(Linear) (Six-base) MAPSORT of: KIBB6409.seq  Check: 9804  from: 1  to: 921



KIBB6409.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     862     921 
   Size:        862      59
 
AlwNI CAG_nnn'CTG

Cuts at:      0     496     921 
   Size:        496     425
 
ApaLI G'TGCA_C

Cuts at:      0     650     921 
   Size:        650     271
 
ApoI r'AATT_y

Cuts at:      0     172     921 
   Size:        172     749
 
BanII G_rGCy'C

Cuts at:      0     816     921 
   Size:        816     105
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     806     921 
   Size:        806     115
 
BclI T'GATC_A

Cuts at:      0     341     921 
   Size:        341     580
 
Bme1580I G_kGCm'C

Cuts at:      0     264     654     921 
   Size:        264     390     267

  Fragments arranged by size: 

                 390     267     264
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      86     921 
   Size:         86     835
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     563     644     921 
   Size:        563      81     277

  Fragments arranged by size: 

                 563     277      81
 
Bpu10I CC'TnA_GC

Cuts at:      0      71     921 
   Size:         71     850
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     470     880     921 
   Size:        227     243     410      41

  Fragments arranged by size: 

                 410     243     227      41
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      83     921 
   Size:         83     838
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     473     921 
   Size:        473     448
 
BsiHKAI G_wGCw'C

Cuts at:      0     654     816     921 
   Size:        654     162     105

  Fragments arranged by size: 

                 654     162     105
 
Bsp1286I G_dGCh'C

Cuts at:      0     264     654     816     921 
   Size:        264     390     162     105

  Fragments arranged by size: 

                 390     264     162     105
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     747     921 
   Size:        747     174
 
BsrBI CCG'CTC

Cuts at:      0      27     921 
   Size:         27     894
 
BsrDI GCAATG_nn'

Cuts at:      0     131     921 
   Size:        131     790
 
BtsI GCAGTG_nn'

Cuts at:      0     846     866     921 
   Size:        846      20      55

  Fragments arranged by size: 

                 846      55      20
 
ClaI AT'CG_AT

Cuts at:      0     146     921 
   Size:        146     775
 
DraI TTT'AAA

Cuts at:      0     467     921 
   Size:        467     454
 
EaeI y'GGCC_r

Cuts at:      0       2     921 
   Size:          2     919
 
EcoICRI GAG'CTC

Cuts at:      0     814     921 
   Size:        814     107
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     563     644     921 
   Size:        563      81     277

  Fragments arranged by size: 

                 563     277      81
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     488     921 
   Size:        488     433
 
EcoO109I rG'GnC_Cy

Cuts at:      0     493     921 
   Size:        493     428
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     480     741     921 
   Size:        480     261     180

  Fragments arranged by size: 

                 480     261     180
 
MspA1I CmG'CkG

Cuts at:      0      18     921 
   Size:         18     903
 
NspI r_CATG'y

Cuts at:      0     612     921 
   Size:        612     309
 
PasI CC'CwG_GG

Cuts at:      0     774     921 
   Size:        774     147
 
SacI G_AGCT'C

Cuts at:      0     816     921 
   Size:        816     105
 
ScaI AGT'ACT

Cuts at:      0     823     921 
   Size:        823      98
 
SmlI C'TyrA_G

Cuts at:      0     242     485     895     921 
   Size:        242     243     410      26

  Fragments arranged by size: 

                 410     243     242      26
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     211     921 
   Size:        211     710
 
TatI w'GTAC_w

Cuts at:      0     821     921 
   Size:        821     100
 

 Enzymes that do cut:

     AlfI    AlwNI    ApaLI     ApoI    BanII     BbsI     BclI Bme1580I
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BseRI  BsiHKAI Bsp1286I
    BspMI    BsrBI    BsrDI     BtsI     ClaI     DraI     EaeI  EcoICRI
  Eco57MI    EcoNI EcoO109I     MmeI   MspA1I     NspI     PasI     SacI
     ScaI     SmlI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI     ApaI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI    CspCI   DraIII     DrdI     EagI     EarI
     EciI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI