Restriction map for
pF1KB6387
(Linear) (Six-base) MAPSORT of: KIBB6387.seq Check: 3347 from: 1 to: 825
KIBB6387.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AatII G_ACGT'C
Cuts at: 0 784 825
Size: 784 41
AvaI C'yCGr_G
Cuts at: 0 87 825
Size: 87 738
BanII G_rGCy'C
Cuts at: 0 272 825
Size: 272 553
BglII A'GATC_T
Cuts at: 0 480 800 825
Size: 480 320 25
Fragments arranged by size:
480 320 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 309 825
Size: 309 516
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 620 700 792 825
Size: 395 225 80 92 33
Fragments arranged by size:
395 225 92 80 33
BsaI GGTCTCn'nnnn_
Cuts at: 0 335 825
Size: 335 490
BsaHI Gr'CG_yC
Cuts at: 0 781 825
Size: 781 44
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 139 169 332 362 825
Size: 139 30 163 30 463
Fragments arranged by size:
463 163 139 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 825
Size: 109 716
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 161 490 825
Size: 41 120 329 335
Fragments arranged by size:
335 329 120 41
BsiEI CG_ry'CG
Cuts at: 0 154 252 825
Size: 154 98 573
Fragments arranged by size:
573 154 98
BsiHKAI G_wGCw'C
Cuts at: 0 86 181 825
Size: 86 95 644
Fragments arranged by size:
644 95 86
BsmI GAATG_Cn'
Cuts at: 0 171 825
Size: 171 654
Bsp1286I G_dGCh'C
Cuts at: 0 86 181 272 825
Size: 86 95 91 553
Fragments arranged by size:
553 95 91 86
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 18 462 825
Size: 18 444 363
Fragments arranged by size:
444 363 18
BssHII G'CGCG_C
Cuts at: 0 49 51 825
Size: 49 2 774
Fragments arranged by size:
774 49 2
BstEII G'GTnAC_C
Cuts at: 0 237 825
Size: 237 588
BstXI CCAn_nnnn'nTGG
Cuts at: 0 352 567 825
Size: 352 215 258
Fragments arranged by size:
352 258 215
BstYI r'GATC_y
Cuts at: 0 480 800 825
Size: 480 320 25
Fragments arranged by size:
480 320 25
BtsI GCAGTG_nn'
Cuts at: 0 149 282 825
Size: 149 133 543
Fragments arranged by size:
543 149 133
DraI TTT'AAA
Cuts at: 0 600 825
Size: 600 225
DraIII CAC_nnn'GTG
Cuts at: 0 245 825
Size: 245 580
DrdI GACnn_nn'nnGTC
Cuts at: 0 587 825
Size: 587 238
EaeI y'GGCC_r
Cuts at: 0 2 249 492 825
Size: 2 247 243 333
Fragments arranged by size:
333 247 243 2
EagI C'GGCC_G
Cuts at: 0 249 825
Size: 249 576
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 620 700 792 825
Size: 395 225 80 92 33
Fragments arranged by size:
395 225 92 80 33
EcoO109I rG'GnC_Cy
Cuts at: 0 130 825
Size: 130 695
HaeII r_GCGC'y
Cuts at: 0 219 825
Size: 219 606
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 470 502 771 803 825
Size: 470 32 269 32 22
Fragments arranged by size:
470 269 32 32 22
HindIII A'AGCT_T
Cuts at: 0 764 825
Size: 764 61
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 456 825
Size: 378 78 369
Fragments arranged by size:
378 369 78
MscI TGG'CCA
Cuts at: 0 494 825
Size: 494 331
MspA1I CmG'CkG
Cuts at: 0 284 548 825
Size: 284 264 277
Fragments arranged by size:
284 277 264
NotI GC'GGCC_GC
Cuts at: 0 249 825
Size: 249 576
PasI CC'CwG_GG
Cuts at: 0 133 825
Size: 133 692
PflMI CCAn_nnn'nTGG
Cuts at: 0 501 825
Size: 501 324
PvuII CAG'CTG
Cuts at: 0 548 825
Size: 548 277
RsrII CG'GwC_CG
Cuts at: 0 202 825
Size: 202 623
ScaI AGT'ACT
Cuts at: 0 68 825
Size: 68 757
SmaI CCC'GGG
Cuts at: 0 89 825
Size: 89 736
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 291 559 825
Size: 291 268 266
Fragments arranged by size:
291 268 266
TatI w'GTAC_w
Cuts at: 0 66 825
Size: 66 759
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 332 364 825
Size: 332 32 461
Fragments arranged by size:
461 332 32
XmaI C'CCGG_G
Cuts at: 0 87 825
Size: 87 738
ZraI GAC'GTC
Cuts at: 0 782 825
Size: 782 43
Enzymes that do cut:
AatII AvaI BanII BglII BmrI BpmI BsaI BsaHI
BsaXI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BssHII BstEII BstXI BstYI BtsI DraI DraIII DrdI
EaeI EagI Eco57MI EcoO109I HaeII Hin4I HindIII MmeI
MscI MspA1I NotI PasI PflMI PvuII RsrII ScaI
SmaI TaqII TatI TstI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BlpI Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaAI
BsaBI BsaWI BseYI BsiWI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI EarI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NruI NsiI NspI PacI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI SacI SacII SalI SanDI
SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI Tth111I XbaI XcmI XhoI XmnI