(Linear) (Six-base) MAPSORT of: KIBB6359.seq  Check: 8167  from: 1  to: 762



KIBB6359.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     154     762 
   Size:         34     120     608

  Fragments arranged by size: 

                 608     120      34
 
AlwNI CAG_nnn'CTG

Cuts at:      0     199     342     426     762 
   Size:        199     143      84     336

  Fragments arranged by size: 

                 336     199     143      84
 
ApoI r'AATT_y

Cuts at:      0      93     762 
   Size:         93     669
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      86     762 
   Size:         86     676
 
BbvCI CC'TCA_GC

Cuts at:      0     670     762 
   Size:        670      92
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     187     762 
   Size:        187     575
 
Bpu10I CC'TnA_GC

Cuts at:      0     670     762 
   Size:        670      92
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      22     762 
   Size:         22     740
 
BsrBI CCG'CTC

Cuts at:      0      60     762 
   Size:         60     702
 
BsrDI GCAATG_nn'

Cuts at:      0     750     762 
   Size:        750      12
 
BstYI r'GATC_y

Cuts at:      0     170     374     762 
   Size:        170     204     388

  Fragments arranged by size: 

                 388     204     170
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     239     762 
   Size:        239     523
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     154     762 
   Size:         34     120     608

  Fragments arranged by size: 

                 608     120      34
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     762 
   Size:        245     517
 
MspA1I CmG'CkG

Cuts at:      0     342     762 
   Size:        342     420
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     342     762 
   Size:        342     420
 
PsiI TTA'TAA

Cuts at:      0     251     762 
   Size:        251     511
 
PvuII CAG'CTG

Cuts at:      0     342     762 
   Size:        342     420
 
SmlI C'TyrA_G

Cuts at:      0      37     762 
   Size:         37     725
 
StyI C'CwwG_G

Cuts at:      0     288     296     762 
   Size:        288       8     466

  Fragments arranged by size: 

                 466     288       8
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      54      86     284     316     762 
   Size:         54      32     198      32     446

  Fragments arranged by size: 

                 446     198      54      32      32
 
XbaI T'CTAG_A

Cuts at:      0     538     762 
   Size:        538     224
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     BbsI    BbvCI     BmrI   Bpu10I    BpuEI
    BsrBI    BsrDI    BstYI     DrdI  Eco57MI     MmeI   MspA1I    PflMI
     PsiI    PvuII     SmlI     StyI     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI     BpmI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI