Restriction map for
pF1KB6366
(Linear) (Six-base) MAPSORT of: KIBB6366.seq Check: 1827 from: 1 to: 894
KIBB6366.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 0 392 894
Size: 392 502
ApaLI G'TGCA_C
Cuts at: 0 622 894
Size: 622 272
BanI G'GyrC_C
Cuts at: 0 442 817 894
Size: 442 375 77
Fragments arranged by size:
442 375 77
BanII G_rGCy'C
Cuts at: 0 518 894
Size: 518 376
BbeI G_GCGC'C
Cuts at: 0 446 894
Size: 446 448
BclI T'GATC_A
Cuts at: 0 6 894
Size: 6 888
BglI GCCn_nnn'nGGC
Cuts at: 0 370 894
Size: 370 524
BglII A'GATC_T
Cuts at: 0 488 894
Size: 488 406
Bme1580I G_kGCm'C
Cuts at: 0 626 894
Size: 626 268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 792 894
Size: 231 561 102
Fragments arranged by size:
561 231 102
BsaHI Gr'CG_yC
Cuts at: 0 47 352 443 894
Size: 47 305 91 451
Fragments arranged by size:
451 305 91 47
BsaWI w'CCGG_w
Cuts at: 0 355 894
Size: 355 539
BseYI C'CCAG_C
Cuts at: 0 367 449 894
Size: 367 82 445
Fragments arranged by size:
445 367 82
BsiEI CG_ry'CG
Cuts at: 0 77 729 894
Size: 77 652 165
Fragments arranged by size:
652 165 77
BsiHKAI G_wGCw'C
Cuts at: 0 626 894
Size: 626 268
BsmBI CGTCTCn'nnnn_
Cuts at: 0 66 540 894
Size: 66 474 354
Fragments arranged by size:
474 354 66
Bsp1286I G_dGCh'C
Cuts at: 0 518 626 894
Size: 518 108 268
Fragments arranged by size:
518 268 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 99 894
Size: 99 795
BsrGI T'GTAC_A
Cuts at: 0 749 894
Size: 749 145
BstEII G'GTnAC_C
Cuts at: 0 217 343 894
Size: 217 126 551
Fragments arranged by size:
551 217 126
BstYI r'GATC_y
Cuts at: 0 488 894
Size: 488 406
Bsu36I CC'TnA_GG
Cuts at: 0 185 508 894
Size: 185 323 386
Fragments arranged by size:
386 323 185
BtgI C'CryG_G
Cuts at: 0 71 462 894
Size: 71 391 432
Fragments arranged by size:
432 391 71
EaeI y'GGCC_r
Cuts at: 0 38 224 726 894
Size: 38 186 502 168
Fragments arranged by size:
502 186 168 38
EagI C'GGCC_G
Cuts at: 0 726 894
Size: 726 168
EarI CTCTTCn'nnn_
Cuts at: 0 298 894
Size: 298 596
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 792 894
Size: 231 561 102
Fragments arranged by size:
561 231 102
EcoNI CCTnn'n_nnAGG
Cuts at: 0 210 894
Size: 210 684
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 309 825 894
Size: 309 516 69
Fragments arranged by size:
516 309 69
HaeII r_GCGC'y
Cuts at: 0 333 446 894
Size: 333 113 448
Fragments arranged by size:
448 333 113
HincII GTy'rAC
Cuts at: 0 765 894
Size: 765 129
KasI G'GCGC_C
Cuts at: 0 442 894
Size: 442 452
MscI TGG'CCA
Cuts at: 0 226 894
Size: 226 668
MslI CAynn'nnrTG
Cuts at: 0 631 894
Size: 631 263
MspA1I CmG'CkG
Cuts at: 0 55 73 367 399 894
Size: 55 18 294 32 495
Fragments arranged by size:
495 294 55 32 18
NarI GG'CG_CC
Cuts at: 0 443 894
Size: 443 451
NcoI C'CATG_G
Cuts at: 0 462 894
Size: 462 432
NspI r_CATG'y
Cuts at: 0 121 894
Size: 121 773
PflMI CCAn_nnn'nTGG
Cuts at: 0 468 894
Size: 468 426
PshAI GACnn'nnGTC
Cuts at: 0 73 664 894
Size: 73 591 230
Fragments arranged by size:
591 230 73
PstI C_TGCA'G
Cuts at: 0 110 894
Size: 110 784
SacII CC_GC'GG
Cuts at: 0 74 894
Size: 74 820
SapI GCTCTTCn'nnn_
Cuts at: 0 298 894
Size: 298 596
SexAI A'CCwGG_T
Cuts at: 0 415 894
Size: 415 479
SfcI C'TryA_G
Cuts at: 0 106 558 894
Size: 106 452 336
Fragments arranged by size:
452 336 106
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 370 894
Size: 370 524
SfoI GGC'GCC
Cuts at: 0 444 894
Size: 444 450
SphI G_CATG'C
Cuts at: 0 121 894
Size: 121 773
StuI AGG'CCT
Cuts at: 0 805 894
Size: 805 89
StyI C'CwwG_G
Cuts at: 0 462 543 593 800 894
Size: 462 81 50 207 94
Fragments arranged by size:
462 207 94 81 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 265 894
Size: 265 629
TatI w'GTAC_w
Cuts at: 0 149 281 749 894
Size: 149 132 468 145
Fragments arranged by size:
468 149 145 132
Enzymes that do cut:
AccI ApaLI BanI BanII BbeI BclI BglI BglII
Bme1580I BpmI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrGI BstEII BstYI Bsu36I BtgI EaeI
EagI EarI Eco57MI EcoNI FalI HaeII HincII KasI
MscI MslI MspA1I NarI NcoI NspI PflMI PshAI
PstI SacII SapI SexAI SfcI SfiI SfoI SphI
StuI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbsI
BbvCI BcgI BciVI BfrBI BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI
BsgI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoO109I
EcoRI EcoRV FseI FspI FspAI Hin4I HindIII HpaI
KpnI MfeI MluI MmeI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SalI SanDI SbfI ScaI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI