Restriction map for
pF1KB6413
(Linear) (Six-base) MAPSORT of: KIBB6413.seq Check: 3200 from: 1 to: 924
KIBB6413.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 507 924
Size: 507 417
AatII G_ACGT'C
Cuts at: 0 575 924
Size: 575 349
AccI GT'mk_AC
Cuts at: 0 869 924
Size: 869 55
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 900 924
Size: 289 611 24
Fragments arranged by size:
611 289 24
AflIII A'CryG_T
Cuts at: 0 329 924
Size: 329 595
AlwNI CAG_nnn'CTG
Cuts at: 0 813 924
Size: 813 111
ApaI G_GGCC'C
Cuts at: 0 585 924
Size: 585 339
AvaI C'yCGr_G
Cuts at: 0 421 924
Size: 421 503
BanI G'GyrC_C
Cuts at: 0 80 705 924
Size: 80 625 219
Fragments arranged by size:
625 219 80
BanII G_rGCy'C
Cuts at: 0 43 585 924
Size: 43 542 339
Fragments arranged by size:
542 339 43
BfrBI ATG'CAT
Cuts at: 0 843 924
Size: 843 81
Bme1580I G_kGCm'C
Cuts at: 0 585 924
Size: 585 339
BmrI ACTGGGnnnn_n'
Cuts at: 0 635 783 846 882 924
Size: 635 148 63 36 42
Fragments arranged by size:
635 148 63 42 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 924
Size: 148 776
Bpu10I CC'TnA_GC
Cuts at: 0 233 656 924
Size: 233 423 268
Fragments arranged by size:
423 268 233
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 744 924
Size: 133 611 180
Fragments arranged by size:
611 180 133
BsaHI Gr'CG_yC
Cuts at: 0 572 924
Size: 572 352
BsaWI w'CCGG_w
Cuts at: 0 35 924
Size: 35 889
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 331 361 557 587 924
Size: 331 30 196 30 337
Fragments arranged by size:
337 331 196 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 661 924
Size: 205 456 263
Fragments arranged by size:
456 263 205
BseYI C'CCAG_C
Cuts at: 0 43 62 924
Size: 43 19 862
Fragments arranged by size:
862 43 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 426 579 924
Size: 426 153 345
Fragments arranged by size:
426 345 153
BsiEI CG_ry'CG
Cuts at: 0 203 924
Size: 203 721
BsmBI CGTCTCn'nnnn_
Cuts at: 0 112 924
Size: 112 812
Bsp1286I G_dGCh'C
Cuts at: 0 43 585 924
Size: 43 542 339
Fragments arranged by size:
542 339 43
BspEI T'CCGG_A
Cuts at: 0 35 924
Size: 35 889
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 507 641 924
Size: 507 134 283
Fragments arranged by size:
507 283 134
BsrFI r'CCGG_y
Cuts at: 0 77 924
Size: 77 847
BstXI CCAn_nnnn'nTGG
Cuts at: 0 696 924
Size: 696 228
BstYI r'GATC_y
Cuts at: 0 344 924
Size: 344 580
Bsu36I CC'TnA_GG
Cuts at: 0 806 924
Size: 806 118
BtgI C'CryG_G
Cuts at: 0 280 671 709 924
Size: 280 391 38 215
Fragments arranged by size:
391 280 215 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 378 924
Size: 378 546
BtsI GCAGTG_nn'
Cuts at: 0 741 924
Size: 741 183
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 704 739 924
Size: 704 35 185
Fragments arranged by size:
704 185 35
EaeI y'GGCC_r
Cuts at: 0 50 75 200 712 924
Size: 50 25 125 512 212
Fragments arranged by size:
512 212 125 50 25
EagI C'GGCC_G
Cuts at: 0 200 924
Size: 200 724
EarI CTCTTCn'nnn_
Cuts at: 0 524 535 604 919 924
Size: 524 11 69 315 5
Fragments arranged by size:
524 315 69 11 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 289 900 924
Size: 148 141 611 24
Fragments arranged by size:
611 148 141 24
EcoO109I rG'GnC_Cy
Cuts at: 0 581 660 810 924
Size: 581 79 150 114
Fragments arranged by size:
581 150 114 79
HaeII r_GCGC'y
Cuts at: 0 31 683 924
Size: 31 652 241
Fragments arranged by size:
652 241 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 316 348 557 589 789 821
Size: 181 32 103 32 209 32 200 32
Cuts at: 821 924
Size: 103
Fragments arranged by size:
209 200 181 103 103 32 32 32
32
HincII GTy'rAC
Cuts at: 0 870 924
Size: 870 54
MscI TGG'CCA
Cuts at: 0 52 924
Size: 52 872
MslI CAynn'nnrTG
Cuts at: 0 694 924
Size: 694 230
MspA1I CmG'CkG
Cuts at: 0 666 711 893 924
Size: 666 45 182 31
Fragments arranged by size:
666 182 45 31
NaeI GCC'GGC
Cuts at: 0 79 924
Size: 79 845
NgoMIV G'CCGG_C
Cuts at: 0 77 924
Size: 77 847
NsiI A_TGCA'T
Cuts at: 0 845 924
Size: 845 79
NspI r_CATG'y
Cuts at: 0 843 924
Size: 843 81
PfoI T'CCnGG_A
Cuts at: 0 812 924
Size: 812 112
PpuMI rG'GwC_Cy
Cuts at: 0 810 924
Size: 810 114
PspOMI G'GGCC_C
Cuts at: 0 581 924
Size: 581 343
PspXI vC'TCGA_Gb
Cuts at: 0 421 924
Size: 421 503
PstI C_TGCA'G
Cuts at: 0 257 924
Size: 257 667
SacII CC_GC'GG
Cuts at: 0 712 924
Size: 712 212
SalI G'TCGA_C
Cuts at: 0 868 924
Size: 868 56
SapI GCTCTTCn'nnn_
Cuts at: 0 604 924
Size: 604 320
SfcI C'TryA_G
Cuts at: 0 227 253 924
Size: 227 26 671
Fragments arranged by size:
671 227 26
SmlI C'TyrA_G
Cuts at: 0 10 148 421 723 924
Size: 10 138 273 302 201
Fragments arranged by size:
302 273 201 138 10
StuI AGG'CCT
Cuts at: 0 490 924
Size: 490 434
StyI C'CwwG_G
Cuts at: 0 182 524 924
Size: 182 342 400
Fragments arranged by size:
400 342 182
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 453 924
Size: 453 471
XhoI C'TCGA_G
Cuts at: 0 421 924
Size: 421 503
ZraI GAC'GTC
Cuts at: 0 573 924
Size: 573 351
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AlwNI ApaI AvaI
BanI BanII BfrBI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmBI
Bsp1286I BspEI BspMI BsrFI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI EaeI EagI EarI Eco57MI EcoO109I
HaeII Hin4I HincII MscI MslI MspA1I NaeI NgoMIV
NsiI NspI PfoI PpuMI PspOMI PspXI PstI SacII
SalI SapI SfcI SmlI StuI StyI XcmI XhoI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI
BsiHKAI BsiWI BsmI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I ClaI DraI DraIII
DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
MmeI NarI NcoI NdeI NheI NotI NruI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PsrI PvuI PvuII RsrII SacI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI TstI Tth111I
XbaI XmaI XmnI