Restriction map for
pF1KB6363
(Linear) (Six-base) MAPSORT of: KIBB6363.seq Check: 4307 from: 1 to: 801
KIBB6363.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 801
Size: 358 443
ApaI G_GGCC'C
Cuts at: 0 312 801
Size: 312 489
BamHI G'GATC_C
Cuts at: 0 98 801
Size: 98 703
BanI G'GyrC_C
Cuts at: 0 226 801
Size: 226 575
BanII G_rGCy'C
Cuts at: 0 7 312 371 491 728 801
Size: 7 305 59 120 237 73
Fragments arranged by size:
305 237 120 73 59 7
BbeI G_GCGC'C
Cuts at: 0 230 801
Size: 230 571
BbsI GAAGACnn'nnnn_
Cuts at: 0 165 782 801
Size: 165 617 19
Fragments arranged by size:
617 165 19
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 675 709 801
Size: 675 34 92
Fragments arranged by size:
675 92 34
BlpI GC'TnA_GC
Cuts at: 0 6 485 801
Size: 6 479 316
Fragments arranged by size:
479 316 6
Bme1580I G_kGCm'C
Cuts at: 0 312 801
Size: 312 489
BmgBI CAC'GTC
Cuts at: 0 765 801
Size: 765 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 801
Size: 129 672
Bpu10I CC'TnA_GC
Cuts at: 0 154 434 801
Size: 154 280 367
Fragments arranged by size:
367 280 154
BsaAI yAC'GTr
Cuts at: 0 426 801
Size: 426 375
BsaHI Gr'CG_yC
Cuts at: 0 227 801
Size: 227 574
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 132 801
Size: 132 669
BseYI C'CCAG_C
Cuts at: 0 252 452 801
Size: 252 200 349
Fragments arranged by size:
349 252 200
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 679 801
Size: 448 231 122
Fragments arranged by size:
448 231 122
BsiEI CG_ry'CG
Cuts at: 0 305 801
Size: 305 496
BsiHKAI G_wGCw'C
Cuts at: 0 371 491 728 801
Size: 371 120 237 73
Fragments arranged by size:
371 237 120 73
Bsp1286I G_dGCh'C
Cuts at: 0 7 312 371 491 728 801
Size: 7 305 59 120 237 73
Fragments arranged by size:
305 237 120 73 59 7
BspHI T'CATG_A
Cuts at: 0 491 801
Size: 491 310
BsrDI GCAATG_nn'
Cuts at: 0 287 801
Size: 287 514
BsrFI r'CCGG_y
Cuts at: 0 758 801
Size: 758 43
BstEII G'GTnAC_C
Cuts at: 0 507 801
Size: 507 294
BstYI r'GATC_y
Cuts at: 0 98 801
Size: 98 703
Bsu36I CC'TnA_GG
Cuts at: 0 503 617 801
Size: 503 114 184
Fragments arranged by size:
503 184 114
BtsI GCAGTG_nn'
Cuts at: 0 80 801
Size: 80 721
EaeI y'GGCC_r
Cuts at: 0 104 302 713 801
Size: 104 198 411 88
Fragments arranged by size:
411 198 104 88
EagI C'GGCC_G
Cuts at: 0 302 801
Size: 302 499
EarI CTCTTCn'nnn_
Cuts at: 0 43 49 319 536 801
Size: 43 6 270 217 265
Fragments arranged by size:
270 265 217 43 6
EcoICRI GAG'CTC
Cuts at: 0 369 489 726 801
Size: 369 120 237 75
Fragments arranged by size:
369 237 120 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 358 801
Size: 129 229 443
Fragments arranged by size:
443 229 129
EcoO109I rG'GnC_Cy
Cuts at: 0 164 308 309 582 596 789 801
Size: 164 144 1 273 14 193 12
Fragments arranged by size:
273 193 164 144 14 12 1
HaeII r_GCGC'y
Cuts at: 0 230 801
Size: 230 571
HincII GTy'rAC
Cuts at: 0 234 801
Size: 234 567
KasI G'GCGC_C
Cuts at: 0 226 801
Size: 226 575
MscI TGG'CCA
Cuts at: 0 715 801
Size: 715 86
MslI CAynn'nnrTG
Cuts at: 0 181 801
Size: 181 620
MspA1I CmG'CkG
Cuts at: 0 245 801
Size: 245 556
NaeI GCC'GGC
Cuts at: 0 760 801
Size: 760 41
NarI GG'CG_CC
Cuts at: 0 227 801
Size: 227 574
NgoMIV G'CCGG_C
Cuts at: 0 758 801
Size: 758 43
NruI TCG'CGA
Cuts at: 0 395 801
Size: 395 406
PpuMI rG'GwC_Cy
Cuts at: 0 596 789 801
Size: 596 193 12
Fragments arranged by size:
596 193 12
PspOMI G'GGCC_C
Cuts at: 0 308 801
Size: 308 493
PstI C_TGCA'G
Cuts at: 0 410 674 801
Size: 410 264 127
Fragments arranged by size:
410 264 127
SacI G_AGCT'C
Cuts at: 0 371 491 728 801
Size: 371 120 237 73
Fragments arranged by size:
371 237 120 73
SbfI CC_TGCA'GG
Cuts at: 0 410 801
Size: 410 391
SfcI C'TryA_G
Cuts at: 0 406 670 780 801
Size: 406 264 110 21
Fragments arranged by size:
406 264 110 21
SfoI GGC'GCC
Cuts at: 0 228 801
Size: 228 573
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 697 801
Size: 697 104
TatI w'GTAC_w
Cuts at: 0 260 801
Size: 260 541
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 588 620 754 786 801
Size: 588 32 134 32 15
Fragments arranged by size:
588 134 32 32 15
XmnI GAAnn'nnTTC
Cuts at: 0 571 801
Size: 571 230
Enzymes that do cut:
AcuI ApaI BamHI BanI BanII BbeI BbsI BcgI
BlpI Bme1580I BmgBI BpmI Bpu10I BsaAI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrDI BsrFI
BstEII BstYI Bsu36I BtsI EaeI EagI EarI EcoICRI
Eco57MI EcoO109I HaeII HincII KasI MscI MslI MspA1I
NaeI NarI NgoMIV NruI PpuMI PspOMI PstI SacI
SbfI SfcI SfoI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BbvCI BciVI
BclI BfrBI BglI BglII BmrI BmtI BplI BpuEI
BsaI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HindIII HpaI KpnI MfeI MluI MmeI NcoI
NdeI NheI NotI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI Tth111I
XbaI XcmI XhoI XmaI ZraI