Restriction map for
pF1KB6405
(Linear) (Six-base) MAPSORT of: KIBB6405.seq Check: 63 from: 1 to: 915
KIBB6405.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AatII G_ACGT'C
Cuts at: 0 737 915
Size: 737 178
AccI GT'mk_AC
Cuts at: 0 449 818 915
Size: 449 369 97
Fragments arranged by size:
449 369 97
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 915
Size: 611 304
AleI CACnn'nnGTG
Cuts at: 0 529 915
Size: 529 386
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 357 915
Size: 357 558
AlwNI CAG_nnn'CTG
Cuts at: 0 73 132 915
Size: 73 59 783
Fragments arranged by size:
783 73 59
BanII G_rGCy'C
Cuts at: 0 212 899 915
Size: 212 687 16
Fragments arranged by size:
687 212 16
BbvCI CC'TCA_GC
Cuts at: 0 63 879 915
Size: 63 816 36
Fragments arranged by size:
816 63 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 808 842 915
Size: 808 34 73
Fragments arranged by size:
808 73 34
BglI GCCn_nnn'nGGC
Cuts at: 0 427 915
Size: 427 488
BglII A'GATC_T
Cuts at: 0 336 570 915
Size: 336 234 345
Fragments arranged by size:
345 336 234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 375 915
Size: 112 263 540
Fragments arranged by size:
540 263 112
Bpu10I CC'TnA_GC
Cuts at: 0 63 879 915
Size: 63 816 36
Fragments arranged by size:
816 63 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 915
Size: 754 161
BsaHI Gr'CG_yC
Cuts at: 0 734 915
Size: 734 181
BsaWI w'CCGG_w
Cuts at: 0 80 915
Size: 80 835
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 373 403 796 826 915
Size: 373 30 393 30 89
Fragments arranged by size:
393 373 89 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 171 817 915
Size: 171 646 98
Fragments arranged by size:
646 171 98
BsiHKAI G_wGCw'C
Cuts at: 0 212 899 915
Size: 212 687 16
Fragments arranged by size:
687 212 16
Bsp1286I G_dGCh'C
Cuts at: 0 212 899 915
Size: 212 687 16
Fragments arranged by size:
687 212 16
BspEI T'CCGG_A
Cuts at: 0 80 915
Size: 80 835
BspHI T'CATG_A
Cuts at: 0 275 915
Size: 275 640
BsrBI CCG'CTC
Cuts at: 0 285 915
Size: 285 630
BstAPI GCAn_nnn'nTGC
Cuts at: 0 73 915
Size: 73 842
BstXI CCAn_nnnn'nTGG
Cuts at: 0 642 915
Size: 642 273
BstYI r'GATC_y
Cuts at: 0 48 336 570 915
Size: 48 288 234 345
Fragments arranged by size:
345 288 234 48
BstZ17I GTA'TAC
Cuts at: 0 450 915
Size: 450 465
BtgI C'CryG_G
Cuts at: 0 422 811 915
Size: 422 389 104
Fragments arranged by size:
422 389 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 905 915
Size: 905 10
BtsI GCAGTG_nn'
Cuts at: 0 74 197 824 915
Size: 74 123 627 91
Fragments arranged by size:
627 123 91 74
EaeI y'GGCC_r
Cuts at: 0 428 491 915
Size: 428 63 424
Fragments arranged by size:
428 424 63
EarI CTCTTCn'nnn_
Cuts at: 0 97 217 915
Size: 97 120 698
Fragments arranged by size:
698 120 97
EcoICRI GAG'CTC
Cuts at: 0 210 897 915
Size: 210 687 18
Fragments arranged by size:
687 210 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 375 611 915
Size: 112 263 236 304
Fragments arranged by size:
304 263 236 112
EcoNI CCTnn'n_nnAGG
Cuts at: 0 799 915
Size: 799 116
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 589 915
Size: 589 326
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 46 78 126 158 779 811 915
Size: 46 32 48 32 621 32 104
Fragments arranged by size:
621 104 48 46 32 32 32
HincII GTy'rAC
Cuts at: 0 819 915
Size: 819 96
MscI TGG'CCA
Cuts at: 0 493 915
Size: 493 422
MslI CAynn'nnrTG
Cuts at: 0 529 915
Size: 529 386
PfoI T'CCnGG_A
Cuts at: 0 410 915
Size: 410 505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 284 316 915
Size: 284 32 599
Fragments arranged by size:
599 284 32
SacI G_AGCT'C
Cuts at: 0 212 899 915
Size: 212 687 16
Fragments arranged by size:
687 212 16
SalI G'TCGA_C
Cuts at: 0 817 915
Size: 817 98
SapI GCTCTTCn'nnn_
Cuts at: 0 217 915
Size: 217 698
SmlI C'TyrA_G
Cuts at: 0 769 915
Size: 769 146
SspI AAT'ATT
Cuts at: 0 374 915
Size: 374 541
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 373 405 796 828 915
Size: 373 32 391 32 87
Fragments arranged by size:
391 373 87 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 296 915
Size: 296 619
ZraI GAC'GTC
Cuts at: 0 735 915
Size: 735 180
Enzymes that do cut:
AatII AccI AcuI AleI AlfI AlwNI BanII BbvCI
BcgI BglI BglII BpmI Bpu10I BpuEI BsaHI BsaWI
BsaXI BseRI BsiHKAI Bsp1286I BspEI BspHI BsrBI BstAPI
BstXI BstYI BstZ17I BtgI BtgZI BtsI EaeI EarI
EcoICRI Eco57MI EcoNI FalI Hin4I HincII MscI MslI
PfoI PpiI SacI SalI SapI SmlI SspI TstI
XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbsI BciVI BclI
BfrBI BlpI Bme1580I BmgBI BmrI BmtI BplI BsaI
BsaAI BsaBI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspMI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
Bsu36I ClaI CspCI DraI DraIII DrdI EagI EciI
EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacII SanDI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI StyI SwaI TaqII TatI Tth111I XbaI XcmI
XhoI XmaI