Restriction map for
pF1KB6392
(Linear) (Six-base) MAPSORT of: KIBB6392.seq Check: 6694 from: 1 to: 909
KIBB6392.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
Acc65I G'GTAC_C
Cuts at: 0 282 909
Size: 282 627
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 345 377 909
Size: 345 32 532
Fragments arranged by size:
532 345 32
AlwNI CAG_nnn'CTG
Cuts at: 0 114 909
Size: 114 795
ApoI r'AATT_y
Cuts at: 0 319 451 909
Size: 319 132 458
Fragments arranged by size:
458 319 132
AvaI C'yCGr_G
Cuts at: 0 399 909
Size: 399 510
BanI G'GyrC_C
Cuts at: 0 282 909
Size: 282 627
BanII G_rGCy'C
Cuts at: 0 7 540 909
Size: 7 533 369
Fragments arranged by size:
533 369 7
BlpI GC'TnA_GC
Cuts at: 0 6 909
Size: 6 903
Bme1580I G_kGCm'C
Cuts at: 0 391 580 909
Size: 391 189 329
Fragments arranged by size:
391 329 189
BmrI ACTGGGnnnn_n'
Cuts at: 0 574 909
Size: 574 335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 118 652 909
Size: 77 41 534 257
Fragments arranged by size:
534 257 77 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 723 909
Size: 723 186
BsaI GGTCTCn'nnnn_
Cuts at: 0 53 215 495 909
Size: 53 162 280 414
Fragments arranged by size:
414 280 162 53
BsiHKAI G_wGCw'C
Cuts at: 0 533 909
Size: 533 376
BsmI GAATG_Cn'
Cuts at: 0 145 436 909
Size: 145 291 473
Fragments arranged by size:
473 291 145
Bsp1286I G_dGCh'C
Cuts at: 0 7 391 533 540 580 909
Size: 7 384 142 7 40 329
Fragments arranged by size:
384 329 142 40 7 7
BsrDI GCAATG_nn'
Cuts at: 0 612 800 909
Size: 612 188 109
Fragments arranged by size:
612 188 109
BssSI C'ACGA_G
Cuts at: 0 459 909
Size: 459 450
BstAPI GCAn_nnn'nTGC
Cuts at: 0 95 155 164 638 909
Size: 95 60 9 474 271
Fragments arranged by size:
474 271 95 60 9
BtgI C'CryG_G
Cuts at: 0 246 909
Size: 246 663
BtsI GCAGTG_nn'
Cuts at: 0 580 909
Size: 580 329
EaeI y'GGCC_r
Cuts at: 0 732 909
Size: 732 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 118 652 909
Size: 77 41 534 257
Fragments arranged by size:
534 257 77 41
EcoO109I rG'GnC_Cy
Cuts at: 0 251 909
Size: 251 658
KpnI G_GTAC'C
Cuts at: 0 286 909
Size: 286 623
MscI TGG'CCA
Cuts at: 0 734 909
Size: 734 175
MspA1I CmG'CkG
Cuts at: 0 10 186 909
Size: 10 176 723
Fragments arranged by size:
723 176 10
NspI r_CATG'y
Cuts at: 0 183 484 909
Size: 183 301 425
Fragments arranged by size:
425 301 183
PflMI CCAn_nnn'nTGG
Cuts at: 0 675 909
Size: 675 234
PfoI T'CCnGG_A
Cuts at: 0 54 134 909
Size: 54 80 775
Fragments arranged by size:
775 80 54
PpuMI rG'GwC_Cy
Cuts at: 0 251 909
Size: 251 658
PspXI vC'TCGA_Gb
Cuts at: 0 399 909
Size: 399 510
PstI C_TGCA'G
Cuts at: 0 260 909
Size: 260 649
PvuII CAG'CTG
Cuts at: 0 10 186 909
Size: 10 176 723
Fragments arranged by size:
723 176 10
SanDI GG'GwC_CC
Cuts at: 0 251 909
Size: 251 658
SbfI CC_TGCA'GG
Cuts at: 0 260 909
Size: 260 649
SfcI C'TryA_G
Cuts at: 0 256 495 909
Size: 256 239 414
Fragments arranged by size:
414 256 239
SmlI C'TyrA_G
Cuts at: 0 399 738 909
Size: 399 339 171
Fragments arranged by size:
399 339 171
SphI G_CATG'C
Cuts at: 0 484 909
Size: 484 425
StyI C'CwwG_G
Cuts at: 0 806 909
Size: 806 103
TatI w'GTAC_w
Cuts at: 0 176 909
Size: 176 733
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 130 162 404 436 883 909
Size: 130 32 242 32 447 26
Fragments arranged by size:
447 242 130 32 32 26
XhoI C'TCGA_G
Cuts at: 0 399 909
Size: 399 510
Enzymes that do cut:
Acc65I AloI AlwNI ApoI AvaI BanI BanII BlpI
Bme1580I BmrI BpmI BpuEI BsaI BsiHKAI BsmI Bsp1286I
BsrDI BssSI BstAPI BtgI BtsI EaeI Eco57MI EcoO109I
KpnI MscI MspA1I NspI PflMI PfoI PpuMI PspXI
PstI PvuII SanDI SbfI SfcI SmlI SphI StyI
TatI TstI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BmgBI BmtI BplI
Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
MfeI MluI MmeI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI
RsrII SacI SacII SalI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI TaqII Tth111I XbaI XcmI XmaI XmnI
ZraI