Restriction map for
pF1KB6358
(Linear) (Six-base) MAPSORT of: KIBB6358.seq Check: 4368 from: 1 to: 888
KIBB6358.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 702 888
Size: 702 186
AccI GT'mk_AC
Cuts at: 0 830 888
Size: 830 58
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 385 888
Size: 385 503
ApaI G_GGCC'C
Cuts at: 0 855 888
Size: 855 33
AvaI C'yCGr_G
Cuts at: 0 37 151 295 621 854 888
Size: 37 114 144 326 233 34
Fragments arranged by size:
326 233 144 114 37 34
BanI G'GyrC_C
Cuts at: 0 250 291 356 888
Size: 250 41 65 532
Fragments arranged by size:
532 250 65 41
BanII G_rGCy'C
Cuts at: 0 656 855 888
Size: 656 199 33
Fragments arranged by size:
656 199 33
BbeI G_GCGC'C
Cuts at: 0 254 360 888
Size: 254 106 528
Fragments arranged by size:
528 254 106
BbvCI CC'TCA_GC
Cuts at: 0 80 557 888
Size: 80 477 331
Fragments arranged by size:
477 331 80
BglII A'GATC_T
Cuts at: 0 755 888
Size: 755 133
Bme1580I G_kGCm'C
Cuts at: 0 296 725 855 888
Size: 296 429 130 33
Fragments arranged by size:
429 296 130 33
BmgBI CAC'GTC
Cuts at: 0 414 888
Size: 414 474
BmrI ACTGGGnnnn_n'
Cuts at: 0 576 888
Size: 576 312
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 456 476 888
Size: 456 20 412
Fragments arranged by size:
456 412 20
Bpu10I CC'TnA_GC
Cuts at: 0 80 346 557 888
Size: 80 266 211 331
Fragments arranged by size:
331 266 211 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 888
Size: 253 635
BsaBI GATnn'nnATC
Cuts at: 0 754 888
Size: 754 134
BsaHI Gr'CG_yC
Cuts at: 0 251 357 788 888
Size: 251 106 431 100
Fragments arranged by size:
431 251 106 100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 493 523 888
Size: 493 30 365
Fragments arranged by size:
493 365 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 70 82 97 100 124 127 325
Size: 55 15 12 15 3 24 3 198
Cuts at: 325 328 460 501 586 888
Size: 3 132 41 85 302
Fragments arranged by size:
302 198 132 85 55 41 24 15
15 12 3 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 888
Size: 223 665
BsiEI CG_ry'CG
Cuts at: 0 94 125 191 888
Size: 94 31 66 697
Fragments arranged by size:
697 94 66 31
BsiHKAI G_wGCw'C
Cuts at: 0 656 680 812 888
Size: 656 24 132 76
Fragments arranged by size:
656 132 76 24
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2 888
Size: 2 886
Bsp1286I G_dGCh'C
Cuts at: 0 296 656 680 725 812 855 888
Size: 296 360 24 45 87 43 33
Fragments arranged by size:
360 296 87 45 43 33 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 557 702 888
Size: 557 145 186
Fragments arranged by size:
557 186 145
BsrBI CCG'CTC
Cuts at: 0 90 888
Size: 90 798
BsrFI r'CCGG_y
Cuts at: 0 186 594 888
Size: 186 408 294
Fragments arranged by size:
408 294 186
BssSI C'ACGA_G
Cuts at: 0 679 888
Size: 679 209
BstEII G'GTnAC_C
Cuts at: 0 13 858 888
Size: 13 845 30
Fragments arranged by size:
845 30 13
BstYI r'GATC_y
Cuts at: 0 755 888
Size: 755 133
BtsI GCAGTG_nn'
Cuts at: 0 181 725 888
Size: 181 544 163
Fragments arranged by size:
544 181 163
EaeI y'GGCC_r
Cuts at: 0 91 122 176 188 596 888
Size: 91 31 54 12 408 292
Fragments arranged by size:
408 292 91 54 31 12
EagI C'GGCC_G
Cuts at: 0 91 122 188 888
Size: 91 31 66 700
Fragments arranged by size:
700 91 66 31
EarI CTCTTCn'nnn_
Cuts at: 0 383 888
Size: 383 505
EcoICRI GAG'CTC
Cuts at: 0 654 888
Size: 654 234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 456 476 888
Size: 456 20 412
Fragments arranged by size:
456 412 20
EcoO109I rG'GnC_Cy
Cuts at: 0 851 888
Size: 851 37
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 519 888
Size: 519 369
HaeII r_GCGC'y
Cuts at: 0 254 360 888
Size: 254 106 528
Fragments arranged by size:
528 254 106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 604 636 888
Size: 604 32 252
Fragments arranged by size:
604 252 32
KasI G'GCGC_C
Cuts at: 0 250 356 888
Size: 250 106 532
Fragments arranged by size:
532 250 106
MslI CAynn'nnrTG
Cuts at: 0 604 888
Size: 604 284
MspA1I CmG'CkG
Cuts at: 0 159 258 707 888
Size: 159 99 449 181
Fragments arranged by size:
449 181 159 99
NaeI GCC'GGC
Cuts at: 0 188 596 888
Size: 188 408 292
Fragments arranged by size:
408 292 188
NarI GG'CG_CC
Cuts at: 0 251 357 888
Size: 251 106 531
Fragments arranged by size:
531 251 106
NgoMIV G'CCGG_C
Cuts at: 0 186 594 888
Size: 186 408 294
Fragments arranged by size:
408 294 186
NotI GC'GGCC_GC
Cuts at: 0 122 888
Size: 122 766
PspOMI G'GGCC_C
Cuts at: 0 851 888
Size: 851 37
PstI C_TGCA'G
Cuts at: 0 554 888
Size: 554 334
SacI G_AGCT'C
Cuts at: 0 656 888
Size: 656 232
SbfI CC_TGCA'GG
Cuts at: 0 554 888
Size: 554 334
ScaI AGT'ACT
Cuts at: 0 502 888
Size: 502 386
SfcI C'TryA_G
Cuts at: 0 550 888
Size: 550 338
SfoI GGC'GCC
Cuts at: 0 252 358 888
Size: 252 106 530
Fragments arranged by size:
530 252 106
SmaI CCC'GGG
Cuts at: 0 856 888
Size: 856 32
SmlI C'TyrA_G
Cuts at: 0 268 888
Size: 268 620
StuI AGG'CCT
Cuts at: 0 208 888
Size: 208 680
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 493 888
Size: 493 395
TatI w'GTAC_w
Cuts at: 0 500 888
Size: 500 388
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 391 423 888
Size: 391 32 465
Fragments arranged by size:
465 391 32
Tth111I GACn'n_nGTC
Cuts at: 0 826 888
Size: 826 62
XmaI C'CCGG_G
Cuts at: 0 854 888
Size: 854 34
XmnI GAAnn'nnTTC
Cuts at: 0 502 888
Size: 502 386
Enzymes that do cut:
AarI AccI AlfI ApaI AvaI BanI BanII BbeI
BbvCI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaBI BsaHI BsaXI BseRI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrFI BssSI BstEII BstYI BtsI
EaeI EagI EarI EcoICRI Eco57MI EcoO109I FalI HaeII
Hin4I KasI MslI MspA1I NaeI NarI NgoMIV NotI
PspOMI PstI SacI SbfI ScaI SfcI SfoI SmaI
SmlI StuI TaqII TatI TstI Tth111I XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI
BfrBI BglI BlpI BmtI BplI BsaI BsaAI BsaWI
BseYI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII
BstAPI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EciI EcoNI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI NcoI NdeI NheI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacII SalI SanDI SapI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StyI SwaI
XbaI XcmI XhoI ZraI