Restriction map for
pF1KB6373
(Linear) (Six-base) MAPSORT of: KIBB6373.seq Check: 2579 from: 1 to: 810
KIBB6373.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AleI CACnn'nnGTG
Cuts at: 0 784 810
Size: 784 26
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 742 810
Size: 742 68
AlwNI CAG_nnn'CTG
Cuts at: 0 330 642 810
Size: 330 312 168
Fragments arranged by size:
330 312 168
ApaI G_GGCC'C
Cuts at: 0 102 144 810
Size: 102 42 666
Fragments arranged by size:
666 102 42
AvaI C'yCGr_G
Cuts at: 0 101 810
Size: 101 709
AvrII C'CTAG_G
Cuts at: 0 604 810
Size: 604 206
BanII G_rGCy'C
Cuts at: 0 102 144 158 810
Size: 102 42 14 652
Fragments arranged by size:
652 102 42 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 214 810
Size: 214 596
BbvCI CC'TCA_GC
Cuts at: 0 665 810
Size: 665 145
BglI GCCn_nnn'nGGC
Cuts at: 0 239 653 810
Size: 239 414 157
Fragments arranged by size:
414 239 157
Bme1580I G_kGCm'C
Cuts at: 0 102 144 810
Size: 102 42 666
Fragments arranged by size:
666 102 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 355 652 810
Size: 355 297 158
Fragments arranged by size:
355 297 158
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 321 810
Size: 321 489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 182 352 402 810
Size: 166 16 170 50 408
Fragments arranged by size:
408 170 166 50 16
Bpu10I CC'TnA_GC
Cuts at: 0 665 810
Size: 665 145
BsaI GGTCTCn'nnnn_
Cuts at: 0 217 295 810
Size: 217 78 515
Fragments arranged by size:
515 217 78
BsaHI Gr'CG_yC
Cuts at: 0 791 810
Size: 791 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 529 559 810
Size: 529 30 251
Fragments arranged by size:
529 251 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 313 592 810
Size: 313 279 218
Fragments arranged by size:
313 279 218
BseYI C'CCAG_C
Cuts at: 0 67 635 750 810
Size: 67 568 115 60
Fragments arranged by size:
568 115 67 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 764 810
Size: 696 68 46
Fragments arranged by size:
696 68 46
BsiHKAI G_wGCw'C
Cuts at: 0 158 781 810
Size: 158 623 29
Fragments arranged by size:
623 158 29
Bsp1286I G_dGCh'C
Cuts at: 0 102 144 158 781 810
Size: 102 42 14 623 29
Fragments arranged by size:
623 102 42 29 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 392 810
Size: 392 418
BssHII G'CGCG_C
Cuts at: 0 78 810
Size: 78 732
BssSI C'ACGA_G
Cuts at: 0 730 810
Size: 730 80
BstXI CCAn_nnnn'nTGG
Cuts at: 0 426 501 810
Size: 426 75 309
Fragments arranged by size:
426 309 75
Bsu36I CC'TnA_GG
Cuts at: 0 296 810
Size: 296 514
BtgI C'CryG_G
Cuts at: 0 785 810
Size: 785 25
BtsI GCAGTG_nn'
Cuts at: 0 689 810
Size: 689 121
EaeI y'GGCC_r
Cuts at: 0 240 270 438 464 810
Size: 240 30 168 26 346
Fragments arranged by size:
346 240 168 30 26
EarI CTCTTCn'nnn_
Cuts at: 0 385 810
Size: 385 425
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 220 310 810
Size: 220 90 500
Fragments arranged by size:
500 220 90
EcoICRI GAG'CTC
Cuts at: 0 156 810
Size: 156 654
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 182 352 402 810
Size: 166 16 170 50 408
Fragments arranged by size:
408 170 166 50 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 148 810
Size: 148 662
EcoO109I rG'GnC_Cy
Cuts at: 0 98 141 810
Size: 98 43 669
Fragments arranged by size:
669 98 43
EcoRV GAT'ATC
Cuts at: 0 801 810
Size: 801 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 56 88 95 127 810
Size: 56 32 7 32 683
Fragments arranged by size:
683 56 32 32 7
HincII GTy'rAC
Cuts at: 0 261 537 810
Size: 261 276 273
Fragments arranged by size:
276 273 261
HindIII A'AGCT_T
Cuts at: 0 497 810
Size: 497 313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 810
Size: 754 56
MscI TGG'CCA
Cuts at: 0 272 466 810
Size: 272 194 344
Fragments arranged by size:
344 272 194
MslI CAynn'nnrTG
Cuts at: 0 784 810
Size: 784 26
MspA1I CmG'CkG
Cuts at: 0 95 327 570 664 810
Size: 95 232 243 94 146
Fragments arranged by size:
243 232 146 95 94
NdeI CA'TA_TG
Cuts at: 0 522 810
Size: 522 288
NspI r_CATG'y
Cuts at: 0 630 810
Size: 630 180
PasI CC'CwG_GG
Cuts at: 0 312 810
Size: 312 498
PflMI CCAn_nnn'nTGG
Cuts at: 0 330 642 810
Size: 330 312 168
Fragments arranged by size:
330 312 168
PspOMI G'GGCC_C
Cuts at: 0 98 140 810
Size: 98 42 670
Fragments arranged by size:
670 98 42
PvuII CAG'CTG
Cuts at: 0 327 810
Size: 327 483
SacI G_AGCT'C
Cuts at: 0 158 810
Size: 158 652
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 653 810
Size: 653 157
SphI G_CATG'C
Cuts at: 0 630 810
Size: 630 180
StuI AGG'CCT
Cuts at: 0 294 810
Size: 294 516
StyI C'CwwG_G
Cuts at: 0 470 604 810
Size: 470 134 206
Fragments arranged by size:
470 206 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 293 810
Size: 293 517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 557 810
Size: 557 253
Enzymes that do cut:
AleI AlfI AlwNI ApaI AvaI AvrII BanII BbsI
BbvCI BglI Bme1580I BmrI BplI BpmI Bpu10I BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BssHII BssSI BstXI Bsu36I BtgI BtsI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV Hin4I HincII
HindIII MmeI MscI MslI MspA1I NdeI NspI PasI
PflMI PspOMI PvuII SacI SfiI SphI StuI StyI
TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AloI ApaLI ApoI AscI AseI
AsiSI BaeI BamHI BanI BbeI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BpuEI BsaAI BsaBI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstYI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoRI
FalI FseI FspI FspAI HaeII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI SwaI TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI