Restriction map for
pF1KB6348
(Linear) (Six-base) MAPSORT of: KIBB6348.seq Check: 3978 from: 1 to: 879
KIBB6348.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 0 22 879
Size: 22 857
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 800 879
Size: 68 732 79
Fragments arranged by size:
732 79 68
AlwNI CAG_nnn'CTG
Cuts at: 0 592 879
Size: 592 287
ApaLI G'TGCA_C
Cuts at: 0 657 879
Size: 657 222
ApoI r'AATT_y
Cuts at: 0 244 879
Size: 244 635
AseI AT'TA_AT
Cuts at: 0 310 532 879
Size: 310 222 347
Fragments arranged by size:
347 310 222
BbsI GAAGACnn'nnnn_
Cuts at: 0 67 698 879
Size: 67 631 181
Fragments arranged by size:
631 181 67
Bme1580I G_kGCm'C
Cuts at: 0 661 879
Size: 661 218
BmgBI CAC'GTC
Cuts at: 0 78 879
Size: 78 801
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 879
Size: 33 846
BsaBI GATnn'nnATC
Cuts at: 0 392 879
Size: 392 487
BsaHI Gr'CG_yC
Cuts at: 0 278 879
Size: 278 601
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 75 105 879
Size: 75 30 774
Fragments arranged by size:
774 75 30
BseYI C'CCAG_C
Cuts at: 0 171 879
Size: 171 708
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 307 879
Size: 86 221 572
Fragments arranged by size:
572 221 86
BsiHKAI G_wGCw'C
Cuts at: 0 661 879
Size: 661 218
Bsp1286I G_dGCh'C
Cuts at: 0 661 879
Size: 661 218
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 516 879
Size: 516 363
BsrGI T'GTAC_A
Cuts at: 0 732 879
Size: 732 147
Bsu36I CC'TnA_GG
Cuts at: 0 462 879
Size: 462 417
BtgI C'CryG_G
Cuts at: 0 718 879
Size: 718 161
BtsI GCAGTG_nn'
Cuts at: 0 659 879
Size: 659 220
DraI TTT'AAA
Cuts at: 0 627 879
Size: 627 252
EarI CTCTTCn'nnn_
Cuts at: 0 50 239 475 854 879
Size: 50 189 236 379 25
Fragments arranged by size:
379 236 189 50 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 519 879
Size: 519 360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 68 800 879
Size: 33 35 732 79
Fragments arranged by size:
732 79 35 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 445 477 747 779 879
Size: 445 32 270 32 100
Fragments arranged by size:
445 270 100 32 32
HincII GTy'rAC
Cuts at: 0 185 642 879
Size: 185 457 237
Fragments arranged by size:
457 237 185
MslI CAynn'nnrTG
Cuts at: 0 312 879
Size: 312 567
MspA1I CmG'CkG
Cuts at: 0 117 879
Size: 117 762
NcoI C'CATG_G
Cuts at: 0 718 879
Size: 718 161
NspI r_CATG'y
Cuts at: 0 21 202 879
Size: 21 181 677
Fragments arranged by size:
677 181 21
PstI C_TGCA'G
Cuts at: 0 597 879
Size: 597 282
PvuII CAG'CTG
Cuts at: 0 117 879
Size: 117 762
ScaI AGT'ACT
Cuts at: 0 599 879
Size: 599 280
SfcI C'TryA_G
Cuts at: 0 593 879
Size: 593 286
SphI G_CATG'C
Cuts at: 0 202 879
Size: 202 677
SspI AAT'ATT
Cuts at: 0 329 879
Size: 329 550
StyI C'CwwG_G
Cuts at: 0 251 718 879
Size: 251 467 161
Fragments arranged by size:
467 251 161
TatI w'GTAC_w
Cuts at: 0 597 732 840 879
Size: 597 135 108 39
Fragments arranged by size:
597 135 108 39
XmnI GAAnn'nnTTC
Cuts at: 0 7 879
Size: 7 872
Enzymes that do cut:
AccI AcuI AlwNI ApaLI ApoI AseI BbsI Bme1580I
BmgBI BpmI BsaBI BsaHI BsaXI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrGI Bsu36I BtgI BtsI DraI EarI
EciI Eco57MI Hin4I HincII MslI MspA1I NcoI NspI
PstI PvuII ScaI SfcI SphI SspI StyI TatI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaWI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgZI ClaI CspCI DraIII DrdI EaeI EagI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI StuI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI ZraI