Restriction map for
pF1KB6370
(Linear) (Six-base) MAPSORT of: KIBB6370.seq Check: 519 from: 1 to: 894
KIBB6370.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AatII G_ACGT'C
Cuts at: 0 131 894
Size: 131 763
AccI GT'mk_AC
Cuts at: 0 518 894
Size: 518 376
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 688 894
Size: 250 438 206
Fragments arranged by size:
438 250 206
AhdI GACnn_n'nnGTC
Cuts at: 0 198 894
Size: 198 696
ApaLI G'TGCA_C
Cuts at: 0 709 894
Size: 709 185
ApoI r'AATT_y
Cuts at: 0 436 894
Size: 436 458
AvaI C'yCGr_G
Cuts at: 0 719 894
Size: 719 175
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 171 204 894
Size: 171 33 690
Fragments arranged by size:
690 171 33
BanI G'GyrC_C
Cuts at: 0 12 876 894
Size: 12 864 18
Fragments arranged by size:
864 18 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 131 894
Size: 131 763
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 700 734 894
Size: 700 34 160
Fragments arranged by size:
700 160 34
Bme1580I G_kGCm'C
Cuts at: 0 559 713 812 881 894
Size: 559 154 99 69 13
Fragments arranged by size:
559 154 99 69 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 113 894
Size: 113 781
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 240 565 894
Size: 151 89 325 329
Fragments arranged by size:
329 325 151 89
BsaI GGTCTCn'nnnn_
Cuts at: 0 289 344 894
Size: 289 55 550
Fragments arranged by size:
550 289 55
BsaAI yAC'GTr
Cuts at: 0 345 522 843 894
Size: 345 177 321 51
Fragments arranged by size:
345 321 177 51
BsaHI Gr'CG_yC
Cuts at: 0 128 894
Size: 128 766
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 538 568 730 760 894
Size: 538 30 162 30 134
Fragments arranged by size:
538 162 134 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 562 565 894
Size: 562 3 329
Fragments arranged by size:
562 329 3
BseYI C'CCAG_C
Cuts at: 0 453 474 863 894
Size: 453 21 389 31
Fragments arranged by size:
453 389 31 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 754 865 894
Size: 544 210 111 29
Fragments arranged by size:
544 210 111 29
BsiHKAI G_wGCw'C
Cuts at: 0 713 894
Size: 713 181
BsmI GAATG_Cn'
Cuts at: 0 397 894
Size: 397 497
Bsp1286I G_dGCh'C
Cuts at: 0 559 713 812 881 894
Size: 559 154 99 69 13
Fragments arranged by size:
559 154 99 69 13
BsrFI r'CCGG_y
Cuts at: 0 605 724 894
Size: 605 119 170
Fragments arranged by size:
605 170 119
BssSI C'ACGA_G
Cuts at: 0 871 894
Size: 871 23
BstEII G'GTnAC_C
Cuts at: 0 630 894
Size: 630 264
BstYI r'GATC_y
Cuts at: 0 612 894
Size: 612 282
BtgI C'CryG_G
Cuts at: 0 209 894
Size: 209 685
BtsI GCAGTG_nn'
Cuts at: 0 812 894
Size: 812 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 240 250 565 688 894
Size: 151 89 10 315 123 206
Fragments arranged by size:
315 206 151 123 89 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 547 894
Size: 547 347
EcoO109I rG'GnC_Cy
Cuts at: 0 46 144 462 645 894
Size: 46 98 318 183 249
Fragments arranged by size:
318 249 183 98 46
EcoRI G'AATT_C
Cuts at: 0 436 894
Size: 436 458
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 184 216 604 636 894
Size: 184 32 388 32 258
Fragments arranged by size:
388 258 184 32 32
MslI CAynn'nnrTG
Cuts at: 0 289 894
Size: 289 605
MspA1I CmG'CkG
Cuts at: 0 392 528 894
Size: 392 136 366
Fragments arranged by size:
392 366 136
NaeI GCC'GGC
Cuts at: 0 726 894
Size: 726 168
NcoI C'CATG_G
Cuts at: 0 209 894
Size: 209 685
NgoMIV G'CCGG_C
Cuts at: 0 724 894
Size: 724 170
NspI r_CATG'y
Cuts at: 0 121 894
Size: 121 773
PasI CC'CwG_GG
Cuts at: 0 694 881 894
Size: 694 187 13
Fragments arranged by size:
694 187 13
PfoI T'CCnGG_A
Cuts at: 0 599 894
Size: 599 295
PmlI CAC'GTG
Cuts at: 0 843 894
Size: 843 51
PpuMI rG'GwC_Cy
Cuts at: 0 46 462 894
Size: 46 416 432
Fragments arranged by size:
432 416 46
SanDI GG'GwC_CC
Cuts at: 0 462 894
Size: 462 432
SspI AAT'ATT
Cuts at: 0 409 894
Size: 409 485
StyI C'CwwG_G
Cuts at: 0 147 209 533 894
Size: 147 62 324 361
Fragments arranged by size:
361 324 147 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 783 894
Size: 783 111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 538 570 894
Size: 538 32 324
Fragments arranged by size:
538 324 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 540 894
Size: 540 354
ZraI GAC'GTC
Cuts at: 0 129 894
Size: 129 765
Enzymes that do cut:
AatII AccI AcuI AhdI ApaLI ApoI AvaI BaeI
BanI BbsI BcgI Bme1580I BmrI BpmI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BsrFI BssSI BstEII BstYI BtgI BtsI Eco57MI EcoNI
EcoO109I EcoRI Hin4I MslI MspA1I NaeI NcoI NgoMIV
NspI PasI PfoI PmlI PpuMI SanDI SspI StyI
TaqII TstI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaI AscI AseI AsiSI AvrII
BamHI BanII BbeI BbvCI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaBI
BsaWI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NarI NdeI NheI NotI NruI NsiI
PacI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
StuI SwaI TatI Tth111I XbaI XhoI XmaI XmnI