(Linear) (Six-base) MAPSORT of: KIBB6355.seq Check: 9619 from: 1 to: 798 KIBB6355.orf With 182 enzymes: SgfI * June 26, 2008 10:46 .. ApoI r'AATT_y Cuts at: 0 119 165 242 315 417 475 606 798 Size: 119 46 77 73 102 58 131 192 Fragments arranged by size: 192 131 119 102 77 73 58 46 BanI G'GyrC_C Cuts at: 0 566 798 Size: 566 232 BanII G_rGCy'C Cuts at: 0 74 798 Size: 74 724 BbeI G_GCGC'C Cuts at: 0 570 798 Size: 570 228 BbsI GAAGACnn'nnnn_ Cuts at: 0 542 719 798 Size: 542 177 79 Fragments arranged by size: 542 177 79 BclI T'GATC_A Cuts at: 0 660 798 Size: 660 138 BglII A'GATC_T Cuts at: 0 397 798 Size: 397 401 BmrI ACTGGGnnnn_n' Cuts at: 0 555 798 Size: 555 243 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 395 753 798 Size: 395 358 45 Fragments arranged by size: 395 358 45 BsaHI Gr'CG_yC Cuts at: 0 567 798 Size: 567 231 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 82 798 Size: 82 716 BseYI C'CCAG_C Cuts at: 0 97 798 Size: 97 701 BsiHKAI G_wGCw'C Cuts at: 0 74 798 Size: 74 724 Bsp1286I G_dGCh'C Cuts at: 0 74 798 Size: 74 724 BspHI T'CATG_A Cuts at: 0 723 798 Size: 723 75 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 335 798 Size: 335 463 BsrDI GCAATG_nn' Cuts at: 0 203 798 Size: 203 595 BstXI CCAn_nnnn'nTGG Cuts at: 0 97 798 Size: 97 701 BstYI r'GATC_y Cuts at: 0 397 798 Size: 397 401 BtsI GCAGTG_nn' Cuts at: 0 440 798 Size: 440 358 EarI CTCTTCn'nnn_ Cuts at: 0 411 750 798 Size: 411 339 48 Fragments arranged by size: 411 339 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 31 798 Size: 31 767 EcoICRI GAG'CTC Cuts at: 0 72 798 Size: 72 726 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 395 753 798 Size: 395 358 45 Fragments arranged by size: 395 358 45 EcoRI G'AATT_C Cuts at: 0 606 798 Size: 606 192 HaeII r_GCGC'y Cuts at: 0 570 786 798 Size: 570 216 12 Fragments arranged by size: 570 216 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 652 684 798 Size: 652 32 114 Fragments arranged by size: 652 114 32 KasI G'GCGC_C Cuts at: 0 566 798 Size: 566 232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 291 798 Size: 291 507 MslI CAynn'nnrTG Cuts at: 0 743 798 Size: 743 55 NarI GG'CG_CC Cuts at: 0 567 798 Size: 567 231 NdeI CA'TA_TG Cuts at: 0 465 798 Size: 465 333 PshAI GACnn'nnGTC Cuts at: 0 459 798 Size: 459 339 SacI G_AGCT'C Cuts at: 0 74 798 Size: 74 724 SfoI GGC'GCC Cuts at: 0 568 798 Size: 568 230 StuI AGG'CCT Cuts at: 0 234 798 Size: 234 564 StyI C'CwwG_G Cuts at: 0 570 798 Size: 570 228 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 79 798 Size: 79 719 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 307 798 Size: 307 491 XmnI GAAnn'nnTTC Cuts at: 0 419 606 798 Size: 419 187 192 Fragments arranged by size: 419 192 187 Enzymes that do cut: ApoI BanI BanII BbeI BbsI BclI BglII BmrI BpmI BsaHI BseRI BseYI BsiHKAI Bsp1286I BspHI BspMI BsrDI BstXI BstYI BtsI EarI EciI EcoICRI Eco57MI EcoRI HaeII Hin4I KasI MmeI MslI NarI NdeI PshAI SacI SfoI StuI StyI TaqII XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI MspA1I NaeI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI XmaI ZraI