Restriction map for
pF1KB6378
(Linear) (Six-base) MAPSORT of: KIBB6378.seq Check: 9946 from: 1 to: 897
KIBB6378.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 0 845 897
Size: 845 52
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 631 897
Size: 631 266
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 749 781 801 833 897
Size: 749 32 20 32 64
Fragments arranged by size:
749 64 32 32 20
ApaI G_GGCC'C
Cuts at: 0 746 897
Size: 746 151
BanII G_rGCy'C
Cuts at: 0 88 169 409 746 832 897
Size: 88 81 240 337 86 65
Fragments arranged by size:
337 240 88 86 81 65
BclI T'GATC_A
Cuts at: 0 53 387 897
Size: 53 334 510
Fragments arranged by size:
510 334 53
BglI GCCn_nnn'nGGC
Cuts at: 0 452 628 897
Size: 452 176 269
Fragments arranged by size:
452 269 176
BlpI GC'TnA_GC
Cuts at: 0 826 897
Size: 826 71
Bme1580I G_kGCm'C
Cuts at: 0 298 746 897
Size: 298 448 151
Fragments arranged by size:
448 298 151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 717 897
Size: 717 180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 437 897
Size: 89 348 460
Fragments arranged by size:
460 348 89
Bpu10I CC'TnA_GC
Cuts at: 0 87 897
Size: 87 810
BsaI GGTCTCn'nnnn_
Cuts at: 0 497 897
Size: 497 400
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 130 151 160 181 897
Size: 130 21 9 21 716
Fragments arranged by size:
716 130 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 473 476 897
Size: 473 3 421
Fragments arranged by size:
473 421 3
BseYI C'CCAG_C
Cuts at: 0 291 421 587 897
Size: 291 130 166 310
Fragments arranged by size:
310 291 166 130
BsiHKAI G_wGCw'C
Cuts at: 0 88 832 897
Size: 88 744 65
Fragments arranged by size:
744 88 65
Bsp1286I G_dGCh'C
Cuts at: 0 88 169 298 409 746 832 897
Size: 88 81 129 111 337 86 65
Fragments arranged by size:
337 129 111 88 86 81 65
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 623 897
Size: 623 274
BsrBI CCG'CTC
Cuts at: 0 6 897
Size: 6 891
BsrDI GCAATG_nn'
Cuts at: 0 680 897
Size: 680 217
BtgI C'CryG_G
Cuts at: 0 440 559 572 897
Size: 440 119 13 325
Fragments arranged by size:
440 325 119 13
BtsI GCAGTG_nn'
Cuts at: 0 366 897
Size: 366 531
EarI CTCTTCn'nnn_
Cuts at: 0 28 897
Size: 28 869
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 530 897
Size: 530 367
EcoICRI GAG'CTC
Cuts at: 0 86 830 897
Size: 86 744 67
Fragments arranged by size:
744 86 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 437 897
Size: 89 348 460
Fragments arranged by size:
460 348 89
EcoNI CCTnn'n_nnAGG
Cuts at: 0 663 897
Size: 663 234
EcoO109I rG'GnC_Cy
Cuts at: 0 742 743 897
Size: 742 1 154
Fragments arranged by size:
742 154 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 658 897
Size: 658 239
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 220 252 481 513 897
Size: 130 32 58 32 229 32 384
Fragments arranged by size:
384 229 130 58 32 32 32
HincII GTy'rAC
Cuts at: 0 582 897
Size: 582 315
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 298 812 897
Size: 116 182 514 85
Fragments arranged by size:
514 182 116 85
MslI CAynn'nnrTG
Cuts at: 0 227 897
Size: 227 670
MspA1I CmG'CkG
Cuts at: 0 291 301 442 897
Size: 291 10 141 455
Fragments arranged by size:
455 291 141 10
NcoI C'CATG_G
Cuts at: 0 559 572 897
Size: 559 13 325
Fragments arranged by size:
559 325 13
PspOMI G'GGCC_C
Cuts at: 0 742 897
Size: 742 155
PvuII CAG'CTG
Cuts at: 0 291 301 897
Size: 291 10 596
Fragments arranged by size:
596 291 10
SacI G_AGCT'C
Cuts at: 0 88 832 897
Size: 88 744 65
Fragments arranged by size:
744 88 65
SacII CC_GC'GG
Cuts at: 0 443 897
Size: 443 454
SfcI C'TryA_G
Cuts at: 0 842 897
Size: 842 55
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 628 897
Size: 628 269
StuI AGG'CCT
Cuts at: 0 700 795 897
Size: 700 95 102
Fragments arranged by size:
700 102 95
StyI C'CwwG_G
Cuts at: 0 121 559 572 872 897
Size: 121 438 13 300 25
Fragments arranged by size:
438 300 121 25 13
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 406 735 897
Size: 406 329 162
Fragments arranged by size:
406 329 162
Enzymes that do cut:
AccI AlfI AloI ApaI BanII BclI BglI BlpI
Bme1580I BplI BpmI Bpu10I BsaI BsaXI BseRI BseYI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BtgI BtsI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII
MmeI MslI MspA1I NcoI PspOMI PvuII SacI SacII
SfcI SfiI StuI StyI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BfrBI BglII BmgBI BmrI BmtI
BpuEI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EcoRI
EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SalI SanDI SapI
SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI