(Linear) (Six-base) MAPSORT of: KIBB6355.seq Check: 9619 from: 1 to: 798
KIBB6355.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
ApoI r'AATT_y
Cuts at: 0 119 165 242 315 417 475 606 798
Size: 119 46 77 73 102 58 131 192
Fragments arranged by size:
192 131 119 102 77 73 58 46
BanI G'GyrC_C
Cuts at: 0 566 798
Size: 566 232
BanII G_rGCy'C
Cuts at: 0 74 798
Size: 74 724
BbeI G_GCGC'C
Cuts at: 0 570 798
Size: 570 228
BbsI GAAGACnn'nnnn_
Cuts at: 0 542 719 798
Size: 542 177 79
Fragments arranged by size:
542 177 79
BclI T'GATC_A
Cuts at: 0 660 798
Size: 660 138
BglII A'GATC_T
Cuts at: 0 397 798
Size: 397 401
BmrI ACTGGGnnnn_n'
Cuts at: 0 555 798
Size: 555 243
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 753 798
Size: 395 358 45
Fragments arranged by size:
395 358 45
BsaHI Gr'CG_yC
Cuts at: 0 567 798
Size: 567 231
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 82 798
Size: 82 716
BseYI C'CCAG_C
Cuts at: 0 97 798
Size: 97 701
BsiHKAI G_wGCw'C
Cuts at: 0 74 798
Size: 74 724
Bsp1286I G_dGCh'C
Cuts at: 0 74 798
Size: 74 724
BspHI T'CATG_A
Cuts at: 0 723 798
Size: 723 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 335 798
Size: 335 463
BsrDI GCAATG_nn'
Cuts at: 0 203 798
Size: 203 595
BstXI CCAn_nnnn'nTGG
Cuts at: 0 97 798
Size: 97 701
BstYI r'GATC_y
Cuts at: 0 397 798
Size: 397 401
BtsI GCAGTG_nn'
Cuts at: 0 440 798
Size: 440 358
EarI CTCTTCn'nnn_
Cuts at: 0 411 750 798
Size: 411 339 48
Fragments arranged by size:
411 339 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 31 798
Size: 31 767
EcoICRI GAG'CTC
Cuts at: 0 72 798
Size: 72 726
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 753 798
Size: 395 358 45
Fragments arranged by size:
395 358 45
EcoRI G'AATT_C
Cuts at: 0 606 798
Size: 606 192
HaeII r_GCGC'y
Cuts at: 0 570 786 798
Size: 570 216 12
Fragments arranged by size:
570 216 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 652 684 798
Size: 652 32 114
Fragments arranged by size:
652 114 32
KasI G'GCGC_C
Cuts at: 0 566 798
Size: 566 232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 798
Size: 291 507
MslI CAynn'nnrTG
Cuts at: 0 743 798
Size: 743 55
NarI GG'CG_CC
Cuts at: 0 567 798
Size: 567 231
NdeI CA'TA_TG
Cuts at: 0 465 798
Size: 465 333
PshAI GACnn'nnGTC
Cuts at: 0 459 798
Size: 459 339
SacI G_AGCT'C
Cuts at: 0 74 798
Size: 74 724
SfoI GGC'GCC
Cuts at: 0 568 798
Size: 568 230
StuI AGG'CCT
Cuts at: 0 234 798
Size: 234 564
StyI C'CwwG_G
Cuts at: 0 570 798
Size: 570 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 79 798
Size: 79 719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 307 798
Size: 307 491
XmnI GAAnn'nnTTC
Cuts at: 0 419 606 798
Size: 419 187 192
Fragments arranged by size:
419 192 187
Enzymes that do cut:
ApoI BanI BanII BbeI BbsI BclI BglII BmrI
BpmI BsaHI BseRI BseYI BsiHKAI Bsp1286I BspHI BspMI
BsrDI BstXI BstYI BtsI EarI EciI EcoICRI Eco57MI
EcoRI HaeII Hin4I KasI MmeI MslI NarI NdeI
PshAI SacI SfoI StuI StyI TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EaeI EagI EcoNI EcoO109I EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MscI
MspA1I NaeI NcoI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TatI TstI Tth111I
XbaI XhoI XmaI ZraI