(Linear) (Six-base) MAPSORT of: KIBB6404.seq  Check: 4530  from: 1  to: 1002



KIBB6404.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394     637     733     766     847    1002 
   Size:        394     243      96      33      81     155

  Fragments arranged by size: 

                 394     243     155      96      81      33
 
AlwNI CAG_nnn'CTG

Cuts at:      0     615    1002 
   Size:        615     387
 
ApoI r'AATT_y

Cuts at:      0     154     209     232    1002 
   Size:        154      55      23     770

  Fragments arranged by size: 

                 770     154      55      23
 
AvaI C'yCGr_G

Cuts at:      0      22     351    1002 
   Size:         22     329     651

  Fragments arranged by size: 

                 651     329      22
 
BamHI G'GATC_C

Cuts at:      0     439     513     547    1002 
   Size:        439      74      34     455

  Fragments arranged by size: 

                 455     439      74      34
 
BanII G_rGCy'C

Cuts at:      0     352    1002 
   Size:        352     650
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     625     754    1002 
   Size:        625     129     248

  Fragments arranged by size: 

                 625     248     129
 
BmgBI CAC'GTC

Cuts at:      0     345    1002 
   Size:        345     657
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     411    1002 
   Size:        411     591
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     475    1002 
   Size:        475     527
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      19     583    1002 
   Size:         19     564     419

  Fragments arranged by size: 

                 564     419      19
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25    1002 
   Size:         25     977
 
BseYI C'CCAG_C

Cuts at:      0     261    1002 
   Size:        261     741
 
BsiEI CG_ry'CG

Cuts at:      0     527    1002 
   Size:        527     475
 
BsiHKAI G_wGCw'C

Cuts at:      0      45     352    1002 
   Size:         45     307     650

  Fragments arranged by size: 

                 650     307      45
 
Bsp1286I G_dGCh'C

Cuts at:      0      45     352    1002 
   Size:         45     307     650

  Fragments arranged by size: 

                 650     307      45
 
BsrGI T'GTAC_A

Cuts at:      0     879    1002 
   Size:        879     123
 
BstYI r'GATC_y

Cuts at:      0     439     513     547    1002 
   Size:        439      74      34     455

  Fragments arranged by size: 

                 455     439      74      34
 
Bsu36I CC'TnA_GG

Cuts at:      0     603    1002 
   Size:        603     399
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     930    1002 
   Size:        930      72
 
BtsI GCAGTG_nn'

Cuts at:      0     146    1002 
   Size:        146     856
 
ClaI AT'CG_AT

Cuts at:      0     629    1002 
   Size:        629     373
 
EaeI y'GGCC_r

Cuts at:      0     126     524     689     759    1002 
   Size:        126     398     165      70     243

  Fragments arranged by size: 

                 398     243     165     126      70
 
EagI C'GGCC_G

Cuts at:      0     524    1002 
   Size:        524     478
 
EarI CTCTTCn'nnn_

Cuts at:      0     985    1002 
   Size:        985      17
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      54    1002 
   Size:         54     948
 
EcoICRI GAG'CTC

Cuts at:      0     350    1002 
   Size:        350     652
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394     637     733     766     847    1002 
   Size:        394     243      96      33      81     155

  Fragments arranged by size: 

                 394     243     155      96      81      33
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     334     366     505     537     586     618    1002 
   Size:        334      32     139      32      49      32     384

  Fragments arranged by size: 

                 384     334     139      49      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     446    1002 
   Size:        446     556
 
MscI TGG'CCA

Cuts at:      0     128     761    1002 
   Size:        128     633     241

  Fragments arranged by size: 

                 633     241     128
 
MslI CAynn'nnrTG

Cuts at:      0     767     902    1002 
   Size:        767     135     100

  Fragments arranged by size: 

                 767     135     100
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     664     696    1002 
   Size:        664      32     306

  Fragments arranged by size: 

                 664     306      32
 
SacI G_AGCT'C

Cuts at:      0     352    1002 
   Size:        352     650
 
SfcI C'TryA_G

Cuts at:      0     726    1002 
   Size:        726     276
 
SmlI C'TyrA_G

Cuts at:      0     490    1002 
   Size:        490     512
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      43     944    1002 
   Size:         43     901      58

  Fragments arranged by size: 

                 901      58      43
 
TatI w'GTAC_w

Cuts at:      0     879    1002 
   Size:        879     123
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     AvaI    BamHI    BanII     BbsI    BmgBI
     BmrI    BpuEI     BsaI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I
    BsrGI    BstYI   Bsu36I    BtgZI     BtsI     ClaI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    Hin4I     MmeI     MscI     MslI
     PsrI     SacI     SfcI     SmlI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmtI
     BplI     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I     BtgI    CspCI     DraI   DraIII     DrdI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI