Restriction map for
pF1KB6349
(Linear) (Six-base) MAPSORT of: KIBB6349.seq Check: 4223 from: 1 to: 798
KIBB6349.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 610 798
Size: 610 188
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 732 760 798
Size: 31 701 28 38
Fragments arranged by size:
701 38 31 28
AfeI AGC'GCT
Cuts at: 0 41 798
Size: 41 757
AlwNI CAG_nnn'CTG
Cuts at: 0 411 558 798
Size: 411 147 240
Fragments arranged by size:
411 240 147
BanII G_rGCy'C
Cuts at: 0 70 798
Size: 70 728
BlpI GC'TnA_GC
Cuts at: 0 64 798
Size: 64 734
BmgBI CAC'GTC
Cuts at: 0 216 798
Size: 216 582
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 310 798
Size: 41 269 488
Fragments arranged by size:
488 269 41
Bpu10I CC'TnA_GC
Cuts at: 0 793 798
Size: 793 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 798
Size: 205 593
BseYI C'CCAG_C
Cuts at: 0 243 404 505 708 798
Size: 243 161 101 203 90
Fragments arranged by size:
243 203 161 101 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 653 798
Size: 379 274 145
Fragments arranged by size:
379 274 145
BsiHKAI G_wGCw'C
Cuts at: 0 70 617 657 798
Size: 70 547 40 141
Fragments arranged by size:
547 141 70 40
Bsp1286I G_dGCh'C
Cuts at: 0 70 617 657 798
Size: 70 547 40 141
Fragments arranged by size:
547 141 70 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 610 798
Size: 610 188
BsrBI CCG'CTC
Cuts at: 0 153 798
Size: 153 645
Bsu36I CC'TnA_GG
Cuts at: 0 377 798
Size: 377 421
BtgI C'CryG_G
Cuts at: 0 305 798
Size: 305 493
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 62 97 798
Size: 62 35 701
Fragments arranged by size:
701 62 35
EaeI y'GGCC_r
Cuts at: 0 489 798
Size: 489 309
EarI CTCTTCn'nnn_
Cuts at: 0 491 724 798
Size: 491 233 74
Fragments arranged by size:
491 233 74
EcoICRI GAG'CTC
Cuts at: 0 68 798
Size: 68 730
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 41 310 732 760 798
Size: 31 10 269 422 28 38
Fragments arranged by size:
422 269 38 31 28 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 292 798
Size: 292 506
HaeII r_GCGC'y
Cuts at: 0 43 798
Size: 43 755
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 114 146 375 407 618 650 798
Size: 114 32 229 32 211 32 148
Fragments arranged by size:
229 211 148 114 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 798
Size: 396 402
MspA1I CmG'CkG
Cuts at: 0 307 363 798
Size: 307 56 435
Fragments arranged by size:
435 307 56
PflMI CCAn_nnn'nTGG
Cuts at: 0 558 798
Size: 558 240
PfoI T'CCnGG_A
Cuts at: 0 164 287 798
Size: 164 123 511
Fragments arranged by size:
511 164 123
PstI C_TGCA'G
Cuts at: 0 325 416 798
Size: 325 91 382
Fragments arranged by size:
382 325 91
SacI G_AGCT'C
Cuts at: 0 70 798
Size: 70 728
SacII CC_GC'GG
Cuts at: 0 308 798
Size: 308 490
SapI GCTCTTCn'nnn_
Cuts at: 0 724 798
Size: 724 74
ScaI AGT'ACT
Cuts at: 0 265 798
Size: 265 533
SfcI C'TryA_G
Cuts at: 0 321 412 798
Size: 321 91 386
Fragments arranged by size:
386 321 91
StuI AGG'CCT
Cuts at: 0 524 798
Size: 524 274
StyI C'CwwG_G
Cuts at: 0 723 798
Size: 723 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 632 798
Size: 632 166
TatI w'GTAC_w
Cuts at: 0 263 798
Size: 263 535
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 107 798
Size: 107 691
XmnI GAAnn'nnTTC
Cuts at: 0 575 798
Size: 575 223
Enzymes that do cut:
AarI AcuI AfeI AlwNI BanII BlpI BmgBI BpmI
Bpu10I BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI
Bsu36I BtgI CspCI EaeI EarI EcoICRI Eco57MI EcoNI
HaeII Hin4I MmeI MspA1I PflMI PfoI PstI SacI
SacII SapI ScaI SfcI StuI StyI TaqII TatI
XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII Bme1580I
BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgZI BtsI ClaI DraI DraIII
DrdI EagI EciI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI
XmaI ZraI