Restriction map for
pF1KB6395
(Linear) (Six-base) MAPSORT of: KIBB6395.seq Check: 8850 from: 1 to: 828
KIBB6395.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AclI AA'CG_TT
Cuts at: 0 191 828
Size: 191 637
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 828
Size: 683 145
AlwNI CAG_nnn'CTG
Cuts at: 0 168 828
Size: 168 660
ApoI r'AATT_y
Cuts at: 0 154 828
Size: 154 674
AvaI C'yCGr_G
Cuts at: 0 29 134 269 828
Size: 29 105 135 559
Fragments arranged by size:
559 135 105 29
BanI G'GyrC_C
Cuts at: 0 214 343 828
Size: 214 129 485
Fragments arranged by size:
485 214 129
BanII G_rGCy'C
Cuts at: 0 27 125 828
Size: 27 98 703
Fragments arranged by size:
703 98 27
BbvCI CC'TCA_GC
Cuts at: 0 276 828
Size: 276 552
BmrI ACTGGGnnnn_n'
Cuts at: 0 387 828
Size: 387 441
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 746 828
Size: 101 645 82
Fragments arranged by size:
645 101 82
Bpu10I CC'TnA_GC
Cuts at: 0 276 828
Size: 276 552
BsaAI yAC'GTr
Cuts at: 0 555 828
Size: 555 273
BsaHI Gr'CG_yC
Cuts at: 0 75 828
Size: 75 753
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 719 749 828
Size: 719 30 79
Fragments arranged by size:
719 79 30
BseYI C'CCAG_C
Cuts at: 0 280 524 828
Size: 280 244 304
Fragments arranged by size:
304 280 244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 787 828
Size: 380 407 41
Fragments arranged by size:
407 380 41
BsiEI CG_ry'CG
Cuts at: 0 43 828
Size: 43 785
BsiHKAI G_wGCw'C
Cuts at: 0 125 828
Size: 125 703
BsmI GAATG_Cn'
Cuts at: 0 146 406 828
Size: 146 260 422
Fragments arranged by size:
422 260 146
Bsp1286I G_dGCh'C
Cuts at: 0 27 125 828
Size: 27 98 703
Fragments arranged by size:
703 98 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 344 806 828
Size: 344 462 22
Fragments arranged by size:
462 344 22
BtgI C'CryG_G
Cuts at: 0 210 596 828
Size: 210 386 232
Fragments arranged by size:
386 232 210
BtsI GCAGTG_nn'
Cuts at: 0 266 828
Size: 266 562
EaeI y'GGCC_r
Cuts at: 0 40 392 681 828
Size: 40 352 289 147
Fragments arranged by size:
352 289 147 40
EagI C'GGCC_G
Cuts at: 0 40 828
Size: 40 788
EarI CTCTTCn'nnn_
Cuts at: 0 659 828
Size: 659 169
EcoICRI GAG'CTC
Cuts at: 0 123 828
Size: 123 705
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 683 746 828
Size: 101 582 63 82
Fragments arranged by size:
582 101 82 63
EcoO109I rG'GnC_Cy
Cuts at: 0 284 538 828
Size: 284 254 290
Fragments arranged by size:
290 284 254
EcoRI G'AATT_C
Cuts at: 0 154 828
Size: 154 674
HaeII r_GCGC'y
Cuts at: 0 7 387 828
Size: 7 380 441
Fragments arranged by size:
441 380 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 719 751 828
Size: 719 32 77
Fragments arranged by size:
719 77 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 828
Size: 491 337
MscI TGG'CCA
Cuts at: 0 394 828
Size: 394 434
MspA1I CmG'CkG
Cuts at: 0 39 280 828
Size: 39 241 548
Fragments arranged by size:
548 241 39
NcoI C'CATG_G
Cuts at: 0 210 828
Size: 210 618
PasI CC'CwG_GG
Cuts at: 0 168 828
Size: 168 660
PflMI CCAn_nnn'nTGG
Cuts at: 0 168 828
Size: 168 660
PfoI T'CCnGG_A
Cuts at: 0 540 828
Size: 540 288
PpuMI rG'GwC_Cy
Cuts at: 0 284 538 828
Size: 284 254 290
Fragments arranged by size:
290 284 254
PvuII CAG'CTG
Cuts at: 0 280 828
Size: 280 548
SacI G_AGCT'C
Cuts at: 0 125 828
Size: 125 703
ScaI AGT'ACT
Cuts at: 0 376 828
Size: 376 452
SfcI C'TryA_G
Cuts at: 0 96 828
Size: 96 732
SmaI CCC'GGG
Cuts at: 0 136 828
Size: 136 692
StuI AGG'CCT
Cuts at: 0 670 828
Size: 670 158
StyI C'CwwG_G
Cuts at: 0 210 395 678 828
Size: 210 185 283 150
Fragments arranged by size:
283 210 185 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 160 756 828
Size: 160 596 72
Fragments arranged by size:
596 160 72
TatI w'GTAC_w
Cuts at: 0 374 410 781 828
Size: 374 36 371 47
Fragments arranged by size:
374 371 47 36
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 169 828
Size: 169 659
XmaI C'CCGG_G
Cuts at: 0 134 828
Size: 134 694
Enzymes that do cut:
AclI AcuI AlwNI ApoI AvaI BanI BanII BbvCI
BmrI BpmI Bpu10I BsaAI BsaHI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BtgI BtsI EaeI
EagI EarI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I
MmeI MscI MspA1I NcoI PasI PflMI PfoI PpuMI
PvuII SacI ScaI SfcI SmaI StuI StyI TaqII
TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI
BplI BpuEI BsaI BsaBI BsaWI BseRI BsiWI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EciI EcoNI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MslI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XhoI XmnI
ZraI