Restriction map for
pF1KB6386
(Linear) (Six-base) MAPSORT of: KIBB6386.seq Check: 5315 from: 1 to: 903
KIBB6386.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 448 903
Size: 448 455
AccI GT'mk_AC
Cuts at: 0 658 903
Size: 658 245
AfeI AGC'GCT
Cuts at: 0 130 903
Size: 130 773
AlwNI CAG_nnn'CTG
Cuts at: 0 454 903
Size: 454 449
ApaI G_GGCC'C
Cuts at: 0 441 903
Size: 441 462
ApaLI G'TGCA_C
Cuts at: 0 696 903
Size: 696 207
ApoI r'AATT_y
Cuts at: 0 825 903
Size: 825 78
BanI G'GyrC_C
Cuts at: 0 778 903
Size: 778 125
BanII G_rGCy'C
Cuts at: 0 297 441 903
Size: 297 144 462
Fragments arranged by size:
462 297 144
BbsI GAAGACnn'nnnn_
Cuts at: 0 761 903
Size: 761 142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 687 721 903
Size: 687 34 182
Fragments arranged by size:
687 182 34
Bme1580I G_kGCm'C
Cuts at: 0 441 700 903
Size: 441 259 203
Fragments arranged by size:
441 259 203
BmrI ACTGGGnnnn_n'
Cuts at: 0 77 903
Size: 77 826
BmtI G_CTAG'C
Cuts at: 0 619 903
Size: 619 284
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 697 760 850 903
Size: 412 285 63 90 53
Fragments arranged by size:
412 285 90 63 53
Bpu10I CC'TnA_GC
Cuts at: 0 296 903
Size: 296 607
BsaI GGTCTCn'nnnn_
Cuts at: 0 414 903
Size: 414 489
BsaAI yAC'GTr
Cuts at: 0 42 633 811 903
Size: 42 591 178 92
Fragments arranged by size:
591 178 92 42
BsaWI w'CCGG_w
Cuts at: 0 428 903
Size: 428 475
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 214 805 903
Size: 214 591 98
Fragments arranged by size:
591 214 98
BseYI C'CCAG_C
Cuts at: 0 831 903
Size: 831 72
BsiHKAI G_wGCw'C
Cuts at: 0 150 297 700 716 903
Size: 150 147 403 16 187
Fragments arranged by size:
403 187 150 147 16
Bsp1286I G_dGCh'C
Cuts at: 0 150 297 441 700 716 903
Size: 150 147 144 259 16 187
Fragments arranged by size:
259 187 150 147 144 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 448 903
Size: 448 455
BsrDI GCAATG_nn'
Cuts at: 0 498 903
Size: 498 405
BsrFI r'CCGG_y
Cuts at: 0 137 903
Size: 137 766
BsrGI T'GTAC_A
Cuts at: 0 420 903
Size: 420 483
BstAPI GCAn_nnn'nTGC
Cuts at: 0 454 625 903
Size: 454 171 278
Fragments arranged by size:
454 278 171
BstBI TT'CG_AA
Cuts at: 0 707 903
Size: 707 196
BstXI CCAn_nnnn'nTGG
Cuts at: 0 162 903
Size: 162 741
BstYI r'GATC_y
Cuts at: 0 395 855 903
Size: 395 460 48
Fragments arranged by size:
460 395 48
BstZ17I GTA'TAC
Cuts at: 0 659 903
Size: 659 244
BtgI C'CryG_G
Cuts at: 0 161 903
Size: 161 742
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 459 903
Size: 459 444
BtsI GCAGTG_nn'
Cuts at: 0 878 903
Size: 878 25
EaeI y'GGCC_r
Cuts at: 0 98 903
Size: 98 805
EarI CTCTTCn'nnn_
Cuts at: 0 559 704 903
Size: 559 145 199
Fragments arranged by size:
559 199 145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 181 903
Size: 181 722
EcoICRI GAG'CTC
Cuts at: 0 295 903
Size: 295 608
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 697 760 850 903
Size: 412 285 63 90 53
Fragments arranged by size:
412 285 90 63 53
EcoO109I rG'GnC_Cy
Cuts at: 0 111 438 744 903
Size: 111 327 306 159
Fragments arranged by size:
327 306 159 111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 564 903
Size: 564 339
HaeII r_GCGC'y
Cuts at: 0 132 903
Size: 132 771
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 684 716 903
Size: 684 32 187
Fragments arranged by size:
684 187 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 903
Size: 179 724
MspA1I CmG'CkG
Cuts at: 0 363 534 903
Size: 363 171 369
Fragments arranged by size:
369 363 171
NcoI C'CATG_G
Cuts at: 0 161 903
Size: 161 742
NheI G'CTAG_C
Cuts at: 0 615 903
Size: 615 288
PmlI CAC'GTG
Cuts at: 0 42 903
Size: 42 861
PpuMI rG'GwC_Cy
Cuts at: 0 744 903
Size: 744 159
PspOMI G'GGCC_C
Cuts at: 0 437 903
Size: 437 466
PstI C_TGCA'G
Cuts at: 0 459 903
Size: 459 444
PvuII CAG'CTG
Cuts at: 0 534 903
Size: 534 369
SacI G_AGCT'C
Cuts at: 0 297 903
Size: 297 606
SapI GCTCTTCn'nnn_
Cuts at: 0 704 903
Size: 704 199
SbfI CC_TGCA'GG
Cuts at: 0 459 903
Size: 459 444
SfcI C'TryA_G
Cuts at: 0 455 903
Size: 455 448
SnaBI TAC'GTA
Cuts at: 0 811 903
Size: 811 92
StyI C'CwwG_G
Cuts at: 0 161 903
Size: 161 742
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 731 903
Size: 731 172
TatI w'GTAC_w
Cuts at: 0 420 903
Size: 420 483
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 350 382 903
Size: 350 32 521
Fragments arranged by size:
521 350 32
XmnI GAAnn'nnTTC
Cuts at: 0 826 903
Size: 826 77
Enzymes that do cut:
AarI AccI AfeI AlwNI ApaI ApaLI ApoI BanI
BanII BbsI BcgI Bme1580I BmrI BmtI BpmI Bpu10I
BsaI BsaAI BsaWI BseRI BseYI BsiHKAI Bsp1286I BspMI
BsrDI BsrFI BsrGI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI
EcoO109I FalI HaeII Hin4I MmeI MspA1I NcoI NheI
PmlI PpuMI PspOMI PstI PvuII SacI SapI SbfI
SfcI SnaBI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AloI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BplI BpuEI BsaBI BsaHI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BssHII BssSI BstEII Bsu36I ClaI CspCI DraI DraIII
DrdI EagI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
MslI NaeI NarI NdeI NgoMIV NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaI ZraI