Restriction map for
pF1KB6412
(Linear) (Six-base) MAPSORT of: KIBB6412.seq Check: 469 from: 1 to: 1005
KIBB6412.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 52 1005
Size: 52 953
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 707 816 1005
Size: 440 267 109 189
Fragments arranged by size:
440 267 189 109
AleI CACnn'nnGTG
Cuts at: 0 148 497 821 1005
Size: 148 349 324 184
Fragments arranged by size:
349 324 184 148
AlwNI CAG_nnn'CTG
Cuts at: 0 402 432 1005
Size: 402 30 573
Fragments arranged by size:
573 402 30
ApaI G_GGCC'C
Cuts at: 0 604 1005
Size: 604 401
AvaI C'yCGr_G
Cuts at: 0 525 1005
Size: 525 480
BamHI G'GATC_C
Cuts at: 0 452 1005
Size: 452 553
BanI G'GyrC_C
Cuts at: 0 392 1005
Size: 392 613
BanII G_rGCy'C
Cuts at: 0 532 604 1005
Size: 532 72 401
Fragments arranged by size:
532 401 72
BbsI GAAGACnn'nnnn_
Cuts at: 0 220 1005
Size: 220 785
BbvCI CC'TCA_GC
Cuts at: 0 923 1005
Size: 923 82
BfrBI ATG'CAT
Cuts at: 0 350 1005
Size: 350 655
Bme1580I G_kGCm'C
Cuts at: 0 604 1005
Size: 604 401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 631 1005
Size: 199 432 374
Fragments arranged by size:
432 374 199
Bpu10I CC'TnA_GC
Cuts at: 0 229 923 1005
Size: 229 694 82
Fragments arranged by size:
694 229 82
BsaHI Gr'CG_yC
Cuts at: 0 474 827 1005
Size: 474 353 178
Fragments arranged by size:
474 353 178
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 672 911 985 1005
Size: 672 239 74 20
Fragments arranged by size:
672 239 74 20
BseYI C'CCAG_C
Cuts at: 0 51 1005
Size: 51 954
BsiEI CG_ry'CG
Cuts at: 0 149 1005
Size: 149 856
BsmI GAATG_Cn'
Cuts at: 0 352 1005
Size: 352 653
Bsp1286I G_dGCh'C
Cuts at: 0 532 604 1005
Size: 532 72 401
Fragments arranged by size:
532 401 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 52 1005
Size: 52 953
BsrFI r'CCGG_y
Cuts at: 0 389 742 1005
Size: 389 353 263
Fragments arranged by size:
389 353 263
BstEII G'GTnAC_C
Cuts at: 0 4 441 1005
Size: 4 437 564
Fragments arranged by size:
564 437 4
BstXI CCAn_nnnn'nTGG
Cuts at: 0 59 499 1005
Size: 59 440 506
Fragments arranged by size:
506 440 59
BstYI r'GATC_y
Cuts at: 0 452 1005
Size: 452 553
BtgI C'CryG_G
Cuts at: 0 498 672 1005
Size: 498 174 333
Fragments arranged by size:
498 333 174
BtsI GCAGTG_nn'
Cuts at: 0 85 412 915 1005
Size: 85 327 503 90
Fragments arranged by size:
503 327 90 85
DrdI GACnn_nn'nnGTC
Cuts at: 0 895 1005
Size: 895 110
EaeI y'GGCC_r
Cuts at: 0 309 636 1005
Size: 309 327 369
Fragments arranged by size:
369 327 309
EarI CTCTTCn'nnn_
Cuts at: 0 104 167 683 1005
Size: 104 63 516 322
Fragments arranged by size:
516 322 104 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 440 631 707 816 1005
Size: 199 241 191 76 109 189
Fragments arranged by size:
241 199 191 189 109 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 124 265 1005
Size: 124 141 740
Fragments arranged by size:
740 141 124
EcoO109I rG'GnC_Cy
Cuts at: 0 63 129 224 600 715 1005
Size: 63 66 95 376 115 290
Fragments arranged by size:
376 290 115 95 66 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 116 148 1005
Size: 116 32 857
Fragments arranged by size:
857 116 32
MslI CAynn'nnrTG
Cuts at: 0 148 497 821 848 1005
Size: 148 349 324 27 157
Fragments arranged by size:
349 324 157 148 27
MspA1I CmG'CkG
Cuts at: 0 15 24 653 1005
Size: 15 9 629 352
Fragments arranged by size:
629 352 15 9
NaeI GCC'GGC
Cuts at: 0 391 1005
Size: 391 614
NcoI C'CATG_G
Cuts at: 0 672 1005
Size: 672 333
NgoMIV G'CCGG_C
Cuts at: 0 389 1005
Size: 389 616
NsiI A_TGCA'T
Cuts at: 0 352 1005
Size: 352 653
NspI r_CATG'y
Cuts at: 0 78 814 1005
Size: 78 736 191
Fragments arranged by size:
736 191 78
PasI CC'CwG_GG
Cuts at: 0 120 1005
Size: 120 885
PflMI CCAn_nnn'nTGG
Cuts at: 0 980 1005
Size: 980 25
PfoI T'CCnGG_A
Cuts at: 0 455 608 1005
Size: 455 153 397
Fragments arranged by size:
455 397 153
PpuMI rG'GwC_Cy
Cuts at: 0 129 224 1005
Size: 129 95 781
Fragments arranged by size:
781 129 95
PspOMI G'GGCC_C
Cuts at: 0 600 1005
Size: 600 405
PstI C_TGCA'G
Cuts at: 0 139 407 1005
Size: 139 268 598
Fragments arranged by size:
598 268 139
PvuI CG_AT'CG
Cuts at: 0 149 1005
Size: 149 856
PvuII CAG'CTG
Cuts at: 0 653 1005
Size: 653 352
SapI GCTCTTCn'nnn_
Cuts at: 0 167 1005
Size: 167 838
SfcI C'TryA_G
Cuts at: 0 135 403 1005
Size: 135 268 602
Fragments arranged by size:
602 268 135
SmaI CCC'GGG
Cuts at: 0 527 1005
Size: 527 478
SphI G_CATG'C
Cuts at: 0 78 1005
Size: 78 927
SrfI GCCC'GGGC
Cuts at: 0 527 1005
Size: 527 478
StyI C'CwwG_G
Cuts at: 0 672 1005
Size: 672 333
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 685 811 899 1005
Size: 685 126 88 106
Fragments arranged by size:
685 126 106 88
TatI w'GTAC_w
Cuts at: 0 862 1005
Size: 862 143
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 304 907 1005
Size: 304 603 98
Fragments arranged by size:
603 304 98
XmaI C'CCGG_G
Cuts at: 0 525 1005
Size: 525 480
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaI AvaI BamHI BanI
BanII BbsI BbvCI BfrBI Bme1580I BpmI Bpu10I BsaHI
BseRI BseYI BsiEI BsmI Bsp1286I BspMI BsrFI BstEII
BstXI BstYI BtgI BtsI DrdI EaeI EarI Eco57MI
EcoNI EcoO109I Hin4I MslI MspA1I NaeI NcoI NgoMIV
NsiI NspI PasI PflMI PfoI PpuMI PspOMI PstI
PvuI PvuII SapI SfcI SmaI SphI SrfI StyI
TaqII TatI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BcgI BciVI BclI BglI BglII
BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BsgI BsiHKAI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NarI NdeI NheI NotI NruI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SspI
StuI SwaI TstI Tth111I XbaI XhoI XmnI ZraI