Restriction map for
pF1KB6411
(Linear) (Six-base) MAPSORT of: KIBB6411.seq Check: 4319 from: 1 to: 921
KIBB6411.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AatII G_ACGT'C
Cuts at: 0 215 921
Size: 215 706
AclI AA'CG_TT
Cuts at: 0 277 921
Size: 277 644
AflII C'TTAA_G
Cuts at: 0 298 921
Size: 298 623
AlwNI CAG_nnn'CTG
Cuts at: 0 519 921
Size: 519 402
ApaI G_GGCC'C
Cuts at: 0 572 921
Size: 572 349
ApaLI G'TGCA_C
Cuts at: 0 487 921
Size: 487 434
AvaI C'yCGr_G
Cuts at: 0 629 921
Size: 629 292
BanI G'GyrC_C
Cuts at: 0 555 774 921
Size: 555 219 147
Fragments arranged by size:
555 219 147
BanII G_rGCy'C
Cuts at: 0 56 572 628 921
Size: 56 516 56 293
Fragments arranged by size:
516 293 56 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 608 921
Size: 608 313
BbvCI CC'TCA_GC
Cuts at: 0 448 921
Size: 448 473
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 25 59 921
Size: 25 34 862
Fragments arranged by size:
862 34 25
BglII A'GATC_T
Cuts at: 0 101 434 476 921
Size: 101 333 42 445
Fragments arranged by size:
445 333 101 42
Bme1580I G_kGCm'C
Cuts at: 0 491 572 711 921
Size: 491 81 139 210
Fragments arranged by size:
491 210 139 81
BmgBI CAC'GTC
Cuts at: 0 660 921
Size: 660 261
BmrI ACTGGGnnnn_n'
Cuts at: 0 663 921
Size: 663 258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 921
Size: 820 101
Bpu10I CC'TnA_GC
Cuts at: 0 89 448 921
Size: 89 359 473
Fragments arranged by size:
473 359 89
BsaI GGTCTCn'nnnn_
Cuts at: 0 214 921
Size: 214 707
BsaHI Gr'CG_yC
Cuts at: 0 212 921
Size: 212 709
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 574 647 921
Size: 127 447 73 274
Fragments arranged by size:
447 274 127 73
BseYI C'CCAG_C
Cuts at: 0 614 921
Size: 614 307
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 411 921
Size: 145 266 510
Fragments arranged by size:
510 266 145
BsiEI CG_ry'CG
Cuts at: 0 543 921
Size: 543 378
BsiHKAI G_wGCw'C
Cuts at: 0 56 491 761 921
Size: 56 435 270 160
Fragments arranged by size:
435 270 160 56
BsmBI CGTCTCn'nnnn_
Cuts at: 0 745 921
Size: 745 176
Bsp1286I G_dGCh'C
Cuts at: 0 56 491 572 628 711 761 921
Size: 56 435 81 56 83 50 160
Fragments arranged by size:
435 160 83 81 56 56 50
BspHI T'CATG_A
Cuts at: 0 561 921
Size: 561 360
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 354 921
Size: 354 567
BsrFI r'CCGG_y
Cuts at: 0 23 637 921
Size: 23 614 284
Fragments arranged by size:
614 284 23
BstAPI GCAn_nnn'nTGC
Cuts at: 0 555 921
Size: 555 366
BstYI r'GATC_y
Cuts at: 0 101 434 476 921
Size: 101 333 42 445
Fragments arranged by size:
445 333 101 42
BtgI C'CryG_G
Cuts at: 0 165 255 902 921
Size: 165 90 647 19
Fragments arranged by size:
647 165 90 19
BtsI GCAGTG_nn'
Cuts at: 0 660 921
Size: 660 261
ClaI AT'CG_AT
Cuts at: 0 467 921
Size: 467 454
DraIII CAC_nnn'GTG
Cuts at: 0 152 767 921
Size: 152 615 154
Fragments arranged by size:
615 154 152
DrdI GACnn_nn'nnGTC
Cuts at: 0 142 921
Size: 142 779
EaeI y'GGCC_r
Cuts at: 0 905 921
Size: 905 16
EarI CTCTTCn'nnn_
Cuts at: 0 238 921
Size: 238 683
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 921
Size: 19 902
EcoICRI GAG'CTC
Cuts at: 0 54 921
Size: 54 867
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 921
Size: 820 101
EcoO109I rG'GnC_Cy
Cuts at: 0 78 495 921
Size: 78 417 426
Fragments arranged by size:
426 417 78
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 759 921
Size: 759 162
FspI TGC'GCA
Cuts at: 0 394 921
Size: 394 527
HaeII r_GCGC'y
Cuts at: 0 423 921
Size: 423 498
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 507 539 921
Size: 507 32 382
Fragments arranged by size:
507 382 32
MslI CAynn'nnrTG
Cuts at: 0 694 746 921
Size: 694 52 175
Fragments arranged by size:
694 175 52
MspA1I CmG'CkG
Cuts at: 0 39 794 921
Size: 39 755 127
Fragments arranged by size:
755 127 39
NaeI GCC'GGC
Cuts at: 0 639 921
Size: 639 282
NcoI C'CATG_G
Cuts at: 0 165 921
Size: 165 756
NgoMIV G'CCGG_C
Cuts at: 0 637 921
Size: 637 284
PflMI CCAn_nnn'nTGG
Cuts at: 0 519 614 717 921
Size: 519 95 103 204
Fragments arranged by size:
519 204 103 95
PspOMI G'GGCC_C
Cuts at: 0 568 921
Size: 568 353
PvuII CAG'CTG
Cuts at: 0 39 921
Size: 39 882
SacI G_AGCT'C
Cuts at: 0 56 921
Size: 56 865
SfcI C'TryA_G
Cuts at: 0 264 921
Size: 264 657
SmlI C'TyrA_G
Cuts at: 0 298 921
Size: 298 623
StyI C'CwwG_G
Cuts at: 0 165 921
Size: 165 756
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 557 792 921
Size: 557 235 129
Fragments arranged by size:
557 235 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 869 901 921
Size: 869 32 20
Fragments arranged by size:
869 32 20
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 102 516 819 900 921
Size: 102 414 303 81 21
Fragments arranged by size:
414 303 102 81 21
XmnI GAAnn'nnTTC
Cuts at: 0 844 921
Size: 844 77
ZraI GAC'GTC
Cuts at: 0 213 921
Size: 213 708
Enzymes that do cut:
AatII AclI AflII AlwNI ApaI ApaLI AvaI BanI
BanII BbsI BbvCI BcgI BglII Bme1580I BmgBI BmrI
BpmI Bpu10I BsaI BsaHI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrFI BstAPI BstYI
BtgI BtsI ClaI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I FalI FspI HaeII Hin4I MslI
MspA1I NaeI NcoI NgoMIV PflMI PspOMI PvuII SacI
SfcI SmlI StyI TaqII TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BciVI BclI BfrBI BglI BlpI
BmtI BplI BpuEI BsaAI BsaBI BsaWI BsaXI BsiWI
BsmI BspEI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI CspCI DraI EagI
EcoNI EcoRI EcoRV FseI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NarI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XbaI XhoI XmaI