Restriction map for pF1KB6380
 (Linear) (Six-base) MAPSORT of: KIBB6380.seq  Check: 8559  from: 1  to: 897



KIBB6380.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     437     602     897 
   Size:        437     165     295

  Fragments arranged by size: 

                 437     295     165
 
AleI CACnn'nnGTG

Cuts at:      0     499     897 
   Size:        499     398
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     770     802     897 
   Size:        770      32      95

  Fragments arranged by size: 

                 770      95      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     244     897 
   Size:        244     653
 
ApoI r'AATT_y

Cuts at:      0       7     897 
   Size:          7     890
 
BanI G'GyrC_C

Cuts at:      0     694     897 
   Size:        694     203
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      26     195     897 
   Size:         26     169     702

  Fragments arranged by size: 

                 702     169      26
 
BglII A'GATC_T

Cuts at:      0     870     897 
   Size:        870      27
 
BlpI GC'TnA_GC

Cuts at:      0     880     897 
   Size:        880      17
 
Bme1580I G_kGCm'C

Cuts at:      0     383     897 
   Size:        383     514
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     750     897 
   Size:        750     147
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     742     897 
   Size:        742     155
 
BsaAI yAC'GTr

Cuts at:      0      54     897 
   Size:         54     843
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      52     238     277     424     897 
   Size:         52     186      39     147     473

  Fragments arranged by size: 

                 473     186     147      52      39
 
BsiEI CG_ry'CG

Cuts at:      0     837     897 
   Size:        837      60
 
BsiHKAI G_wGCw'C

Cuts at:      0     718     897 
   Size:        718     179
 
BsmI GAATG_Cn'

Cuts at:      0     170     897 
   Size:        170     727
 
Bsp1286I G_dGCh'C

Cuts at:      0     383     718     897 
   Size:        383     335     179

  Fragments arranged by size: 

                 383     335     179
 
BstYI r'GATC_y

Cuts at:      0     870     897 
   Size:        870      27
 
EaeI y'GGCC_r

Cuts at:      0     782     897 
   Size:        782     115
 
EarI CTCTTCn'nnn_

Cuts at:      0     119     178     209     215     413     449     755     897 
   Size:        119      59      31       6     198      36     306     142

  Fragments arranged by size: 

                 306     198     142     119      59      36      31       6
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     437     602     897 
   Size:        437     165     295

  Fragments arranged by size: 

                 437     295     165
 
EcoRI G'AATT_C

Cuts at:      0       7     897 
   Size:          7     890
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     538     553     570     585     897 
   Size:        538      15      17      15     312

  Fragments arranged by size: 

                 538     312      17      15      15
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      14     897 
   Size:         14     883
 
MscI TGG'CCA

Cuts at:      0     784     897 
   Size:        784     113
 
MslI CAynn'nnrTG

Cuts at:      0     499     897 
   Size:        499     398
 
PstI C_TGCA'G

Cuts at:      0     282     897 
   Size:        282     615
 
SapI GCTCTTCn'nnn_

Cuts at:      0     119     449     897 
   Size:        119     330     448

  Fragments arranged by size: 

                 448     330     119
 
SfcI C'TryA_G

Cuts at:      0     278     897 
   Size:        278     619
 
SmlI C'TyrA_G

Cuts at:      0     721     897 
   Size:        721     176
 
StyI C'CwwG_G

Cuts at:      0     144     497     897 
   Size:        144     353     400

  Fragments arranged by size: 

                 400     353     144
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     851     897 
   Size:        851      46
 
TatI w'GTAC_w

Cuts at:      0     388     897 
   Size:        388     509
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     678     710     897 
   Size:        678      32     187

  Fragments arranged by size: 

                 678     187      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     826     897 
   Size:        826      71
 

 Enzymes that do cut:

     AcuI     AleI     AloI    AlwNI     ApoI     BanI     BbsI    BglII
     BlpI Bme1580I     BmrI    BpuEI    BsaAI    BseRI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BstYI     EaeI     EarI  Eco57MI    EcoRI    Hin4I
     MmeI     MscI     MslI     PstI     SapI     SfcI     SmlI     StyI
    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BmgBI     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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