(Linear) (Six-base) MAPSORT of: KIBB6371.seq  Check: 4464  from: 1  to: 807



KIBB6371.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AflII C'TTAA_G

Cuts at:      0     600     807 
   Size:        600     207
 
AlwNI CAG_nnn'CTG

Cuts at:      0     630     807 
   Size:        630     177
 
ApaI G_GGCC'C

Cuts at:      0     442     807 
   Size:        442     365
 
ApoI r'AATT_y

Cuts at:      0     203     550     807 
   Size:        203     347     257

  Fragments arranged by size: 

                 347     257     203
 
BanII G_rGCy'C

Cuts at:      0      61     442     507     807 
   Size:         61     381      65     300

  Fragments arranged by size: 

                 381     300      65      61
 
BglI GCCn_nnn'nGGC

Cuts at:      0      65     807 
   Size:         65     742
 
BlpI GC'TnA_GC

Cuts at:      0       7     807 
   Size:          7     800
 
Bme1580I G_kGCm'C

Cuts at:      0     442     807 
   Size:        442     365
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     387     415     807 
   Size:        387      28     392

  Fragments arranged by size: 

                 392     387      28
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     601     807 
   Size:        601     206
 
Bsp1286I G_dGCh'C

Cuts at:      0      61     442     507     807 
   Size:         61     381      65     300

  Fragments arranged by size: 

                 381     300      65      61
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     771     783     807 
   Size:        771      12      24

  Fragments arranged by size: 

                 771      24      12
 
BsrDI GCAATG_nn'

Cuts at:      0      98     807 
   Size:         98     709
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     754     807 
   Size:        754      53
 
BstBI TT'CG_AA

Cuts at:      0      24     807 
   Size:         24     783
 
BtsI GCAGTG_nn'

Cuts at:      0     322     807 
   Size:        322     485
 
DraI TTT'AAA

Cuts at:      0     569     807 
   Size:        569     238
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      43     807 
   Size:         43     764
 
EaeI y'GGCC_r

Cuts at:      0     387     807 
   Size:        387     420
 
EcoO109I rG'GnC_Cy

Cuts at:      0     439     627     807 
   Size:        439     188     180

  Fragments arranged by size: 

                 439     188     180
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     688     720     807 
   Size:        688      32      87

  Fragments arranged by size: 

                 688      87      32
 
HincII GTy'rAC

Cuts at:      0     216     807 
   Size:        216     591
 
MfeI C'AATT_G

Cuts at:      0     236     807 
   Size:        236     571
 
NdeI CA'TA_TG

Cuts at:      0     399     542     807 
   Size:        399     143     265

  Fragments arranged by size: 

                 399     265     143
 
PasI CC'CwG_GG

Cuts at:      0      63     807 
   Size:         63     744
 
PfoI T'CCnGG_A

Cuts at:      0     713     807 
   Size:        713      94
 
PpuMI rG'GwC_Cy

Cuts at:      0     627     807 
   Size:        627     180
 
PshAI GACnn'nnGTC

Cuts at:      0     543     807 
   Size:        543     264
 
PspOMI G'GGCC_C

Cuts at:      0     438     807 
   Size:        438     369
 
PstI C_TGCA'G

Cuts at:      0     487     621     780     807 
   Size:        487     134     159      27

  Fragments arranged by size: 

                 487     159     134      27
 
SfcI C'TryA_G

Cuts at:      0     483     617     776     807 
   Size:        483     134     159      31

  Fragments arranged by size: 

                 483     159     134      31
 
SmlI C'TyrA_G

Cuts at:      0     600     807 
   Size:        600     207
 
SpeI A'CTAG_T

Cuts at:      0     576     807 
   Size:        576     231
 
SwaI ATTT'AAAT

Cuts at:      0     569     807 
   Size:        569     238
 

 Enzymes that do cut:

    AflII    AlwNI     ApaI     ApoI    BanII     BglI     BlpI Bme1580I
     BsaI    BsmBI Bsp1286I    BspMI    BsrDI   BstAPI    BstBI     BtsI
     DraI     DrdI     EaeI EcoO109I    Hin4I   HincII     MfeI     NdeI
     PasI     PfoI    PpuMI    PshAI   PspOMI     PstI     SfcI     SmlI
     SpeI     SwaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI   DraIII     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI     PsiI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI