Restriction map for
pF1KB6354
(Linear) (Six-base) MAPSORT of: KIBB6354.seq Check: 4654 from: 1 to: 885
KIBB6354.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 348 885
Size: 348 537
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 885
Size: 715 170
AleI CACnn'nnGTG
Cuts at: 0 814 885
Size: 814 71
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 664 696 885
Size: 664 32 189
Fragments arranged by size:
664 189 32
ApaI G_GGCC'C
Cuts at: 0 13 885
Size: 13 872
ApaLI G'TGCA_C
Cuts at: 0 732 885
Size: 732 153
BamHI G'GATC_C
Cuts at: 0 236 885
Size: 236 649
BanII G_rGCy'C
Cuts at: 0 13 61 74 885
Size: 13 48 13 811
Fragments arranged by size:
811 48 13 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 444 478 885
Size: 444 34 407
Fragments arranged by size:
444 407 34
BclI T'GATC_A
Cuts at: 0 806 885
Size: 806 79
Bme1580I G_kGCm'C
Cuts at: 0 13 736 885
Size: 13 723 149
Fragments arranged by size:
723 149 13
BmtI G_CTAG'C
Cuts at: 0 470 885
Size: 470 415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 661 885
Size: 238 423 224
Fragments arranged by size:
423 238 224
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 885
Size: 439 446
BsaI GGTCTCn'nnnn_
Cuts at: 0 193 319 637 885
Size: 193 126 318 248
Fragments arranged by size:
318 248 193 126
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 757 885
Size: 757 128
BseYI C'CCAG_C
Cuts at: 0 189 885
Size: 189 696
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 885
Size: 376 509
BsiEI CG_ry'CG
Cuts at: 0 429 885
Size: 429 456
BsiHKAI G_wGCw'C
Cuts at: 0 289 736 885
Size: 289 447 149
Fragments arranged by size:
447 289 149
Bsp1286I G_dGCh'C
Cuts at: 0 13 61 74 289 736 885
Size: 13 48 13 215 447 149
Fragments arranged by size:
447 215 149 48 13 13
BspHI T'CATG_A
Cuts at: 0 248 885
Size: 248 637
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 146 194 348 885
Size: 146 48 154 537
Fragments arranged by size:
537 154 146 48
BsrFI r'CCGG_y
Cuts at: 0 226 885
Size: 226 659
BssHII G'CGCG_C
Cuts at: 0 64 885
Size: 64 821
BssSI C'ACGA_G
Cuts at: 0 196 885
Size: 196 689
BstAPI GCAn_nnn'nTGC
Cuts at: 0 116 885
Size: 116 769
BstXI CCAn_nnnn'nTGG
Cuts at: 0 189 885
Size: 189 696
BstYI r'GATC_y
Cuts at: 0 236 885
Size: 236 649
BtgI C'CryG_G
Cuts at: 0 150 885
Size: 150 735
BtsI GCAGTG_nn'
Cuts at: 0 45 262 736 885
Size: 45 217 474 149
Fragments arranged by size:
474 217 149 45
EaeI y'GGCC_r
Cuts at: 0 84 131 885
Size: 84 47 754
Fragments arranged by size:
754 84 47
EarI CTCTTCn'nnn_
Cuts at: 0 37 79 298 885
Size: 37 42 219 587
Fragments arranged by size:
587 219 42 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 378 885
Size: 378 507
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 661 715 885
Size: 238 423 54 170
Fragments arranged by size:
423 238 170 54
EcoO109I rG'GnC_Cy
Cuts at: 0 9 10 690 885
Size: 9 1 680 195
Fragments arranged by size:
680 195 9 1
HaeII r_GCGC'y
Cuts at: 0 233 885
Size: 233 652
MfeI C'AATT_G
Cuts at: 0 114 885
Size: 114 771
MscI TGG'CCA
Cuts at: 0 86 885
Size: 86 799
MslI CAynn'nnrTG
Cuts at: 0 814 885
Size: 814 71
MspA1I CmG'CkG
Cuts at: 0 144 243 371 885
Size: 144 99 128 514
Fragments arranged by size:
514 144 128 99
NaeI GCC'GGC
Cuts at: 0 228 885
Size: 228 657
NgoMIV G'CCGG_C
Cuts at: 0 226 885
Size: 226 659
NheI G'CTAG_C
Cuts at: 0 466 885
Size: 466 419
NspI r_CATG'y
Cuts at: 0 174 417 885
Size: 174 243 468
Fragments arranged by size:
468 243 174
PfoI T'CCnGG_A
Cuts at: 0 638 885
Size: 638 247
PpuMI rG'GwC_Cy
Cuts at: 0 690 885
Size: 690 195
PspOMI G'GGCC_C
Cuts at: 0 9 885
Size: 9 876
PstI C_TGCA'G
Cuts at: 0 8 143 881 885
Size: 8 135 738 4
Fragments arranged by size:
738 135 8 4
PvuII CAG'CTG
Cuts at: 0 144 885
Size: 144 741
SapI GCTCTTCn'nnn_
Cuts at: 0 37 79 298 885
Size: 37 42 219 587
Fragments arranged by size:
587 219 42 37
SfcI C'TryA_G
Cuts at: 0 4 139 877 885
Size: 4 135 738 8
Fragments arranged by size:
738 135 8 4
SmlI C'TyrA_G
Cuts at: 0 418 885
Size: 418 467
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 636 885
Size: 636 249
Enzymes that do cut:
AarI AcuI AleI AloI ApaI ApaLI BamHI BanII
BcgI BclI Bme1580I BmtI BpmI BpuEI BsaI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrFI
BssHII BssSI BstAPI BstXI BstYI BtgI BtsI EaeI
EarI EciI Eco57MI EcoO109I HaeII MfeI MscI MslI
MspA1I NaeI NgoMIV NheI NspI PfoI PpuMI PspOMI
PstI PvuII SapI SfcI SmlI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AlwNI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BanI BbeI BbsI BbvCI BciVI BfrBI
BglI BglII BlpI BmgBI BmrI BplI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsaXI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BsrGI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI NarI NcoI
NdeI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI