(Linear) (Six-base) MAPSORT of: KIBB6381.seq  Check: 1509  from: 1  to: 816



KIBB6381.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AclI AA'CG_TT

Cuts at:      0     403     816 
   Size:        403     413
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     570     602     816 
   Size:        570      32     214

  Fragments arranged by size: 

                 570     214      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     757     816 
   Size:        757      59
 
ApoI r'AATT_y

Cuts at:      0     127     339     816 
   Size:        127     212     477

  Fragments arranged by size: 

                 477     212     127
 
AseI AT'TA_AT

Cuts at:      0     526     816 
   Size:        526     290
 
BamHI G'GATC_C

Cuts at:      0     135     816 
   Size:        135     681
 
BanII G_rGCy'C

Cuts at:      0     222     816 
   Size:        222     594
 
BglII A'GATC_T

Cuts at:      0     739     816 
   Size:        739      77
 
BmgBI CAC'GTC

Cuts at:      0      57     816 
   Size:         57     759
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     471     816 
   Size:        471     345
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     734     816 
   Size:        734      82
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     333     816 
   Size:        333     483
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     572     602     701     816 
   Size:        572      30      99     115

  Fragments arranged by size: 

                 572     115      99      30
 
BsaBI GATnn'nnATC

Cuts at:      0      98     816 
   Size:         98     718
 
BseYI C'CCAG_C

Cuts at:      0     391     816 
   Size:        391     425
 
BsmI GAATG_Cn'

Cuts at:      0     480     816 
   Size:        480     336
 
Bsp1286I G_dGCh'C

Cuts at:      0     222     816 
   Size:        222     594
 
BspHI T'CATG_A

Cuts at:      0     380     816 
   Size:        380     436
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     554     816 
   Size:        554     262
 
BsrFI r'CCGG_y

Cuts at:      0     638     816 
   Size:        638     178
 
BssSI C'ACGA_G

Cuts at:      0     227     816 
   Size:        227     589
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     154     816 
   Size:        154     662
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     724     816 
   Size:        724      92
 
BstYI r'GATC_y

Cuts at:      0     135     366     739     816 
   Size:        135     231     373      77

  Fragments arranged by size: 

                 373     231     135      77
 
DraIII CAC_nnn'GTG

Cuts at:      0     232     816 
   Size:        232     584
 
EaeI y'GGCC_r

Cuts at:      0      41     816 
   Size:         41     775
 
EarI CTCTTCn'nnn_

Cuts at:      0     231     706     816 
   Size:        231     475     110

  Fragments arranged by size: 

                 475     231     110
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     734     816 
   Size:        734      82
 
EcoO109I rG'GnC_Cy

Cuts at:      0     773     816 
   Size:        773      43
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      18      26      50     328     360     816 
   Size:         18       8      24     278      32     456

  Fragments arranged by size: 

                 456     278      32      24      18       8
 
HincII GTy'rAC

Cuts at:      0     804     816 
   Size:        804      12
 
MspA1I CmG'CkG

Cuts at:      0      40     359     816 
   Size:         40     319     457

  Fragments arranged by size: 

                 457     319      40
 
NspI r_CATG'y

Cuts at:      0     214     816 
   Size:        214     602
 
PfoI T'CCnGG_A

Cuts at:      0     574     816 
   Size:        574     242
 
PpuMI rG'GwC_Cy

Cuts at:      0     773     816 
   Size:        773      43
 
PvuII CAG'CTG

Cuts at:      0     359     816 
   Size:        359     457
 
SapI GCTCTTCn'nnn_

Cuts at:      0     706     816 
   Size:        706     110
 
SfcI C'TryA_G

Cuts at:      0     186     816 
   Size:        186     630
 
SmlI C'TyrA_G

Cuts at:      0     348     816 
   Size:        348     468
 
StyI C'CwwG_G

Cuts at:      0     139     816 
   Size:        139     677
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     212     816 
   Size:        212     604
 

 Enzymes that do cut:

     AclI     AloI    AlwNI     ApoI     AseI    BamHI    BanII    BglII
    BmgBI     BplI     BpmI    BpuEI     BsaI    BsaBI    BseYI     BsmI
 Bsp1286I    BspHI    BspMI    BsrFI    BssSI   BstAPI    BstXI    BstYI
   DraIII     EaeI     EarI  Eco57MI EcoO109I    Hin4I   HincII   MspA1I
     NspI     PfoI    PpuMI    PvuII     SapI     SfcI     SmlI     StyI
    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I     BmrI     BmtI
   Bpu10I    BsaAI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI    BsrGI   BssHII
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI