Restriction map for
pF1KB6393
(Linear) (Six-base) MAPSORT of: KIBB6393.seq Check: 2015 from: 1 to: 828
KIBB6393.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 0 659 828
Size: 659 169
AhdI GACnn_n'nnGTC
Cuts at: 0 99 828
Size: 99 729
AlwNI CAG_nnn'CTG
Cuts at: 0 456 828
Size: 456 372
AvaI C'yCGr_G
Cuts at: 0 435 631 828
Size: 435 196 197
Fragments arranged by size:
435 197 196
BanII G_rGCy'C
Cuts at: 0 796 828
Size: 796 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 595 828
Size: 595 233
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 61 95 828
Size: 61 34 733
Fragments arranged by size:
733 61 34
BclI T'GATC_A
Cuts at: 0 326 828
Size: 326 502
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 546 621 828
Size: 478 68 75 207
Fragments arranged by size:
478 207 75 68
BsaI GGTCTCn'nnnn_
Cuts at: 0 334 828
Size: 334 494
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 718 748 828
Size: 718 30 80
Fragments arranged by size:
718 80 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 298 618 828
Size: 298 320 210
Fragments arranged by size:
320 298 210
BseYI C'CCAG_C
Cuts at: 0 546 828
Size: 546 282
BsiHKAI G_wGCw'C
Cuts at: 0 512 796 828
Size: 512 284 32
Fragments arranged by size:
512 284 32
BsmBI CGTCTCn'nnnn_
Cuts at: 0 107 828
Size: 107 721
Bsp1286I G_dGCh'C
Cuts at: 0 512 796 828
Size: 512 284 32
Fragments arranged by size:
512 284 32
BspHI T'CATG_A
Cuts at: 0 323 828
Size: 323 505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 372 828
Size: 372 456
BsrDI GCAATG_nn'
Cuts at: 0 695 828
Size: 695 133
BtgI C'CryG_G
Cuts at: 0 243 828
Size: 243 585
BtsI GCAGTG_nn'
Cuts at: 0 557 828
Size: 557 271
DrdI GACnn_nn'nnGTC
Cuts at: 0 310 828
Size: 310 518
EaeI y'GGCC_r
Cuts at: 0 230 828
Size: 230 598
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 118 828
Size: 118 710
EcoICRI GAG'CTC
Cuts at: 0 794 828
Size: 794 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 546 621 828
Size: 478 68 75 207
Fragments arranged by size:
478 207 75 68
EcoO109I rG'GnC_Cy
Cuts at: 0 41 261 462 557 627 828
Size: 41 220 201 95 70 201
Fragments arranged by size:
220 201 201 95 70 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 718 750 828
Size: 85 32 601 32 78
Fragments arranged by size:
601 85 78 32 32
MscI TGG'CCA
Cuts at: 0 232 828
Size: 232 596
MspA1I CmG'CkG
Cuts at: 0 297 828
Size: 297 531
NcoI C'CATG_G
Cuts at: 0 243 828
Size: 243 585
PflMI CCAn_nnn'nTGG
Cuts at: 0 419 456 828
Size: 419 37 372
Fragments arranged by size:
419 372 37
PpuMI rG'GwC_Cy
Cuts at: 0 462 627 828
Size: 462 165 201
Fragments arranged by size:
462 201 165
PstI C_TGCA'G
Cuts at: 0 296 623 828
Size: 296 327 205
Fragments arranged by size:
327 296 205
PvuII CAG'CTG
Cuts at: 0 297 828
Size: 297 531
SacI G_AGCT'C
Cuts at: 0 796 828
Size: 796 32
SexAI A'CCwGG_T
Cuts at: 0 464 828
Size: 464 364
SfcI C'TryA_G
Cuts at: 0 292 619 828
Size: 292 327 209
Fragments arranged by size:
327 292 209
StyI C'CwwG_G
Cuts at: 0 243 828
Size: 243 585
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 212 244 828
Size: 212 32 584
Fragments arranged by size:
584 212 32
Enzymes that do cut:
AccI AhdI AlwNI AvaI BanII BbsI BcgI BclI
BpmI BsaI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrDI BtgI BtsI DrdI EaeI EciI
EcoICRI Eco57MI EcoO109I Hin4I MscI MspA1I NcoI PflMI
PpuMI PstI PvuII SacI SexAI SfcI StyI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsgI BsiEI BsiWI BsmI BspEI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EarI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI