(Linear) (Six-base) MAPSORT of: KIBB6403.seq  Check: 6694  from: 1  to: 912



KIBB6403.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     257     912 
   Size:        257     655
 
AfeI AGC'GCT

Cuts at:      0     897     912 
   Size:        897      15
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     126     912 
   Size:        126     786
 
AlwNI CAG_nnn'CTG

Cuts at:      0     235     625     912 
   Size:        235     390     287

  Fragments arranged by size: 

                 390     287     235
 
ApoI r'AATT_y

Cuts at:      0     502     912 
   Size:        502     410
 
BamHI G'GATC_C

Cuts at:      0     342     912 
   Size:        342     570
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     618     912 
   Size:        618     294
 
BglII A'GATC_T

Cuts at:      0     540     912 
   Size:        540     372
 
BlpI GC'TnA_GC

Cuts at:      0     818     912 
   Size:        818      94
 
BmtI G_CTAG'C

Cuts at:      0     650     912 
   Size:        650     262
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     782     912 
   Size:        782     130
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     689     912 
   Size:        689     223
 
BsaWI w'CCGG_w

Cuts at:      0     872     912 
   Size:        872      40
 
BsmI GAATG_Cn'

Cuts at:      0     906     912 
   Size:        906       6
 
BspEI T'CCGG_A

Cuts at:      0     872     912 
   Size:        872      40
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     762     912 
   Size:        762     150
 
BstYI r'GATC_y

Cuts at:      0     342     540     912 
   Size:        342     198     372

  Fragments arranged by size: 

                 372     342     198
 
BtsI GCAGTG_nn'

Cuts at:      0     317     488     912 
   Size:        317     171     424

  Fragments arranged by size: 

                 424     317     171
 
EarI CTCTTCn'nnn_

Cuts at:      0     896     912 
   Size:        896      16
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     257     782     912 
   Size:        257     525     130

  Fragments arranged by size: 

                 525     257     130
 
EcoO109I rG'GnC_Cy

Cuts at:      0     751     771     912 
   Size:        751      20     141

  Fragments arranged by size: 

                 751     141      20
 
HaeII r_GCGC'y

Cuts at:      0     899     912 
   Size:        899      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     645     677     912 
   Size:        645      32     235

  Fragments arranged by size: 

                 645     235      32
 
MfeI C'AATT_G

Cuts at:      0     440     912 
   Size:        440     472
 
MslI CAynn'nnrTG

Cuts at:      0     676     912 
   Size:        676     236
 
NheI G'CTAG_C

Cuts at:      0     646     912 
   Size:        646     266
 
PpuMI rG'GwC_Cy

Cuts at:      0     751     912 
   Size:        751     161
 
PstI C_TGCA'G

Cuts at:      0     312     912 
   Size:        312     600
 
SapI GCTCTTCn'nnn_

Cuts at:      0     896     912 
   Size:        896      16
 
SexAI A'CCwGG_T

Cuts at:      0      17     912 
   Size:         17     895
 
SfcI C'TryA_G

Cuts at:      0     308     617     912 
   Size:        308     309     295

  Fragments arranged by size: 

                 309     308     295
 
SmlI C'TyrA_G

Cuts at:      0     704     912 
   Size:        704     208
 
StuI AGG'CCT

Cuts at:      0     463     912 
   Size:        463     449
 
TatI w'GTAC_w

Cuts at:      0     452     912 
   Size:        452     460
 

 Enzymes that do cut:

     AcuI     AfeI     AlfI    AlwNI     ApoI    BamHI    BciVI    BglII
     BlpI     BmtI     BpmI    BpuEI    BsaWI     BsmI    BspEI    BspMI
    BstYI     BtsI     EarI  Eco57MI EcoO109I    HaeII    Hin4I     MfeI
     MslI     NheI    PpuMI     PstI     SapI    SexAI     SfcI     SmlI
     StuI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI     BclI    BfrBI     BglI Bme1580I    BmgBI     BmrI     BplI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI