 584 211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 677 795
Size: 677 118
BsrBI CCG'CTC
Cuts at: 0 191 330 563 604 765 795
Size: 191 139 233 41 161 30
Fragments arranged by size:
233 191 161 139 41 30
BsrDI GCAATG_nn'
Cuts at: 0 293 795
Size: 293 502
BstXI CCAn_nnnn'nTGG
Cuts at: 0 219 399 471 795
Size: 219 180 72 324
Fragments arranged by size:
324 219 180 72
BstYI r'GATC_y
Cuts at: 0 242 795
Size: 242 553
BtgI C'CryG_G
Cuts at: 0 20 392 776 795
Size: 20 372 384 19
Fragments arranged by size:
384 372 20 19
EaeI y'GGCC_r
Cuts at: 0 56 103 152 195 350 707 795
Size: 56 47 49 43 155 357 88
Fragments arranged by size:
357 155 88 56 49 47 43
EarI CTCTTCn'nnn_
Cuts at: 0 679 755 795
Size: 679 76 40
Fragments arranged by size:
679 76 40
EcoO109I rG'GnC_Cy
Cuts at: 0 174 181 625 795
Size: 174 7 444 170
Fragments arranged by size:
444 174 170 7
HaeII r_GCGC'y
Cuts at: 0 115 293 795
Size: 115 178 502
Fragments arranged by size:
502 178 115
HincII GTy'rAC
Cuts at: 0 357 795
Size: 357 438
KasI G'GCGC_C
Cuts at: 0 289 795
Size: 289 506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 795
Size: 504 291
MscI TGG'CCA
Cuts at: 0 58 154 709 795
Size: 58 96 555 86
Fragments arranged by size:
555 96 86 58
MslI CAynn'nnrTG
Cuts at: 0 397 795
Size: 397 398
MspA1I CmG'CkG
Cuts at: 0 282 423 756 795
Size: 282 141 333 39
Fragments arranged by size:
333 282 141 39
NarI GG'CG_CC
Cuts at: 0 290 795
Size: 290 505
NcoI C'CATG_G
Cuts at: 0 392 776 795
Size: 392 384 19
Fragments arranged by size:
392 384 19
PasI CC'CwG_GG
Cuts at: 0 177 510 795
Size: 177 333 285
Fragments arranged by size:
333 285 177
PflMI CCAn_nnn'nTGG
Cuts at: 0 177 285 393 795
Size: 177 108 108 402
Fragments arranged by size:
402 177 108 108
PpuMI rG'GwC_Cy
Cuts at: 0 181 795
Size: 181 614
PspOMI G'GGCC_C
Cuts at: 0 625 795
Size: 625 170
PstI C_TGCA'G
Cuts at: 0 131 795
Size: 131 664
PvuII CAG'CTG
Cuts at: 0 282 423 795
Size: 282 141 372
Fragments arranged by size:
372 282 141
SanDI GG'GwC_CC
Cuts at: 0 181 795
Size: 181 614
SapI GCTCTTCn'nnn_
Cuts at: 0 679 755 795
Size: 679 76 40
Fragments arranged by size:
679 76 40
SfcI C'TryA_G
Cuts at: 0 127 795
Size: 127 668
SfoI GGC'GCC
Cuts at: 0 291 795
Size: 291 504
SmaI CCC'GGG
Cuts at: 0 340 795
Size: 340 455
SmlI C'TyrA_G
Cuts at: 0 31 97 795
Size: 31 66 698
Fragments arranged by size:
698 66 31
StyI C'CwwG_G
Cuts at: 0 392 776 795
Size: 392 384 19
Fragments arranged by size:
392 384 19
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 162 795
Size: 162 633
XmaI C'CCGG_G
Cuts at: 0 338 795
Size: 338 457
XmnI GAAnn'nnTTC
Cuts at: 0 592 795
Size: 592 203
Enzymes that do cut:
AlwNI ApaI AvaI BanI BanII BbeI BbsI BglI
BglII Bme1580I BmrI BplI Bpu10I BpuEI BsaAI BsaHI
BseRI BseYI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BstXI BstYI BtgI EaeI EarI EcoO109I HaeII HincII
KasI MmeI MscI MslI MspA1I NarI NcoI PasI
PflMI PpuMI PspOMI PstI PvuII SanDI SapI SfcI
SfoI SmaI SmlI StyI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BlpI BmgBI BmtI BpmI BsaI
BsaBI BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HindIII HpaI KpnI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
TstI Tth111I XbaI XhoI ZraI