(Linear) (Six-base) MAPSORT of: KIBB6380.seq Check: 8559 from: 1 to: 897 KIBB6380.orf With 182 enzymes: SgfI * June 26, 2008 10:46 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 437 602 897 Size: 437 165 295 Fragments arranged by size: 437 295 165 AleI CACnn'nnGTG Cuts at: 0 499 897 Size: 499 398 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 770 802 897 Size: 770 32 95 Fragments arranged by size: 770 95 32 AlwNI CAG_nnn'CTG Cuts at: 0 244 897 Size: 244 653 ApoI r'AATT_y Cuts at: 0 7 897 Size: 7 890 BanI G'GyrC_C Cuts at: 0 694 897 Size: 694 203 BbsI GAAGACnn'nnnn_ Cuts at: 0 26 195 897 Size: 26 169 702 Fragments arranged by size: 702 169 26 BglII A'GATC_T Cuts at: 0 870 897 Size: 870 27 BlpI GC'TnA_GC Cuts at: 0 880 897 Size: 880 17 Bme1580I G_kGCm'C Cuts at: 0 383 897 Size: 383 514 BmrI ACTGGGnnnn_n' Cuts at: 0 750 897 Size: 750 147 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 742 897 Size: 742 155 BsaAI yAC'GTr Cuts at: 0 54 897 Size: 54 843 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 52 238 277 424 897 Size: 52 186 39 147 473 Fragments arranged by size: 473 186 147 52 39 BsiEI CG_ry'CG Cuts at: 0 837 897 Size: 837 60 BsiHKAI G_wGCw'C Cuts at: 0 718 897 Size: 718 179 BsmI GAATG_Cn' Cuts at: 0 170 897 Size: 170 727 Bsp1286I G_dGCh'C Cuts at: 0 383 718 897 Size: 383 335 179 Fragments arranged by size: 383 335 179 BstYI r'GATC_y Cuts at: 0 870 897 Size: 870 27 EaeI y'GGCC_r Cuts at: 0 782 897 Size: 782 115 EarI CTCTTCn'nnn_ Cuts at: 0 119 178 209 215 413 449 755 897 Size: 119 59 31 6 198 36 306 142 Fragments arranged by size: 306 198 142 119 59 36 31 6 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 437 602 897 Size: 437 165 295 Fragments arranged by size: 437 295 165 EcoRI G'AATT_C Cuts at: 0 7 897 Size: 7 890 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 538 553 570 585 897 Size: 538 15 17 15 312 Fragments arranged by size: 538 312 17 15 15 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 14 897 Size: 14 883 MscI TGG'CCA Cuts at: 0 784 897 Size: 784 113 MslI CAynn'nnrTG Cuts at: 0 499 897 Size: 499 398 PstI C_TGCA'G Cuts at: 0 282 897 Size: 282 615 SapI GCTCTTCn'nnn_ Cuts at: 0 119 449 897 Size: 119 330 448 Fragments arranged by size: 448 330 119 SfcI C'TryA_G Cuts at: 0 278 897 Size: 278 619 SmlI C'TyrA_G Cuts at: 0 721 897 Size: 721 176 StyI C'CwwG_G Cuts at: 0 144 497 897 Size: 144 353 400 Fragments arranged by size: 400 353 144 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 851 897 Size: 851 46 TatI w'GTAC_w Cuts at: 0 388 897 Size: 388 509 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 678 710 897 Size: 678 32 187 Fragments arranged by size: 678 187 32 XmnI GAAnn'nnTTC Cuts at: 0 826 897 Size: 826 71 Enzymes that do cut: AcuI AleI AloI AlwNI ApoI BanI BbsI BglII BlpI Bme1580I BmrI BpuEI BsaAI BseRI BsiEI BsiHKAI BsmI Bsp1286I BstYI EaeI EarI Eco57MI EcoRI Hin4I MmeI MscI MslI PstI SapI SfcI SmlI StyI TaqII TatI TstI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglI BmgBI BmtI BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI