Restriction map for pF1KB6407
 (Linear) (Six-base) MAPSORT of: KIBB6407.seq  Check: 7415  from: 1  to: 918



KIBB6407.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AatII G_ACGT'C

Cuts at:      0     506     918 
   Size:        506     412
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     892     918 
   Size:        892      26
 
AfeI AGC'GCT

Cuts at:      0     706     918 
   Size:        706     212
 
AleI CACnn'nnGTG

Cuts at:      0     778     918 
   Size:        778     140
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     796     918 
   Size:        796     122
 
ApoI r'AATT_y

Cuts at:      0     130     918 
   Size:        130     788
 
AvaI C'yCGr_G

Cuts at:      0      61     638     918 
   Size:         61     577     280

  Fragments arranged by size: 

                 577     280      61
 
BamHI G'GATC_C

Cuts at:      0      44     918 
   Size:         44     874
 
BanI G'GyrC_C

Cuts at:      0     780     918 
   Size:        780     138
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      42      76     918 
   Size:         42      34     842

  Fragments arranged by size: 

                 842      42      34
 
BglII A'GATC_T

Cuts at:      0     288     907     918 
   Size:        288     619      11

  Fragments arranged by size: 

                 619     288      11
 
BlpI GC'TnA_GC

Cuts at:      0     541     769     918 
   Size:        541     228     149

  Fragments arranged by size: 

                 541     228     149
 
Bme1580I G_kGCm'C

Cuts at:      0     785     918 
   Size:        785     133
 
BmgBI CAC'GTC

Cuts at:      0     565     918 
   Size:        565     353
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     395     918 
   Size:        395     523
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     541     918 
   Size:        541     377
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     304     721     918 
   Size:        145     159     417     197

  Fragments arranged by size: 

                 417     197     159     145
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     918 
   Size:        313     605
 
BsaAI yAC'GTr

Cuts at:      0      39     575     918 
   Size:         39     536     343

  Fragments arranged by size: 

                 536     343      39
 
BsaHI Gr'CG_yC

Cuts at:      0      57     503     918 
   Size:         57     446     415

  Fragments arranged by size: 

                 446     415      57
 
BsaWI w'CCGG_w

Cuts at:      0      47     918 
   Size:         47     871
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     247     277     588     618     918 
   Size:        247      30     311      30     300

  Fragments arranged by size: 

                 311     300     247      30      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     775     917     918 
   Size:        775     142       1

  Fragments arranged by size: 

                 775     142       1
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     635     918 
   Size:        635     283
 
Bsp1286I G_dGCh'C

Cuts at:      0     775     785     917     918 
   Size:        775      10     132       1

  Fragments arranged by size: 

                 775     132      10       1
 
BspEI T'CCGG_A

Cuts at:      0      47     918 
   Size:         47     871
 
BsrBI CCG'CTC

Cuts at:      0     756     918 
   Size:        756     162
 
BsrGI T'GTAC_A

Cuts at:      0     450     918 
   Size:        450     468
 
BssSI C'ACGA_G

Cuts at:      0     700     918 
   Size:        700     218
 
BstYI r'GATC_y

Cuts at:      0      44     288     907     918 
   Size:         44     244     619      11

  Fragments arranged by size: 

                 619     244      44      11
 
BtgI C'CryG_G

Cuts at:      0     776     918 
   Size:        776     142
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      90     918 
   Size:         90     828
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     372     407     918 
   Size:        372      35     511

  Fragments arranged by size: 

                 511     372      35
 
EaeI y'GGCC_r

Cuts at:      0     380     535     683     918 
   Size:        380     155     148     235

  Fragments arranged by size: 

                 380     235     155     148
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     304     721     892     918 
   Size:        145     159     417     171      26

  Fragments arranged by size: 

                 417     171     159     145      26
 
HaeII r_GCGC'y

Cuts at:      0     548     708     800     918 
   Size:        548     160      92     118

  Fragments arranged by size: 

                 548     160     118      92
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     247     279     420     452     918 
   Size:        247      32     141      32     466

  Fragments arranged by size: 

                 466     247     141      32      32
 
HindIII A'AGCT_T

Cuts at:      0      98     874     918 
   Size:         98     776      44

  Fragments arranged by size: 

                 776      98      44
 
MscI TGG'CCA

Cuts at:      0     382     685     918 
   Size:        382     303     233

  Fragments arranged by size: 

                 382     303     233
 
MslI CAynn'nnrTG

Cuts at:      0     778     918 
   Size:        778     140
 
NcoI C'CATG_G

Cuts at:      0     776     918 
   Size:        776     142
 
NdeI CA'TA_TG

Cuts at:      0     480     918 
   Size:        480     438
 
NspI r_CATG'y

Cuts at:      0     235     918 
   Size:        235     683
 
ScaI AGT'ACT

Cuts at:      0     317     918 
   Size:        317     601
 
SfcI C'TryA_G

Cuts at:      0     712     903     918 
   Size:        712     191      15

  Fragments arranged by size: 

                 712     191      15
 
SmlI C'TyrA_G

Cuts at:      0     292     918 
   Size:        292     626
 
StyI C'CwwG_G

Cuts at:      0     360     680     776     918 
   Size:        360     320      96     142

  Fragments arranged by size: 

                 360     320     142      96
 
TatI w'GTAC_w

Cuts at:      0     315     450     918 
   Size:        315     135     468

  Fragments arranged by size: 

                 468     315     135
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     588     620     918 
   Size:        588      32     298

  Fragments arranged by size: 

                 588     298      32
 
ZraI GAC'GTC

Cuts at:      0     504     918 
   Size:        504     414
 

 Enzymes that do cut:

    AatII     AcuI     AfeI     AleI     AlfI     ApoI     AvaI    BamHI
     BanI     BcgI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BsrBI    BsrGI    BssSI    BstYI     BtgI    BtgZI
    CspCI     EaeI  Eco57MI    HaeII    Hin4I  HindIII     MscI     MslI
     NcoI     NdeI     NspI     ScaI     SfcI     SmlI     StyI     TatI
     TstI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI
     BglI     BmtI   Bpu10I     BsaI    BsaBI    BseRI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BspHI    BspMI    BsrDI    BsrFI   BssHII
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtsI     ClaI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI
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