(Linear) (Six-base) MAPSORT of: KIBB6401.seq  Check: 5927  from: 1  to: 912



KIBB6401.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     206     422     912 
   Size:         71     135     216     490

  Fragments arranged by size: 

                 490     216     135      71
 
ApoI r'AATT_y

Cuts at:      0     197     239     451     912 
   Size:        197      42     212     461

  Fragments arranged by size: 

                 461     212     197      42
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     578     611     912 
   Size:        578      33     301

  Fragments arranged by size: 

                 578     301      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     677     912 
   Size:        677     235
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     379     413     912 
   Size:        379      34     499

  Fragments arranged by size: 

                 499     379      34
 
BfrBI ATG'CAT

Cuts at:      0      42     912 
   Size:         42     870
 
BglII A'GATC_T

Cuts at:      0     733     912 
   Size:        733     179
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     605     912 
   Size:        605     307
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     590     704     794     912 
   Size:        590     114      90     118

  Fragments arranged by size: 

                 590     118     114      90
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     575     912 
   Size:        575     337
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     323     912 
   Size:        323     589
 
BsrDI GCAATG_nn'

Cuts at:      0     708     912 
   Size:        708     204
 
BstYI r'GATC_y

Cuts at:      0     733     912 
   Size:        733     179
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     627     912 
   Size:        627     285
 
EaeI y'GGCC_r

Cuts at:      0     423     912 
   Size:        423     489
 
EarI CTCTTCn'nnn_

Cuts at:      0     398     912 
   Size:        398     514
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     206     422     590     704     794     912 
   Size:         71     135     216     168     114      90     118

  Fragments arranged by size: 

                 216     168     135     118     114      90      71
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     608     912 
   Size:        608     304
 
EcoO109I rG'GnC_Cy

Cuts at:      0     753     912 
   Size:        753     159
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     340     372     681     713     805     837     912 
   Size:        340      32     309      32      92      32      75

  Fragments arranged by size: 

                 340     309      92      75      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0      53     912 
   Size:         53     859
 
MfeI C'AATT_G

Cuts at:      0     273     419     912 
   Size:        273     146     493

  Fragments arranged by size: 

                 493     273     146
 
MscI TGG'CCA

Cuts at:      0     425     912 
   Size:        425     487
 
NdeI CA'TA_TG

Cuts at:      0     340     912 
   Size:        340     572
 
NsiI A_TGCA'T

Cuts at:      0      44     912 
   Size:         44     868
 
PpuMI rG'GwC_Cy

Cuts at:      0     753     912 
   Size:        753     159
 
SmlI C'TyrA_G

Cuts at:      0     590     912 
   Size:        590     322
 
TatI w'GTAC_w

Cuts at:      0     100     558     912 
   Size:        100     458     354

  Fragments arranged by size: 

                 458     354     100
 
XbaI T'CTAG_A

Cuts at:      0     243     456     912 
   Size:        243     213     456

  Fragments arranged by size: 

                 456     243     213
 

 Enzymes that do cut:

     AcuI     ApoI     BaeI     BbsI     BcgI    BfrBI    BglII     BplI
     BpmI    BpuEI     BsgI    BsrDI    BstYI     DrdI     EaeI     EarI
  Eco57MI    EcoNI EcoO109I    Hin4I  HindIII     MfeI     MscI     NdeI
     NsiI    PpuMI     SmlI     TatI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI    BbvCI    BciVI     BclI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EagI     EciI
  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MluI     MmeI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI