(Linear) (Six-base) MAPSORT of: KIBB6407.seq Check: 7415 from: 1 to: 918 KIBB6407.orf With 182 enzymes: SgfI * October 7, 2009 14:20 .. AatII G_ACGT'C Cuts at: 0 506 918 Size: 506 412 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 892 918 Size: 892 26 AfeI AGC'GCT Cuts at: 0 706 918 Size: 706 212 AleI CACnn'nnGTG Cuts at: 0 778 918 Size: 778 140 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 796 918 Size: 796 122 ApoI r'AATT_y Cuts at: 0 130 918 Size: 130 788 AvaI C'yCGr_G Cuts at: 0 61 638 918 Size: 61 577 280 Fragments arranged by size: 577 280 61 BamHI G'GATC_C Cuts at: 0 44 918 Size: 44 874 BanI G'GyrC_C Cuts at: 0 780 918 Size: 780 138 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 42 76 918 Size: 42 34 842 Fragments arranged by size: 842 42 34 BglII A'GATC_T Cuts at: 0 288 907 918 Size: 288 619 11 Fragments arranged by size: 619 288 11 BlpI GC'TnA_GC Cuts at: 0 541 769 918 Size: 541 228 149 Fragments arranged by size: 541 228 149 Bme1580I G_kGCm'C Cuts at: 0 785 918 Size: 785 133 BmgBI CAC'GTC Cuts at: 0 565 918 Size: 565 353 BmrI ACTGGGnnnn_n' Cuts at: 0 395 918 Size: 395 523 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 541 918 Size: 541 377 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 145 304 721 918 Size: 145 159 417 197 Fragments arranged by size: 417 197 159 145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 313 918 Size: 313 605 BsaAI yAC'GTr Cuts at: 0 39 575 918 Size: 39 536 343 Fragments arranged by size: 536 343 39 BsaHI Gr'CG_yC Cuts at: 0 57 503 918 Size: 57 446 415 Fragments arranged by size: 446 415 57 BsaWI w'CCGG_w Cuts at: 0 47 918 Size: 47 871 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 247 277 588 618 918 Size: 247 30 311 30 300 Fragments arranged by size: 311 300 247 30 30 BsiHKAI G_wGCw'C Cuts at: 0 775 917 918 Size: 775 142 1 Fragments arranged by size: 775 142 1 BsmBI CGTCTCn'nnnn_ Cuts at: 0 635 918 Size: 635 283 Bsp1286I G_dGCh'C Cuts at: 0 775 785 917 918 Size: 775 10 132 1 Fragments arranged by size: 775 132 10 1 BspEI T'CCGG_A Cuts at: 0 47 918 Size: 47 871 BsrBI CCG'CTC Cuts at: 0 756 918 Size: 756 162 BsrGI T'GTAC_A Cuts at: 0 450 918 Size: 450 468 BssSI C'ACGA_G Cuts at: 0 700 918 Size: 700 218 BstYI r'GATC_y Cuts at: 0 44 288 907 918 Size: 44 244 619 11 Fragments arranged by size: 619 244 44 11 BtgI C'CryG_G Cuts at: 0 776 918 Size: 776 142 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 90 918 Size: 90 828 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 372 407 918 Size: 372 35 511 Fragments arranged by size: 511 372 35 EaeI y'GGCC_r Cuts at: 0 380 535 683 918 Size: 380 155 148 235 Fragments arranged by size: 380 235 155 148 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 145 304 721 892 918 Size: 145 159 417 171 26 Fragments arranged by size: 417 171 159 145 26 HaeII r_GCGC'y Cuts at: 0 548 708 800 918 Size: 548 160 92 118 Fragments arranged by size: 548 160 118 92 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 247 279 420 452 918 Size: 247 32 141 32 466 Fragments arranged by size: 466 247 141 32 32 HindIII A'AGCT_T Cuts at: 0 98 874 918 Size: 98 776 44 Fragments arranged by size: 776 98 44 MscI TGG'CCA Cuts at: 0 382 685 918 Size: 382 303 233 Fragments arranged by size: 382 303 233 MslI CAynn'nnrTG Cuts at: 0 778 918 Size: 778 140 NcoI C'CATG_G Cuts at: 0 776 918 Size: 776 142 NdeI CA'TA_TG Cuts at: 0 480 918 Size: 480 438 NspI r_CATG'y Cuts at: 0 235 918 Size: 235 683 ScaI AGT'ACT Cuts at: 0 317 918 Size: 317 601 SfcI C'TryA_G Cuts at: 0 712 903 918 Size: 712 191 15 Fragments arranged by size: 712 191 15 SmlI C'TyrA_G Cuts at: 0 292 918 Size: 292 626 StyI C'CwwG_G Cuts at: 0 360 680 776 918 Size: 360 320 96 142 Fragments arranged by size: 360 320 142 96 TatI w'GTAC_w Cuts at: 0 315 450 918 Size: 315 135 468 Fragments arranged by size: 468 315 135 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 588 620 918 Size: 588 32 298 Fragments arranged by size: 588 298 32 ZraI GAC'GTC Cuts at: 0 504 918 Size: 504 414 Enzymes that do cut: AatII AcuI AfeI AleI AlfI ApoI AvaI BamHI BanI BcgI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaAI BsaHI BsaWI BsaXI BsiHKAI BsmBI Bsp1286I BspEI BsrBI BsrGI BssSI BstYI BtgI BtgZI CspCI EaeI Eco57MI HaeII Hin4I HindIII MscI MslI NcoI NdeI NspI ScaI SfcI SmlI StyI TatI TstI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AflII AflIII AgeI AhdI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BmtI Bpu10I BsaI BsaBI BseRI BseYI BsgI BsiEI BsiWI BsmI BspHI BspMI BsrDI BsrFI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI