(Linear) (Six-base) MAPSORT of: KIBB6369.seq  Check: 5824  from: 1  to: 807



KIBB6369.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     275     807 
   Size:         80     195     532

  Fragments arranged by size: 

                 532     195      80
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     263     365     560     807 
   Size:        263     102     195     247

  Fragments arranged by size: 

                 263     247     195     102
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     142     176     807 
   Size:        142      34     631

  Fragments arranged by size: 

                 631     142      34
 
BglII A'GATC_T

Cuts at:      0     306     807 
   Size:        306     501
 
BlpI GC'TnA_GC

Cuts at:      0     164     807 
   Size:        164     643
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     549     777     807 
   Size:        549     228      30

  Fragments arranged by size: 

                 549     228      30
 
BsaBI GATnn'nnATC

Cuts at:      0      17     807 
   Size:         17     790
 
BsaWI w'CCGG_w

Cuts at:      0      21     807 
   Size:         21     786
 
BspEI T'CCGG_A

Cuts at:      0      21     807 
   Size:         21     786
 
BstYI r'GATC_y

Cuts at:      0      12      18     306     771     807 
   Size:         12       6     288     465      36

  Fragments arranged by size: 

                 465     288      36      12       6
 
BtsI GCAGTG_nn'

Cuts at:      0     631     807 
   Size:        631     176
 
EaeI y'GGCC_r

Cuts at:      0     715     807 
   Size:        715      92
 
EarI CTCTTCn'nnn_

Cuts at:      0     278     807 
   Size:        278     529
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     275     807 
   Size:         80     195     532

  Fragments arranged by size: 

                 532     195      80
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      85     378     807 
   Size:         85     293     429

  Fragments arranged by size: 

                 429     293      85
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     291     323     807 
   Size:        291      32     484

  Fragments arranged by size: 

                 484     291      32
 
HindIII A'AGCT_T

Cuts at:      0     794     807 
   Size:        794      13
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     365     799     807 
   Size:        365     434       8

  Fragments arranged by size: 

                 434     365       8
 
MscI TGG'CCA

Cuts at:      0     717     807 
   Size:        717      90
 
MspA1I CmG'CkG

Cuts at:      0     205     807 
   Size:        205     602
 
NdeI CA'TA_TG

Cuts at:      0     690     807 
   Size:        690     117
 
PsiI TTA'TAA

Cuts at:      0     658     807 
   Size:        658     149
 
ScaI AGT'ACT

Cuts at:      0      30     807 
   Size:         30     777
 
SfcI C'TryA_G

Cuts at:      0      32     807 
   Size:         32     775
 
SmlI C'TyrA_G

Cuts at:      0     528     756     807 
   Size:        528     228      51

  Fragments arranged by size: 

                 528     228      51
 
StyI C'CwwG_G

Cuts at:      0     536     807 
   Size:        536     271
 
TatI w'GTAC_w

Cuts at:      0      28     420     807 
   Size:         28     392     387

  Fragments arranged by size: 

                 392     387      28
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     364     396     807 
   Size:        364      32     411

  Fragments arranged by size: 

                 411     364      32
 
XbaI T'CTAG_A

Cuts at:      0      15     549     807 
   Size:         15     534     258

  Fragments arranged by size: 

                 534     258      15
 
XmnI GAAnn'nnTTC

Cuts at:      0     242     260     807 
   Size:        242      18     547

  Fragments arranged by size: 

                 547     242      18
 

 Enzymes that do cut:

     AcuI     BbsI     BcgI    BglII     BlpI    BpuEI    BsaBI    BsaWI
    BspEI    BstYI     BtsI     EaeI     EarI  Eco57MI     FalI    Hin4I
  HindIII     MmeI     MscI   MspA1I     NdeI     PsiI     ScaI     SfcI
     SmlI     StyI     TatI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MslI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII  Tth111I     XcmI     XhoI     XmaI     ZraI