Restriction map for
pF1KB6396
(Linear) (Six-base) MAPSORT of: KIBB6396.seq Check: 5884 from: 1 to: 912
KIBB6396.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 749 912
Size: 749 163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 912
Size: 518 394
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 193 767 912
Size: 193 574 145
Fragments arranged by size:
574 193 145
ApaLI G'TGCA_C
Cuts at: 0 797 912
Size: 797 115
ApoI r'AATT_y
Cuts at: 0 108 540 623 912
Size: 108 432 83 289
Fragments arranged by size:
432 289 108 83
BanI G'GyrC_C
Cuts at: 0 305 912
Size: 305 607
BanII G_rGCy'C
Cuts at: 0 328 912
Size: 328 584
BbsI GAAGACnn'nnnn_
Cuts at: 0 851 912
Size: 851 61
BfrBI ATG'CAT
Cuts at: 0 349 912
Size: 349 563
BglI GCCn_nnn'nGGC
Cuts at: 0 598 616 912
Size: 598 18 296
Fragments arranged by size:
598 296 18
BlpI GC'TnA_GC
Cuts at: 0 322 912
Size: 322 590
Bme1580I G_kGCm'C
Cuts at: 0 801 862 912
Size: 801 61 50
Fragments arranged by size:
801 61 50
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 75 912
Size: 75 837
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 912
Size: 577 335
BsaBI GATnn'nnATC
Cuts at: 0 238 912
Size: 238 674
BsaWI w'CCGG_w
Cuts at: 0 422 912
Size: 422 490
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 477 507 780 810 912
Size: 477 30 273 30 102
Fragments arranged by size:
477 273 102 30 30
BsiHKAI G_wGCw'C
Cuts at: 0 182 206 328 801 912
Size: 182 24 122 473 111
Fragments arranged by size:
473 182 122 111 24
Bsp1286I G_dGCh'C
Cuts at: 0 182 206 328 801 862 912
Size: 182 24 122 473 61 50
Fragments arranged by size:
473 182 122 61 50 24
BspEI T'CCGG_A
Cuts at: 0 422 912
Size: 422 490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 749 912
Size: 749 163
BsrDI GCAATG_nn'
Cuts at: 0 430 912
Size: 430 482
BtgI C'CryG_G
Cuts at: 0 699 912
Size: 699 213
BtsI GCAGTG_nn'
Cuts at: 0 545 659 912
Size: 545 114 253
Fragments arranged by size:
545 253 114
EarI CTCTTCn'nnn_
Cuts at: 0 74 512 808 912
Size: 74 438 296 104
Fragments arranged by size:
438 296 104 74
EcoICRI GAG'CTC
Cuts at: 0 326 912
Size: 326 586
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 912
Size: 518 394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 523 912
Size: 523 389
HaeII r_GCGC'y
Cuts at: 0 360 912
Size: 360 552
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 477 509 912
Size: 477 32 403
Fragments arranged by size:
477 403 32
MfeI C'AATT_G
Cuts at: 0 113 313 912
Size: 113 200 599
Fragments arranged by size:
599 200 113
NcoI C'CATG_G
Cuts at: 0 699 912
Size: 699 213
NsiI A_TGCA'T
Cuts at: 0 351 912
Size: 351 561
PflMI CCAn_nnn'nTGG
Cuts at: 0 342 912
Size: 342 570
PstI C_TGCA'G
Cuts at: 0 720 746 912
Size: 720 26 166
Fragments arranged by size:
720 166 26
SacI G_AGCT'C
Cuts at: 0 328 912
Size: 328 584
SapI GCTCTTCn'nnn_
Cuts at: 0 512 912
Size: 512 400
SfcI C'TryA_G
Cuts at: 0 716 742 912
Size: 716 26 170
Fragments arranged by size:
716 170 26
SmlI C'TyrA_G
Cuts at: 0 556 912
Size: 556 356
StuI AGG'CCT
Cuts at: 0 673 912
Size: 673 239
StyI C'CwwG_G
Cuts at: 0 699 912
Size: 699 213
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 780 812 912
Size: 780 32 100
Fragments arranged by size:
780 100 32
Enzymes that do cut:
AarI AcuI AlfI ApaLI ApoI BanI BanII BbsI
BfrBI BglI BlpI Bme1580I BplI BpuEI BsaBI BsaWI
BsaXI BsiHKAI Bsp1286I BspEI BspMI BsrDI BtgI BtsI
EarI EcoICRI Eco57MI FalI HaeII Hin4I MfeI NcoI
NsiI PflMI PstI SacI SapI SfcI SmlI StuI
StyI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BglII BmgBI BmrI BmtI BpmI Bpu10I BsaI
BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiWI BsmI
BsmBI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoNI
EcoO109I EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI