(Linear) (Six-base) MAPSORT of: KIBB6360.seq  Check: 4449  from: 1  to: 891



KIBB6360.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     442     891 
   Size:        442     449
 
Acc65I G'GTAC_C

Cuts at:      0     129     891 
   Size:        129     762
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     838     891 
   Size:        838      53
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     716     891 
   Size:        716     175
 
AlwNI CAG_nnn'CTG

Cuts at:      0     328     816     891 
   Size:        328     488      75

  Fragments arranged by size: 

                 488     328      75
 
ApoI r'AATT_y

Cuts at:      0     479     666     891 
   Size:        479     187     225

  Fragments arranged by size: 

                 479     225     187
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     121     154     891 
   Size:        121      33     737

  Fragments arranged by size: 

                 737     121      33
 
BanI G'GyrC_C

Cuts at:      0     129     535     891 
   Size:        129     406     356

  Fragments arranged by size: 

                 406     356     129
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      76     891 
   Size:         76     815
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     624     891 
   Size:        624     267
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25     891 
   Size:         25     866
 
BseYI C'CCAG_C

Cuts at:      0     347     635     891 
   Size:        347     288     256

  Fragments arranged by size: 

                 347     288     256
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     470     891 
   Size:        313     157     421

  Fragments arranged by size: 

                 421     313     157
 
BsmI GAATG_Cn'

Cuts at:      0     705     891 
   Size:        705     186
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     442     891 
   Size:        442     449
 
BsrGI T'GTAC_A

Cuts at:      0     469     891 
   Size:        469     422
 
BstYI r'GATC_y

Cuts at:      0     312     891 
   Size:        312     579
 
BtsI GCAGTG_nn'

Cuts at:      0      60     891 
   Size:         60     831
 
DraIII CAC_nnn'GTG

Cuts at:      0     753     891 
   Size:        753     138
 
EarI CTCTTCn'nnn_

Cuts at:      0       2     593     731     891 
   Size:          2     591     138     160

  Fragments arranged by size: 

                 591     160     138       2
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     781     891 
   Size:        781     110
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     838     891 
   Size:        838      53
 
EcoRI G'AATT_C

Cuts at:      0     666     891 
   Size:        666     225
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     624     656     891 
   Size:        624      32     235

  Fragments arranged by size: 

                 624     235      32
 
HincII GTy'rAC

Cuts at:      0     743     891 
   Size:        743     148
 
HindIII A'AGCT_T

Cuts at:      0     229     891 
   Size:        229     662
 
HpaI GTT'AAC

Cuts at:      0     743     891 
   Size:        743     148
 
KpnI G_GTAC'C

Cuts at:      0     133     891 
   Size:        133     758
 
PfoI T'CCnGG_A

Cuts at:      0     722     891 
   Size:        722     169
 
ScaI AGT'ACT

Cuts at:      0     243     891 
   Size:        243     648
 
SfcI C'TryA_G

Cuts at:      0     712     891 
   Size:        712     179
 
SspI AAT'ATT

Cuts at:      0     120     424     891 
   Size:        120     304     467

  Fragments arranged by size: 

                 467     304     120
 
TatI w'GTAC_w

Cuts at:      0     241     469     891 
   Size:        241     228     422

  Fragments arranged by size: 

                 422     241     228
 
XmnI GAAnn'nnTTC

Cuts at:      0     812     891 
   Size:        812      79
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AlfI    AlwNI     ApoI     BaeI     BanI
     BmrI     BplI    BseRI    BseYI     BsgI     BsmI    BspMI    BsrGI
    BstYI     BtsI   DraIII     EarI     EciI  Eco57MI    EcoRI    Hin4I
   HincII  HindIII     HpaI     KpnI     PfoI     ScaI     SfcI     SspI
     TatI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI    HaeII     KasI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI